LOCUS       BBa_K1430005            2953 bp    DNA     linear       25-Nov-2024
DEFINITION  GFP tag for yeast proteins
ACCESSION   BBa_K1430005
VERSION     BBa_K1430005.1  
FEATURES             Location/Qualifiers
     promoter        1..500
                     /label=TDH3 Promoter
     CDS             501..1176
                     /label=TRP1
     misc_feature    587..587
                     /label=deletion of Xba1
     misc_feature    1177..1489
                     /label=HHO1 UTR
     misc_feature    1893..1899
                     /label=BglII
     misc_feature    1902..1908
                     /label=BamHI
     CDS             1910..2626
                     /label=GFP
     misc_feature    2627..2953
                     /label=ADH1-Terminator
     promoter        1490..1890
                     /label=TEF2 Promoter
ORIGIN
        1 acagtttatt cctggcatcc actaaatata atggagcccg ctttttaagc tggcatccag
       61 aaaaaaaaag aatcccagca ccaaaatatt gttttcttca ccaaccatca gttcataggt
      121 ccattctctt agcgcaacta cagagaacag gggcacaaac aggcaaaaaa cgggcacaac
      181 ctcaatggag tgatgcaacc tgcctggagt aaatgatgac acaaggcaat tgacccacgc
      241 atgtatctat ctcattttct tacaccttct attaccttct gctctctctg atttggaaaa
      301 agctgaaaaa aaaggttgaa accagttccc tgaaattatt cccctacttg actaataagt
      361 atataaagac ggtaggtatt gattgtaatt ctgtaaatct atttcttaaa cttcttaaat
      421 tctactttta tagttagtct tttttttagt tttaaaacac caagaactta gtttcgaata
      481 aacacacata aacaaacaaa atgtctgtta ttaatttcac aggtagttct ggtccattgg
      541 tgaaagtttg cggcttgcag agcacagagg ccgcagaatg tgctctggat tccgatgctg
      601 acttgctggg tattatatgt gtgcccaata gaaagagaac aattgacccg gttattgcaa
      661 ggaaaatttc aagtcttgta aaagcatata aaaatagttc aggcactccg aaatacttgg
      721 ttggcgtgtt tcgtaatcaa cctaaggagg atgttttggc tctggtcaat gattacggca
      781 ttgatatcgt ccaactgcat ggagatgagt cgtggcaaga ataccaagag ttcctcggtt
      841 tgccagttat taaaagactc gtatttccaa aagactgcaa catactactc agtgcagctt
      901 cacagaaacc tcattcgttt attcccttgt ttgattcaga agcaggtggg acaggtgaac
      961 ttttggattg gaactcgatt tctgactggg ttggaaggca agagagcccc gaaagcttac
     1021 attttatgtt agctggtgga ctgacgccag aaaatgttgg tgatgcgctt agattaaatg
     1081 gcgttattgg tgttgatgta agcggaggtg tggagacaaa tggtgtaaaa gactctaaca
     1141 aaatagcaaa tttcgtcaaa aatgctaaga aatagaaagt gaatatccaa acgagaatgt
     1201 caatggtgat agcaatacta tcaaacctat tttctttctt catttttgtg tcccatcact
     1261 attagtattt tgtattattt ctaaattttt ttctctctaa ttttcaatct tattcaagtg
     1321 tttgttggcg gtactgtaaa ttttcatttt ttaatctctc ccttctctat ttgttactta
     1381 ttcggtaaat atctccttta tgattttatt caaattccat tcttaactaa ctaactatac
     1441 tgtttataaa tttctcttct cttatatatg caaggtacag tgtgtaaatt acccataagg
     1501 ttgtttgtga cggcgtcgta caagagaacg tgggaacttt ttaggctcac caaaaaagaa
     1561 agaaaaaata cgagttgctg acagaagcct caagaaaaaa aaaattcttc ttcgactatg
     1621 ctggaggcag agatgatcga gccggtagtt aactatatat agctaaattg gttccatcac
     1681 cttcttttct ggtgtcgctc cttctagtgc tatttctggc ttttcctatt tttttttttc
     1741 catttttctt tctctctttc taatatataa attctcttgc attttctatt tttctctcta
     1801 tctattctac ttgtttattc ccttcaaggt ttttttttaa ggagtacttg tttttagaat
     1861 atacggtcaa cgaactataa ttaactaaac gaagatcttc cggatccgga tgcgtaaagg
     1921 agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg
     1981 gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct
     2041 taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt
     2101 cggttatggt gttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt
     2161 caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg
     2221 gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga
     2281 gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa
     2341 ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa
     2401 cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca
     2461 aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca
     2521 atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt
     2581 aacagctgct gggattacac atggcatgga tgaactatac aaataataaa gctttggact
     2641 tcttcgccag aggtttggtc aagtctccaa tcaaggttgt cggcttgtct accttgccag
     2701 aaatttacga aaagatggaa aagggtcaaa tcgttggtag atacgttgtt gacacttcta
     2761 aataagcgaa tttcttatga tttatgattt ttattattaa ataagttata aaaaaaataa
     2821 gtgtatacaa attttaaagt gactcttagg ttttaaaacg aaaattctta ttcttgagta
     2881 actctttcct gtaggtcagg ttgctttctc aggtatagca tgaggtcgct cttattgacc
     2941 acacctctac cgg
//