LOCUS BBa_K1428001 2779 bp DNA linear 22-Dec-2024 DEFINITION Gene cluster for Expressing a purifiable Eut Bacterial Microcompartment ACCESSION BBa_K1428001 VERSION BBa_K1428001.1 FEATURES Location/Qualifiers promoter 1..19 /label=T7 Promoter misc_feature 20..44 /label=lac operator RBS 64..80 /label=T7 RBS misc_feature 88..90 /label=Start codon CDS 100..117 /label=His6-tag CDS 127..144 /label=Thrombin site CDS 148..483 /label=EutS misc_feature 599..620 /label=CAP binding site promoter 635..665 /label=Modified Plac Promoter RBS 689..696 /label=RBS CDS 703..993 /label=EutM CDS 1106..1393 /label=EutN misc_feature 1509..1530 /label=CAP binding site promoter 1545..1575 /label=Modified Plac Promoter misc_feature 1599..1606 /label=RBS CDS 1613..2272 /label=EutL CDS 2285..2779 /label=EutK ORIGIN 1 taatacgact cactataggg gaattgtgag cggataacaa ttccccacta gaaataattt 61 tgtttaactt taagaaggag atataccatg ggcagcagcc atcatcatca tcatcacagc 121 agcggcctgg tgccgcgcgg cagccatatg aataaagaac gcattattca ggaatttgtg 181 ccgggcaaac aggtcacgct ggcgcatctc attgcgcacc ctggtgaaga actggcgaaa 241 aagatcggcg ttcccgatgc tggcgcgatt ggcattatga ctttaacgcc gggggaaacg 301 gcgatgatcg ctggcgattt agcgatgaag gctgcggatg tacacatcgg ttttctcgat 361 agatttagcg gcgcgctggt gatttatggc accgtcggcg cagtagaaga agcgctatta 421 cagacggtca gcgggctggg gcgattatta aactttaccc tgtgtgagtt aacaaaaagt 481 taagcggccg cctcgagcat caccatcacc atcactgatt ctccttacgc atctgtgcgg 541 tatttcacac cgcatgtact agacccgact ggaaagcggg cagtgagcgc aacgcaatta 601 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 661 tgttgtgtgg aattgtgagc gtctagtaga aggaggagat ctatggaagc attaggaatg 721 attgaaaccc ggggcctggt tgcgctgatt gaggcctccg atgcgatggt aaaagccgcg 781 cgcgtgaagc tggtcggcgt gaagcagatt ggcggtggcc tgtgtactgc catggtgcgt 841 ggcgatgtgg cggcgtgcaa agccgcaacc gatgctggcg ccgctgcggc gcagcgcatt 901 ggcgagttgg tctccgtaca cgtgattcca cgcccgcacg gcgatctgga agaagtgttc 961 ccgatcagct tcaaaggcga cagcaacatt tgatggtagg ccggataaga cgcgcagcgt 1021 cgccatccgg catgaatgcg cgccattgcc tgatggtgcg cagattcaag gcctacgacc 1081 caacgggcaa tccatggagg cggatatgaa actggcagtc gtcacaggac aaatcgtatg 1141 taccgtccgc catcagggac tggcgcatga caaattgctg atggtggaaa tgatcgatgc 1201 ccagggcaac cccgacgggc agtgtgccgt cgctatcgac agtatcgggg cgggaaccgg 1261 agagtgggta ctgctggtca gcggcagttc cgcacgccag gcgcatcgca gcgaattatc 1321 accggtcgat ctgtgcgtca ttggcatcgt cgatgaagtg gtggctggcg ggaaagtggt 1381 tttccataaa taggcggccg cctcgagcat caccatcacc atcactgatt ctccttacgc 1441 atctgtgcgg tatttcacac cgcatgtact agacccgact ggaaagcggg cagtgagcgc 1501 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt 1561 ccggctcgta tgttgtgtgg aattgtgagc gtctagtaga aggaggagat ctatgcctgc 1621 attagattta attcgacctt cagtgactgc catgcgcgtg attgcctccg tgaatgacgg 1681 ctttgcgcgg gaacttaaat taccgccaca tatacgtagt ctcggactca tcacggcaga 1741 ttctgatgat gtgacctata ttgccgctga cgaagcgaca aaacaggcga tggtggaagt 1801 ggtctatggc cgctccctct atgccggggc ggctcacggg ccgtcgccta ccgctggtga 1861 ggtgttgatt atgctgggcg gaccgaaccc ggcggaagtg cgcgccggtc tggatgcaat 1921 ggtcgccagc attgaaaacg gcgcggcgtt ccagtgggcg aacgatgcgg aaaataccgc 1981 attcctggcg catgtggttt cgcgtaccgg ctcgtacctc tcgtctaccg caggtatcgc 2041 gctgggcgat ccaatggcct acctggtggc gccgccgctg gaagcgacat tcggcattga 2101 cgcggcgatg aaatccgctg acgttcagct ggtgacttac gtgccgccgc cgtcggaaac 2161 caactattcg gccgcgttct taacgggaag ccaggccgcg tgtaaagccg cctgcaacgc 2221 ctttaccgac gccgtgctgg atatcgcccg taaccctgtc cagcgtgcgt aacggaggct 2281 gccaatgatc aatgccctgg gattactgga agtggacgga atggtcgccg ccgtcgatgc 2341 ggcggatgct atgctgaaag cggccaacgt gcgtttgctc agtcatcaag tgctcgaccc 2401 tggcaggctc acgctggtgg tggagggcga tctggcggcg tgtcgtgcgg cgctggatgc 2461 cggaagcgct gccgcgcagc gcacaggccg tgtgattagt cgcaaagaaa ttggtcgtcc 2521 ggaagaggac acccagtggc tgattggcgg gtttgcgcgt gcgacaacgc cgactgagaa 2581 agcaccccag gtacccgcaa cgcctgagtt cgctgaggcg ttattggcgc tgctggcatc 2641 agtacgccag ggaatgacgg cgggagaggt tgccgcgcat tttggctggc cgctggagca 2701 ggccagaaac gtgctggaac aactcttttc tgacggagcg ttgcgtaaac gcagtagtcg 2761 ctatcgcatc aaaaattaa //