##gff-version 3
##sequence-region BBa_K1426000 1 3348
BBa_K1426000	.	engineered_region	2280	3348	.	+	0	ID=BBa_J04450;Name=BBa_J04450
BBa_K1426000	.	ribosome_entry_site	2488	2499	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04450
BBa_K1426000	.	conserved_region	2492	2495	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1426000	.	CDS	2506	3186	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04450
BBa_K1426000	.	engineered_tag	3187	3211	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1426000	.	CDS	2506	3180	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1426000	.	promoter	2280	2479	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04450
BBa_K1426000	.	engineered_region	2280	2479	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1426000	.	CDS	2280	2367	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1426000	.	promoter	2440	2445	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1426000	.	start_codon	2452	2452	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1426000	.	non_covalent_binding_site	2445	2479	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1426000	.	non_covalent_binding_site	2368	2405	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1426000	.	promoter	2416	2421	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1426000	.	terminator	3220	3299	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04450
BBa_K1426000	.	stem_loop	3231	3274	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1426000	.	engineered_region	3220	3299	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1426000	.	terminator	3308	3348	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04450
BBa_K1426000	.	stem_loop	3315	3334	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1426000	.	stop_codon	3341	3341	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1426000	.	polyA_site	3335	3348	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1426000	.	engineered_region	3308	3348	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1426000	.	engineered_region	1	2271	.	+	0	ID=BBa_K733018;Name=BBa_K733018
BBa_K1426000	.	promoter	1	1387	.	+	0	ID=BBa_K733002;Name=BBa_K733002;Parent=BBa_K733018
BBa_K1426000	.	promoter	1300	1305	.	+	0	ID=annotation2193603;Name=Sigma A, -10;Parent=BBa_K733002
BBa_K1426000	.	promoter	1277	1282	.	+	0	ID=annotation2193604;Name=Sigma A, -35;Parent=BBa_K733002
BBa_K1426000	.	CDS	7	1173	.	+	0	ID=annotation2193589;Name=xylR;Parent=BBa_K733002
BBa_K1426000	.	engineered_region	1396	2271	.	+	0	ID=BBa_E0240;Name=BBa_E0240;Parent=BBa_K733018
BBa_K1426000	.	terminator	2231	2271	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0240
BBa_K1426000	.	stem_loop	2238	2257	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1426000	.	stop_codon	2264	2264	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1426000	.	polyA_site	2258	2271	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1426000	.	engineered_region	2231	2271	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1426000	.	ribosome_entry_site	1396	1408	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_E0240
BBa_K1426000	.	ribosome_entry_site	1396	1408	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1426000	.	engineered_region	1396	1408	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1426000	.	ribosome_entry_site	1402	1405	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1426000	.	terminator	2143	2222	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0240
BBa_K1426000	.	stem_loop	2154	2197	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1426000	.	engineered_region	2143	2222	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1426000	.	CDS	1415	2134	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0240
BBa_K1426000	.	CDS	1415	2134	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
>BBa_K1426000
gagctcctaacttataggggtaacacttaaaaaagaatcaataacgatagaaaccgctcctaaagcaggtgcatttttt
cctaacgaagaaggcaatagttcacatttattgtctaaatgagaatggactcttgaagaaacttcgtttttaatcgtat
ttaaaacaatgggatgagattcaattatatgatttctcaagataacagcttctatatcaaatgtattaaggatattggt
taatccaattccgatataaaagccaaagttttgaagtgcatttaacatttctacatcatttttatttgcgcgttccaca
atctcttttcgagaaatattcttttcttctttagagagcgaagccagtaacgctttttcagaagcatataattcccaac
agcctcgatttccacagctgcatttgggtccattaaaatctatcgtcatatgacccatttccccagaaaaaccctgaac
acctttatacaattcgttgttaataacaagtccagttccaattccgatattaatactgatgtaaacgatgttttcatag
ttttttgtcataccaaatactttttcaccgtatgctcctgcattagcttcattttcaacaaaaaccggaacattaaact
cactctcaattaaaaactgcaaatctttgatattccaatttaagttaggcatgaaaataatttgctgatgacgatctac
aaggcctggaacacaaattcctattccgactagaccataaggggactcaggcatatgggttacaaaaccatgaataagt
gcaaataaaatctcttttacttcactagcggaagaactagacaagtcagaagtcttctcgagaataatatttccttcta
agtcggttagaatgccgttaagatagtcgactcctatatcaataccaatcgagtagcctgcattcttattaaaaacaag
cattacaggtcttctgccgcctcttgattgccctgccccaatttcaaaaataaaatctttttcaagcagtgtatttact
tgagaggagacagtagacttgtttaatcctgtaatctcagagagagttgccctggagacaggggagttcttcaaaattt
catctaatattaatttttgattcattttttttactaaagcttgatctgcaatttgaataataaccactcctttgtttat
ccaccgaactaagttggtgttttttgaagcttgaattagatatttaaaagtatcatatctaatattataactaaatttt
ctaaaaaaaacattgaaataaacatttattttgtatatgatgagataaagttagtttattggataaacaaactaactca
attaagatagttgatggataaacttgttcacttaaatcaaagggtactagagtcacacaggaaagtactagatgcgtaa
aggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtc
agtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttc
catggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatga
ctttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagaca
cgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaa
acattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaat
caaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactcca
attggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaa
agagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataata
atactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggt
gaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaac
cgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcg
caacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgt
ggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttat
caaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaa
ggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcc
tgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtcctt
cccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctg
caagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaa
ccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaa
actgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgct
tacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaag
gtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacga
aaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggct
caccttcgggtgggcctttctgcgtttata