BBa_K1430003 1 BBa_K1430003 yeast TRP1 gene 2014-09-29T11:00:00Z 2015-05-08T01:10:23Z genomic DNA of S. cerevisiae Selective marker for auxotrophic strains of yeast after transforamtion with plasmid that contains this gene. What does the marker help synthesize? L-tryptphane Where it can be used? In TRP1 deficient strains of S. cerevisiae false false _1808_ 0 20159 9 It's complicated false We mutated the restriction site for Xba1 in the sequence that is gets compatibel to Prefix/Suffix enzymes. We used PCR approch with primers that add a different base in the recognition site false Cedric Bl??tz annotation2390800 1 s_mutation range2390800 1 87 87 annotation2390799 1 TRP1 range2390799 1 1 675 BBa_K1430003_sequence 1 atgtctgttattaatttcacaggtagttctggtccattggtgaaagtttgcggcttgcagagcacagaggccgcagaatgtgctctggattccgatgctgacttgctgggtattatatgtgtgcccaatagaaagagaacaattgacccggttattgcaaggaaaatttcaagtcttgtaaaagcatataaaaatagttcaggcactccgaaatacttggttggcgtgtttcgtaatcaacctaaggaggatgttttggctctggtcaatgattacggcattgatatcgtccaactgcatggagatgagtcgtggcaagaataccaagagttcctcggtttgccagttattaaaagactcgtatttccaaaagactgcaacatactactcagtgcagcttcacagaaacctcattcgtttattcccttgtttgattcagaagcaggtgggacaggtgaacttttggattggaactcgatttctgactgggttggaaggcaagagagccccgaaagcttacattttatgttagctggtggactgacgccagaaaatgttggtgatgcgcttagattaaatggcgttattggtgttgatgtaagcggaggtgtggagacaaatggtgtaaaagactctaacaaaatagcaaatttcgtcaaaaatgctaagaaatag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z