LOCUS       BBa_K1428001            2779 bp    DNA     linear       22-Nov-2024
DEFINITION  Gene cluster for Expressing a purifiable Eut Bacterial 
            Microcompartment
ACCESSION   BBa_K1428001
VERSION     BBa_K1428001.1  
FEATURES             Location/Qualifiers
     promoter        1..19
                     /label=T7 Promoter
     misc_feature    20..44
                     /label=lac operator
     RBS             64..80
                     /label=T7 RBS
     misc_feature    88..90
                     /label=Start codon
     CDS             100..117
                     /label=His6-tag
     CDS             127..144
                     /label=Thrombin site
     CDS             148..483
                     /label=EutS
     misc_feature    599..620
                     /label=CAP binding site
     promoter        635..665
                     /label=Modified Plac Promoter
     RBS             689..696
                     /label=RBS
     CDS             703..993
                     /label=EutM
     CDS             1106..1393
                     /label=EutN
     misc_feature    1509..1530
                     /label=CAP binding site
     promoter        1545..1575
                     /label=Modified Plac Promoter
     misc_feature    1599..1606
                     /label=RBS
     CDS             1613..2272
                     /label=EutL
     CDS             2285..2779
                     /label=EutK
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttccccacta gaaataattt
       61 tgtttaactt taagaaggag atataccatg ggcagcagcc atcatcatca tcatcacagc
      121 agcggcctgg tgccgcgcgg cagccatatg aataaagaac gcattattca ggaatttgtg
      181 ccgggcaaac aggtcacgct ggcgcatctc attgcgcacc ctggtgaaga actggcgaaa
      241 aagatcggcg ttcccgatgc tggcgcgatt ggcattatga ctttaacgcc gggggaaacg
      301 gcgatgatcg ctggcgattt agcgatgaag gctgcggatg tacacatcgg ttttctcgat
      361 agatttagcg gcgcgctggt gatttatggc accgtcggcg cagtagaaga agcgctatta
      421 cagacggtca gcgggctggg gcgattatta aactttaccc tgtgtgagtt aacaaaaagt
      481 taagcggccg cctcgagcat caccatcacc atcactgatt ctccttacgc atctgtgcgg
      541 tatttcacac cgcatgtact agacccgact ggaaagcggg cagtgagcgc aacgcaatta
      601 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
      661 tgttgtgtgg aattgtgagc gtctagtaga aggaggagat ctatggaagc attaggaatg
      721 attgaaaccc ggggcctggt tgcgctgatt gaggcctccg atgcgatggt aaaagccgcg
      781 cgcgtgaagc tggtcggcgt gaagcagatt ggcggtggcc tgtgtactgc catggtgcgt
      841 ggcgatgtgg cggcgtgcaa agccgcaacc gatgctggcg ccgctgcggc gcagcgcatt
      901 ggcgagttgg tctccgtaca cgtgattcca cgcccgcacg gcgatctgga agaagtgttc
      961 ccgatcagct tcaaaggcga cagcaacatt tgatggtagg ccggataaga cgcgcagcgt
     1021 cgccatccgg catgaatgcg cgccattgcc tgatggtgcg cagattcaag gcctacgacc
     1081 caacgggcaa tccatggagg cggatatgaa actggcagtc gtcacaggac aaatcgtatg
     1141 taccgtccgc catcagggac tggcgcatga caaattgctg atggtggaaa tgatcgatgc
     1201 ccagggcaac cccgacgggc agtgtgccgt cgctatcgac agtatcgggg cgggaaccgg
     1261 agagtgggta ctgctggtca gcggcagttc cgcacgccag gcgcatcgca gcgaattatc
     1321 accggtcgat ctgtgcgtca ttggcatcgt cgatgaagtg gtggctggcg ggaaagtggt
     1381 tttccataaa taggcggccg cctcgagcat caccatcacc atcactgatt ctccttacgc
     1441 atctgtgcgg tatttcacac cgcatgtact agacccgact ggaaagcggg cagtgagcgc
     1501 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     1561 ccggctcgta tgttgtgtgg aattgtgagc gtctagtaga aggaggagat ctatgcctgc
     1621 attagattta attcgacctt cagtgactgc catgcgcgtg attgcctccg tgaatgacgg
     1681 ctttgcgcgg gaacttaaat taccgccaca tatacgtagt ctcggactca tcacggcaga
     1741 ttctgatgat gtgacctata ttgccgctga cgaagcgaca aaacaggcga tggtggaagt
     1801 ggtctatggc cgctccctct atgccggggc ggctcacggg ccgtcgccta ccgctggtga
     1861 ggtgttgatt atgctgggcg gaccgaaccc ggcggaagtg cgcgccggtc tggatgcaat
     1921 ggtcgccagc attgaaaacg gcgcggcgtt ccagtgggcg aacgatgcgg aaaataccgc
     1981 attcctggcg catgtggttt cgcgtaccgg ctcgtacctc tcgtctaccg caggtatcgc
     2041 gctgggcgat ccaatggcct acctggtggc gccgccgctg gaagcgacat tcggcattga
     2101 cgcggcgatg aaatccgctg acgttcagct ggtgacttac gtgccgccgc cgtcggaaac
     2161 caactattcg gccgcgttct taacgggaag ccaggccgcg tgtaaagccg cctgcaacgc
     2221 ctttaccgac gccgtgctgg atatcgcccg taaccctgtc cagcgtgcgt aacggaggct
     2281 gccaatgatc aatgccctgg gattactgga agtggacgga atggtcgccg ccgtcgatgc
     2341 ggcggatgct atgctgaaag cggccaacgt gcgtttgctc agtcatcaag tgctcgaccc
     2401 tggcaggctc acgctggtgg tggagggcga tctggcggcg tgtcgtgcgg cgctggatgc
     2461 cggaagcgct gccgcgcagc gcacaggccg tgtgattagt cgcaaagaaa ttggtcgtcc
     2521 ggaagaggac acccagtggc tgattggcgg gtttgcgcgt gcgacaacgc cgactgagaa
     2581 agcaccccag gtacccgcaa cgcctgagtt cgctgaggcg ttattggcgc tgctggcatc
     2641 agtacgccag ggaatgacgg cgggagaggt tgccgcgcat tttggctggc cgctggagca
     2701 ggccagaaac gtgctggaac aactcttttc tgacggagcg ttgcgtaaac gcagtagtcg
     2761 ctatcgcatc aaaaattaa
//