LOCUS BBa_K1426000 3348 bp DNA linear 22-Dec-2024 DEFINITION pLac-RBS-RFP-term-XylR-pXyl-RBS-GFP-2term ACCESSION BBa_K1426000 VERSION BBa_K1426000.1 FEATURES Location/Qualifiers CDS 7..1173 /label=xylR promoter 1300..1305 /label=Sigma A, -10 promoter 1277..1282 /label=Sigma A, -35 promoter 1..1387 /label=BBa_K733002 RBS 1396..1408 /label=RBS-3\Weak RBS 1402..1405 /label=RBS misc_feature 1396..1408 /label=BBa_B0032 RBS 1396..1408 /label=BBa_B0032 CDS 1415..2134 /label=GFP protein CDS 1415..2134 /label=BBa_E0040 stem_loop 2154..2197 /label=stem_loop misc_feature 2143..2222 /label=BBa_B0010 terminator 2143..2222 /label=BBa_B0010 stem_loop 2238..2257 /label=T7 TE misc_feature 2264..2264 /label=stop polyA_site 2258..2271 /label=polya misc_feature 2231..2271 /label=BBa_B0012 terminator 2231..2271 /label=BBa_B0012 misc_feature 1396..2271 /label=BBa_E0240 misc_feature 1..2271 /label=BBa_K733018 CDS 2280..2367 /label=end of LacI coding region (inactive) misc_feature 2280..2479 /label=BBa_R0010 misc_feature 2368..2405 /label=CAP binding site promoter 2416..2421 /label=-35 promoter 2440..2445 /label=-10 misc_feature 2445..2479 /label=LacI binding site misc_feature 2452..2452 /label=start promoter 2280..2479 /label=BBa_R0010 misc_feature 2492..2495 /label=conserved RBS 2488..2499 /label=BBa_B0034 CDS 2506..3180 /label=mrfp1 misc_feature 3187..3211 /label=Help:Barcodes CDS 2506..3186 /label=BBa_E1010 stem_loop 3231..3274 /label=stem_loop misc_feature 3220..3299 /label=BBa_B0010 terminator 3220..3299 /label=BBa_B0010 stem_loop 3315..3334 /label=T7 TE misc_feature 3341..3341 /label=stop polyA_site 3335..3348 /label=polya misc_feature 3308..3348 /label=BBa_B0012 terminator 3308..3348 /label=BBa_B0012 misc_feature 2280..3348 /label=BBa_J04450 ORIGIN 1 gagctcctaa cttatagggg taacacttaa aaaagaatca ataacgatag aaaccgctcc 61 taaagcaggt gcattttttc ctaacgaaga aggcaatagt tcacatttat tgtctaaatg 121 agaatggact cttgaagaaa cttcgttttt aatcgtattt aaaacaatgg gatgagattc 181 aattatatga tttctcaaga taacagcttc tatatcaaat gtattaagga tattggttaa 241 tccaattccg atataaaagc caaagttttg aagtgcattt aacatttcta catcattttt 301 atttgcgcgt tccacaatct cttttcgaga aatattcttt tcttctttag agagcgaagc 361 cagtaacgct ttttcagaag catataattc ccaacagcct cgatttccac agctgcattt 421 gggtccatta aaatctatcg tcatatgacc catttcccca gaaaaaccct gaacaccttt 481 atacaattcg ttgttaataa caagtccagt tccaattccg atattaatac tgatgtaaac 541 gatgttttca tagttttttg tcataccaaa tactttttca ccgtatgctc ctgcattagc 601 ttcattttca acaaaaaccg gaacattaaa ctcactctca attaaaaact gcaaatcttt 661 gatattccaa tttaagttag gcatgaaaat aatttgctga tgacgatcta caaggcctgg 721 aacacaaatt cctattccga ctagaccata aggggactca ggcatatggg ttacaaaacc 781 atgaataagt gcaaataaaa tctcttttac ttcactagcg gaagaactag acaagtcaga 841 agtcttctcg agaataatat ttccttctaa gtcggttaga atgccgttaa gatagtcgac 901 tcctatatca ataccaatcg agtagcctgc attcttatta aaaacaagca ttacaggtct 961 tctgccgcct cttgattgcc ctgccccaat ttcaaaaata aaatcttttt caagcagtgt 1021 atttacttga gaggagacag tagacttgtt taatcctgta atctcagaga gagttgccct 1081 ggagacaggg gagttcttca aaatttcatc taatattaat ttttgattca ttttttttac 1141 taaagcttga tctgcaattt gaataataac cactcctttg tttatccacc gaactaagtt 1201 ggtgtttttt gaagcttgaa ttagatattt aaaagtatca tatctaatat tataactaaa 1261 ttttctaaaa aaaacattga aataaacatt tattttgtat atgatgagat aaagttagtt 1321 tattggataa acaaactaac tcaattaaga tagttgatgg ataaacttgt tcacttaaat 1381 caaagggtac tagagtcaca caggaaagta ctagatgcgt aaaggagaag aacttttcac 1441 tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt 1501 cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac 1561 tactggaaaa ctacctgttc catggccaac acttgtcact actttcggtt atggtgttca 1621 atgctttgcg agatacccag atcatatgaa acagcatgac tttttcaaga gtgccatgcc 1681 cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg 1741 tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga 1801 ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa 1861 tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttaacttca aaattagaca 1921 caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata ctccaattgg 1981 cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa 2041 agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat 2101 tacacatggc atggatgaac tatacaaata ataatactag agccaggcat caaataaaac 2161 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc 2221 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagagc 2281 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag 2341 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca 2401 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag 2461 cggataacaa tttcacacat actagagaaa gaggagaaat actagatggc ttcctccgaa 2521 gacgttatca aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt taacggtcac 2581 gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca gaccgctaaa 2641 ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc cccgcagttc 2701 cagtacggtt ccaaagctta cgttaaacac ccggctgaca tcccggacta cctgaaactg 2761 tccttcccgg aaggtttcaa atgggaacgt gttatgaact tcgaagacgg tggtgttgtt 2821 accgttaccc aggactcctc cctgcaagac ggtgagttca tctacaaagt taaactgcgt 2881 ggtaccaact tcccgtccga cggtccggtt atgcagaaaa aaaccatggg ttgggaagct 2941 tccaccgaac gtatgtaccc ggaagacggt gctctgaaag gtgaaatcaa aatgcgtctg 3001 aaactgaaag acggtggtca ctacgacgct gaagttaaaa ccacctacat ggctaaaaaa 3061 ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac tggacatcac ctcccacaac 3121 gaagactaca ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc caccggtgct 3181 taataacgct gatagtgcta gtgtagatcg ctactagagc caggcatcaa ataaaacgaa 3241 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 3301 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata //