LOCUS       BBa_K1430006            3334 bp    DNA     linear       01-Jun-2024
DEFINITION  mRFP tag for yeast proteins
ACCESSION   BBa_K1430006
VERSION     BBa_K1430006.1  
FEATURES             Location/Qualifiers
     misc_feature    3008..3334
                     /label=ADH1-Terminator
     CDS             2330..3007
                     /label=mRFP
     misc_feature    2322..2328
                     /label=BamH1
     misc_feature    2313..2319
                     /label=BglII
     promoter        1..500
                     /label=TDH3 Promoter
     CDS             501..1595
                     /label=LEU2 
     misc_feature    1596..1907
                     /label=HHO1 Terminator
     promoter        1908..2310
                     /label=TEF2 Promoter
ORIGIN
        1 acagtttatt cctggcatcc actaaatata atggagcccg ctttttaagc tggcatccag
       61 aaaaaaaaag aatcccagca ccaaaatatt gttttcttca ccaaccatca gttcataggt
      121 ccattctctt agcgcaacta cagagaacag gggcacaaac aggcaaaaaa cgggcacaac
      181 ctcaatggag tgatgcaacc tgcctggagt aaatgatgac acaaggcaat tgacccacgc
      241 atgtatctat ctcattttct tacaccttct attaccttct gctctctctg atttggaaaa
      301 agctgaaaaa aaaggttgaa accagttccc tgaaattatt cccctacttg actaataagt
      361 atataaagac ggtaggtatt gattgtaatt ctgtaaatct atttcttaaa cttcttaaat
      421 tctactttta tagttagtct tttttttagt tttaaaacac caagaactta gtttcgaata
      481 aacacacata aacaaacaaa atgtctgccc ctaagaagat cgtcgttttg ccaggtgacc
      541 acgttggtca agaaatcaca gccgaagcca ttaaggttct taaagctatt tctgatgttc
      601 gttccaatgt caagttcgat ttcgaaaatc atttaattgg tggtgctgct atcgatgcta
      661 caggtgtccc acttccagat gaggcgctgg aagcctccaa gaaggttgat gccgttttgt
      721 taggtgctgt gggtggtcct aaatggggta ccggtagtgt tagacctgaa caaggtttac
      781 taaaaatccg taaagaactt caattgtacg ccaacttaag accatgtaac tttgcatccg
      841 actctctttt agacttatct ccaatcaagc cacaatttgc taaaggtact gacttcgttg
      901 ttgtcagaga attagtggga ggtatttact ttggtaagag aaaggaagac gatggtgatg
      961 gtgtcgcttg ggatagtgaa caatacaccg ttccagaagt gcaaagaatc acaagaatgg
     1021 ccgctttcat ggccctacaa catgagccac cattgcctat ttggtccttg gataaagcta
     1081 atgttttggc ctcttcaaga ttatggagaa aaactgtgga ggaaaccatc aagaacgagt
     1141 tccctacatt gaaggttcaa catcaattga ttgattctgc cgccatgatc ctagttaaga
     1201 acccaaccca cctaaatggt attataatca ccagcaacat gtttggtgat atcatctccg
     1261 atgaagcctc cgttatccca ggttccttgg gtttgttgcc atctgcgtcc ttggcctctt
     1321 tgccagacaa gaacaccgca tttggtttgt acgaaccatg ccacggttct gctccagatt
     1381 tgccaaagaa taaggttgac cctatcgcca ctatcttgtc tgctgcaatg atgttgaaat
     1441 tgtcattgaa cttgcctgaa gaaggtaagg ccattgaaga tgcagttaaa aaggttttgg
     1501 atgcaggtat cagaactggt gatttaggtg gttccaacag taccaccgaa gtcggtgatg
     1561 ctgtcgccga agaagttaag aaaatccttg cttaaaaagt gaatatccaa acgagaatgt
     1621 caatggtgat agcaatacta tcaaacctat tttctttctt catttttgtg tcccatcact
     1681 attagtattt tgtattattt ctaaattttt ttctctctaa ttttcaatct tattcaagtg
     1741 tttgttggcg gtactgtaaa ttttcatttt ttaatctctc ccttctctat ttgttactta
     1801 ttcggtaaat atctccttta tgattttatt caaattccat tcttaactaa ctaactatac
     1861 tgtttataaa tttctcttct cttatatatg caaggtacag tgtgtaaatt acccataagg
     1921 ttgtttgtga cggcgtcgta caagagaacg tgggaacttt ttaggctcac caaaaaagaa
     1981 agaaaaaata cgagttgctg acagaagcct caagaaaaaa aaaattcttc ttcgactatg
     2041 ctggaggcag agatgatcga gccggtagtt aactatatat agctaaattg gttccatcac
     2101 cttcttttct ggtgtcgctc cttctagtgc tatttctggc ttttcctatt tttttttttc
     2161 catttttctt tctctctttc taatatataa attctcttgc attttctatt tttctctcta
     2221 tctattctac ttgtttattc ccttcaaggt ttttttttaa ggagtacttg tttttagaat
     2281 atacggtcaa cgaactataa ttaactaaac gaagatcttc cggatccgga tggcttcctc
     2341 cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg
     2401 tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc
     2461 taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca
     2521 gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa
     2581 actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt
     2641 tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact
     2701 gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga
     2761 agcttccacc gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg
     2821 tctgaaactg aaagacggtg gtcactacga cgctgaagtt aaaaccacct acatggctaa
     2881 aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca
     2941 caacgaagac tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg
     3001 tgcttaataa agctttggac ttcttcgcca gaggtttggt caagtctcca atcaaggttg
     3061 tcggcttgtc taccttgcca gaaatttacg aaaagatgga aaagggtcaa atcgttggta
     3121 gatacgttgt tgacacttct aaataagcga atttcttatg atttatgatt tttattatta
     3181 aataagttat aaaaaaaata agtgtataca aattttaaag tgactcttag gttttaaaac
     3241 gaaaattctt attcttgagt aactctttcc tgtaggtcag gttgctttct caggtatagc
     3301 atgaggtcgc tcttattgac cacacctcta ccgg
//