LOCUS BBa_K1430007 4304 bp DNA linear 22-Nov-2024 DEFINITION Tool to tag yeast proteins with GFP ACCESSION BBa_K1430007 VERSION BBa_K1430007.1 FEATURES Location/Qualifiers promoter 1..500 /label=TDH3 Promoter CDS 501..1176 /label=TRP1 misc_feature 587..587 /label=deletion of Xba1 misc_feature 1177..1489 /label=HHO1 UTR misc_feature 1893..1899 /label=BglII misc_feature 1902..1908 /label=BamHI CDS 1910..2626 /label=GFP misc_feature 2627..2953 /label=ADH1-Terminator promoter 1490..1890 /label=TEF2 Promoter misc_feature 1..2953 /label=BBa_K1430005 misc_feature 2962..4304 /label=2-micron ori promoter 2962..4304 /label=BBa_K1430000 ORIGIN 1 acagtttatt cctggcatcc actaaatata atggagcccg ctttttaagc tggcatccag 61 aaaaaaaaag aatcccagca ccaaaatatt gttttcttca ccaaccatca gttcataggt 121 ccattctctt agcgcaacta cagagaacag gggcacaaac aggcaaaaaa cgggcacaac 181 ctcaatggag tgatgcaacc tgcctggagt aaatgatgac acaaggcaat tgacccacgc 241 atgtatctat ctcattttct tacaccttct attaccttct gctctctctg atttggaaaa 301 agctgaaaaa aaaggttgaa accagttccc tgaaattatt cccctacttg actaataagt 361 atataaagac ggtaggtatt gattgtaatt ctgtaaatct atttcttaaa cttcttaaat 421 tctactttta tagttagtct tttttttagt tttaaaacac caagaactta gtttcgaata 481 aacacacata aacaaacaaa atgtctgtta ttaatttcac aggtagttct ggtccattgg 541 tgaaagtttg cggcttgcag agcacagagg ccgcagaatg tgctctggat tccgatgctg 601 acttgctggg tattatatgt gtgcccaata gaaagagaac aattgacccg gttattgcaa 661 ggaaaatttc aagtcttgta aaagcatata aaaatagttc aggcactccg aaatacttgg 721 ttggcgtgtt tcgtaatcaa cctaaggagg atgttttggc tctggtcaat gattacggca 781 ttgatatcgt ccaactgcat ggagatgagt cgtggcaaga ataccaagag ttcctcggtt 841 tgccagttat taaaagactc gtatttccaa aagactgcaa catactactc agtgcagctt 901 cacagaaacc tcattcgttt attcccttgt ttgattcaga agcaggtggg acaggtgaac 961 ttttggattg gaactcgatt tctgactggg ttggaaggca agagagcccc gaaagcttac 1021 attttatgtt agctggtgga ctgacgccag aaaatgttgg tgatgcgctt agattaaatg 1081 gcgttattgg tgttgatgta agcggaggtg tggagacaaa tggtgtaaaa gactctaaca 1141 aaatagcaaa tttcgtcaaa aatgctaaga aatagaaagt gaatatccaa acgagaatgt 1201 caatggtgat agcaatacta tcaaacctat tttctttctt catttttgtg tcccatcact 1261 attagtattt tgtattattt ctaaattttt ttctctctaa ttttcaatct tattcaagtg 1321 tttgttggcg gtactgtaaa ttttcatttt ttaatctctc ccttctctat ttgttactta 1381 ttcggtaaat atctccttta tgattttatt caaattccat tcttaactaa ctaactatac 1441 tgtttataaa tttctcttct cttatatatg caaggtacag tgtgtaaatt acccataagg 1501 ttgtttgtga cggcgtcgta caagagaacg tgggaacttt ttaggctcac caaaaaagaa 1561 agaaaaaata cgagttgctg acagaagcct caagaaaaaa aaaattcttc ttcgactatg 1621 ctggaggcag agatgatcga gccggtagtt aactatatat agctaaattg gttccatcac 1681 cttcttttct ggtgtcgctc cttctagtgc tatttctggc ttttcctatt tttttttttc 1741 catttttctt tctctctttc taatatataa attctcttgc attttctatt tttctctcta 1801 tctattctac ttgtttattc ccttcaaggt ttttttttaa ggagtacttg tttttagaat 1861 atacggtcaa cgaactataa ttaactaaac gaagatcttc cggatccgga tgcgtaaagg 1921 agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg 1981 gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct 2041 taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt 2101 cggttatggt gttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt 2161 caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg 2221 gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga 2281 gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa 2341 ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa 2401 cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca 2461 aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca 2521 atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt 2581 aacagctgct gggattacac atggcatgga tgaactatac aaataataaa gctttggact 2641 tcttcgccag aggtttggtc aagtctccaa tcaaggttgt cggcttgtct accttgccag 2701 aaatttacga aaagatggaa aagggtcaaa tcgttggtag atacgttgtt gacacttcta 2761 aataagcgaa tttcttatga tttatgattt ttattattaa ataagttata aaaaaaataa 2821 gtgtatacaa attttaaagt gactcttagg ttttaaaacg aaaattctta ttcttgagta 2881 actctttcct gtaggtcagg ttgctttctc aggtatagca tgaggtcgct cttattgacc 2941 acacctctac cggtactaga ggatccaata tcaaaggaaa tgatagcatt gaaggatgag 3001 actaatccaa ttgaggagtg gcagcatata gaacagctaa agggtagtgc tgaaggaagc 3061 atacgatacc ccgcatggaa tgggataata tcacaggagg tactagacta cctttcatcc 3121 tacataaata gacgcatata agtacgcatt taagcataaa cacgcactat gccgttcttc 3181 tcatgtatat atatatacag gcaacacgca gatataggtg cgacgtgaac agtgagctgt 3241 atgtgcgcag ctcgcgttgc attttcggaa gcgctcgttt tcggaaacgc tttgaagttc 3301 ctattccgaa gttcctattc tctacaaagt ataggaactt cagagcgctt ttgaaaacca 3361 aaagcgctct gaagacgcac tttcaaaaaa ccaaaaacgc accggactgt aacgagctac 3421 taaaatattg cgaataccgc ttccacaaac attgctcaaa agtatctctt tgctatatat 3481 ctctgtgcta tatccctata taacctaccc atccaccttt cgctccttga acttgcatct 3541 aaactcgacc tctacatttt ttatgtttat ctctagtatt actctttaga caaaaaaatt 3601 gtagtaagaa ctattcatag agtgaatcga aaacaatacg aaaatgtaaa catttcctat 3661 acgtagtata tagagacaaa atagaagaaa ccgttcataa ttttctgacc aatgaagaat 3721 catcaacgct atcactttct gttcacaaag tatgcgcaat ccacatcggt atagaatata 3781 atcggggatg cctttatctt gaaaaaatgc acccgcagct tcgctagtaa tcagtaaacg 3841 cgggaagtgg agtcaggctt tttttatgga agagaaaata gacaccaaag tagccttctt 3901 ctaaccttaa cggacctaca gtgcaaaaag ttatcaagag actgcattat agagcgcaca 3961 aaggagaaaa aaagtaatct aagatgcttt gttagaaaaa tagcgctctc gggatgcatt 4021 tttgtagaac aaaaaagaag tatagattct ttgttggtaa aatagcgctc tcgcgttgca 4081 tttctgttct gtaaaaatgc agctcagatt ctttgtttga aaaattagcg ctctcgcgtt 4141 gcatttttgt tttacaaaaa tgaagcacag attcttcgtt ggtaaaatag cgctttcgcg 4201 ttgcatttct gttctgtaaa aatgcagctc agattctttg tttgaaaaat tagcgctctc 4261 gcgttgcatt tttgttctac aaaatgaagc acagatgctt cgtt //