BBa_K1428001 1 BBa_K1428001 Gene cluster for Expressing a purifiable Eut Bacterial Microcompartment 2014-11-02T12:00:00Z 2015-05-08T01:10:23Z BBa_K311004, pET28a The following features of the gene cluster are listed from upstream to downstream: A T7 promoter, a T7 RBS, His6-tagged EutS, a Plac promoter, an RBS (B0034), EutM, an RBS (B0034), EutN, a Plac promoter, an RBS (B0034), EutL, an RBS (B0034), EutK. The whole gene cluster encodes five proteins, EutS, EutM, EutN, EutL, EutK, arranged in a specific order such that when induced with 0.2mM IPTG or allowed for leaky expression at 30C overnight, these 5 gene products will be expressed in a specific molar ratio so that they can assemble into the intact organelle of the bacterial microcompartment (BMC) of the Ethanolamine-utilization type (Eut). It was derived from BBa_K311004 and the pET28a vector in 2 cloning steps. If the whole part is put into a bacterial, one can readily express out a whole organelle in the form of BMC for compartmentalization, which, when combined with our second Biobrick BBa_K1428000, can target any coding Biobrick inside the BMC for both in vivo and in vitro applications, for example, metabolic engineering to avoid toxic intermediate damage to the cell, bioremediation for pollutant collection, drug delivery systems by storing nucleic acids, peptides and proteins for replacement therapy, or even virology model studies as the whole thing resembles a viral capsid. false false _1806_ 0 20650 9 Not in stock false Careful considerations about the linker peptide between the His6-tag and EutS must be considered to avoid (1) out-frame fusion and (2) linker peptides which are hydrophobic and inflexible. false Lai Hei Ming annotation2429976 1 Modified Plac Promoter range2429976 1 635 665 annotation2429968 1 T7 Promoter range2429968 1 1 19 annotation2429979 1 EutN range2429979 1 1106 1393 annotation2429971 1 Start codon range2429971 1 88 90 annotation2429972 1 His6-tag range2429972 1 100 117 annotation2429970 1 T7 RBS range2429970 1 64 80 annotation2429978 1 EutM range2429978 1 703 993 annotation2429980 1 CAP binding site range2429980 1 1509 1530 annotation2429969 1 lac operator range2429969 1 20 44 annotation2429974 1 EutS range2429974 1 148 483 annotation2429973 1 Thrombin site range2429973 1 127 144 annotation2429984 1 EutK range2429984 1 2285 2779 annotation2429977 1 RBS range2429977 1 689 696 annotation2429981 1 Modified Plac Promoter range2429981 1 1545 1575 annotation2429975 1 CAP binding site range2429975 1 599 620 annotation2429982 1 RBS range2429982 1 1599 1606 annotation2429983 1 EutL range2429983 1 1613 2272 BBa_K1428001_sequence 1 taatacgactcactataggggaattgtgagcggataacaattccccactagaaataattttgtttaactttaagaaggagatataccatgggcagcagccatcatcatcatcatcacagcagcggcctggtgccgcgcggcagccatatgaataaagaacgcattattcaggaatttgtgccgggcaaacaggtcacgctggcgcatctcattgcgcaccctggtgaagaactggcgaaaaagatcggcgttcccgatgctggcgcgattggcattatgactttaacgccgggggaaacggcgatgatcgctggcgatttagcgatgaaggctgcggatgtacacatcggttttctcgatagatttagcggcgcgctggtgatttatggcaccgtcggcgcagtagaagaagcgctattacagacggtcagcgggctggggcgattattaaactttaccctgtgtgagttaacaaaaagttaagcggccgcctcgagcatcaccatcaccatcactgattctccttacgcatctgtgcggtatttcacaccgcatgtactagacccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgtctagtagaaggaggagatctatggaagcattaggaatgattgaaacccggggcctggttgcgctgattgaggcctccgatgcgatggtaaaagccgcgcgcgtgaagctggtcggcgtgaagcagattggcggtggcctgtgtactgccatggtgcgtggcgatgtggcggcgtgcaaagccgcaaccgatgctggcgccgctgcggcgcagcgcattggcgagttggtctccgtacacgtgattccacgcccgcacggcgatctggaagaagtgttcccgatcagcttcaaaggcgacagcaacatttgatggtaggccggataagacgcgcagcgtcgccatccggcatgaatgcgcgccattgcctgatggtgcgcagattcaaggcctacgacccaacgggcaatccatggaggcggatatgaaactggcagtcgtcacaggacaaatcgtatgtaccgtccgccatcagggactggcgcatgacaaattgctgatggtggaaatgatcgatgcccagggcaaccccgacgggcagtgtgccgtcgctatcgacagtatcggggcgggaaccggagagtgggtactgctggtcagcggcagttccgcacgccaggcgcatcgcagcgaattatcaccggtcgatctgtgcgtcattggcatcgtcgatgaagtggtggctggcgggaaagtggttttccataaataggcggccgcctcgagcatcaccatcaccatcactgattctccttacgcatctgtgcggtatttcacaccgcatgtactagacccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgtctagtagaaggaggagatctatgcctgcattagatttaattcgaccttcagtgactgccatgcgcgtgattgcctccgtgaatgacggctttgcgcgggaacttaaattaccgccacatatacgtagtctcggactcatcacggcagattctgatgatgtgacctatattgccgctgacgaagcgacaaaacaggcgatggtggaagtggtctatggccgctccctctatgccggggcggctcacgggccgtcgcctaccgctggtgaggtgttgattatgctgggcggaccgaacccggcggaagtgcgcgccggtctggatgcaatggtcgccagcattgaaaacggcgcggcgttccagtgggcgaacgatgcggaaaataccgcattcctggcgcatgtggtttcgcgtaccggctcgtacctctcgtctaccgcaggtatcgcgctgggcgatccaatggcctacctggtggcgccgccgctggaagcgacattcggcattgacgcggcgatgaaatccgctgacgttcagctggtgacttacgtgccgccgccgtcggaaaccaactattcggccgcgttcttaacgggaagccaggccgcgtgtaaagccgcctgcaacgcctttaccgacgccgtgctggatatcgcccgtaaccctgtccagcgtgcgtaacggaggctgccaatgatcaatgccctgggattactggaagtggacggaatggtcgccgccgtcgatgcggcggatgctatgctgaaagcggccaacgtgcgtttgctcagtcatcaagtgctcgaccctggcaggctcacgctggtggtggagggcgatctggcggcgtgtcgtgcggcgctggatgccggaagcgctgccgcgcagcgcacaggccgtgtgattagtcgcaaagaaattggtcgtccggaagaggacacccagtggctgattggcgggtttgcgcgtgcgacaacgccgactgagaaagcaccccaggtacccgcaacgcctgagttcgctgaggcgttattggcgctgctggcatcagtacgccagggaatgacggcgggagaggttgccgcgcattttggctggccgctggagcaggccagaaacgtgctggaacaactcttttctgacggagcgttgcgtaaacgcagtagtcgctatcgcatcaaaaattaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z