LOCUS BBa_K1426001 3348 bp DNA linear 25-Nov-2024 DEFINITION pLac-RBS-RFP-term-XylR-pXyl-RBS-GFP-2term ACCESSION BBa_K1426001 VERSION BBa_K1426001.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 CDS 227..901 /label=mrfp1 misc_feature 908..932 /label=Help:Barcodes CDS 227..907 /label=BBa_E1010 stem_loop 952..995 /label=stem_loop misc_feature 941..1020 /label=BBa_B0010 terminator 941..1020 /label=BBa_B0010 stem_loop 1036..1055 /label=T7 TE misc_feature 1062..1062 /label=stop polyA_site 1056..1069 /label=polya misc_feature 1029..1069 /label=BBa_B0012 terminator 1029..1069 /label=BBa_B0012 misc_feature 1..1069 /label=BBa_J04450 CDS 1084..2250 /label=xylR promoter 2377..2382 /label=Sigma A, -10 promoter 2354..2359 /label=Sigma A, -35 promoter 1078..2464 /label=BBa_K733002 RBS 2473..2485 /label=RBS-3\Weak RBS 2479..2482 /label=RBS misc_feature 2473..2485 /label=BBa_B0032 RBS 2473..2485 /label=BBa_B0032 CDS 2492..3211 /label=GFP protein CDS 2492..3211 /label=BBa_E0040 stem_loop 3231..3274 /label=stem_loop misc_feature 3220..3299 /label=BBa_B0010 terminator 3220..3299 /label=BBa_B0010 stem_loop 3315..3334 /label=T7 TE misc_feature 3341..3341 /label=stop polyA_site 3335..3348 /label=polya misc_feature 3308..3348 /label=BBa_B0012 terminator 3308..3348 /label=BBa_B0012 misc_feature 2473..3348 /label=BBa_E0240 misc_feature 1078..3348 /label=BBa_K733018 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg cttcctccga 241 agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca 301 cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa 361 actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt 421 ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact 481 gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt 541 taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg 601 tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc 661 ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct 721 gaaactgaaa gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa 781 accggttcag ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa 841 cgaagactac accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc 901 ttaataacgc tgatagtgct agtgtagatc gctactagag ccaggcatca aataaaacga 961 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 1021 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagaggag 1081 ctcctaactt ataggggtaa cacttaaaaa agaatcaata acgatagaaa ccgctcctaa 1141 agcaggtgca ttttttccta acgaagaagg caatagttca catttattgt ctaaatgaga 1201 atggactctt gaagaaactt cgtttttaat cgtatttaaa acaatgggat gagattcaat 1261 tatatgattt ctcaagataa cagcttctat atcaaatgta ttaaggatat tggttaatcc 1321 aattccgata taaaagccaa agttttgaag tgcatttaac atttctacat catttttatt 1381 tgcgcgttcc acaatctctt ttcgagaaat attcttttct tctttagaga gcgaagccag 1441 taacgctttt tcagaagcat ataattccca acagcctcga tttccacagc tgcatttggg 1501 tccattaaaa tctatcgtca tatgacccat ttccccagaa aaaccctgaa cacctttata 1561 caattcgttg ttaataacaa gtccagttcc aattccgata ttaatactga tgtaaacgat 1621 gttttcatag ttttttgtca taccaaatac tttttcaccg tatgctcctg cattagcttc 1681 attttcaaca aaaaccggaa cattaaactc actctcaatt aaaaactgca aatctttgat 1741 attccaattt aagttaggca tgaaaataat ttgctgatga cgatctacaa ggcctggaac 1801 acaaattcct attccgacta gaccataagg ggactcaggc atatgggtta caaaaccatg 1861 aataagtgca aataaaatct cttttacttc actagcggaa gaactagaca agtcagaagt 1921 cttctcgaga ataatatttc cttctaagtc ggttagaatg ccgttaagat agtcgactcc 1981 tatatcaata ccaatcgagt agcctgcatt cttattaaaa acaagcatta caggtcttct 2041 gccgcctctt gattgccctg ccccaatttc aaaaataaaa tctttttcaa gcagtgtatt 2101 tacttgagag gagacagtag acttgtttaa tcctgtaatc tcagagagag ttgccctgga 2161 gacaggggag ttcttcaaaa tttcatctaa tattaatttt tgattcattt tttttactaa 2221 agcttgatct gcaatttgaa taataaccac tcctttgttt atccaccgaa ctaagttggt 2281 gttttttgaa gcttgaatta gatatttaaa agtatcatat ctaatattat aactaaattt 2341 tctaaaaaaa acattgaaat aaacatttat tttgtatatg atgagataaa gttagtttat 2401 tggataaaca aactaactca attaagatag ttgatggata aacttgttca cttaaatcaa 2461 agggtactag agtcacacag gaaagtacta gatgcgtaaa ggagaagaac ttttcactgg 2521 agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag 2581 tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac 2641 tggaaaacta cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg 2701 ctttgcgaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga 2761 aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc 2821 tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt 2881 taaagaagat ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt 2941 atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa 3001 cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga 3061 tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga 3121 tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac 3181 acatggcatg gatgaactat acaaataata atactagagc caggcatcaa ataaaacgaa 3241 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 3301 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata //