LOCUS BBa_K1430008 4685 bp DNA linear 22-Dec-2024 DEFINITION Tool to tag yeast proteins with mRFP ACCESSION BBa_K1430008 VERSION BBa_K1430008.1 FEATURES Location/Qualifiers misc_feature 3008..3334 /label=ADH1-Terminator CDS 2330..3007 /label=mRFP misc_feature 2322..2328 /label=BamH1 misc_feature 2313..2319 /label=BglII promoter 1..500 /label=TDH3 Promoter CDS 501..1595 /label=LEU2 misc_feature 1596..1907 /label=HHO1 Terminator promoter 1908..2310 /label=TEF2 Promoter misc_feature 1..3334 /label=BBa_K1430006 misc_feature 3343..4685 /label=2-micron ori promoter 3343..4685 /label=BBa_K1430000 ORIGIN 1 acagtttatt cctggcatcc actaaatata atggagcccg ctttttaagc tggcatccag 61 aaaaaaaaag aatcccagca ccaaaatatt gttttcttca ccaaccatca gttcataggt 121 ccattctctt agcgcaacta cagagaacag gggcacaaac aggcaaaaaa cgggcacaac 181 ctcaatggag tgatgcaacc tgcctggagt aaatgatgac acaaggcaat tgacccacgc 241 atgtatctat ctcattttct tacaccttct attaccttct gctctctctg atttggaaaa 301 agctgaaaaa aaaggttgaa accagttccc tgaaattatt cccctacttg actaataagt 361 atataaagac ggtaggtatt gattgtaatt ctgtaaatct atttcttaaa cttcttaaat 421 tctactttta tagttagtct tttttttagt tttaaaacac caagaactta gtttcgaata 481 aacacacata aacaaacaaa atgtctgccc ctaagaagat cgtcgttttg ccaggtgacc 541 acgttggtca agaaatcaca gccgaagcca ttaaggttct taaagctatt tctgatgttc 601 gttccaatgt caagttcgat ttcgaaaatc atttaattgg tggtgctgct atcgatgcta 661 caggtgtccc acttccagat gaggcgctgg aagcctccaa gaaggttgat gccgttttgt 721 taggtgctgt gggtggtcct aaatggggta ccggtagtgt tagacctgaa caaggtttac 781 taaaaatccg taaagaactt caattgtacg ccaacttaag accatgtaac tttgcatccg 841 actctctttt agacttatct ccaatcaagc cacaatttgc taaaggtact gacttcgttg 901 ttgtcagaga attagtggga ggtatttact ttggtaagag aaaggaagac gatggtgatg 961 gtgtcgcttg ggatagtgaa caatacaccg ttccagaagt gcaaagaatc acaagaatgg 1021 ccgctttcat ggccctacaa catgagccac cattgcctat ttggtccttg gataaagcta 1081 atgttttggc ctcttcaaga ttatggagaa aaactgtgga ggaaaccatc aagaacgagt 1141 tccctacatt gaaggttcaa catcaattga ttgattctgc cgccatgatc ctagttaaga 1201 acccaaccca cctaaatggt attataatca ccagcaacat gtttggtgat atcatctccg 1261 atgaagcctc cgttatccca ggttccttgg gtttgttgcc atctgcgtcc ttggcctctt 1321 tgccagacaa gaacaccgca tttggtttgt acgaaccatg ccacggttct gctccagatt 1381 tgccaaagaa taaggttgac cctatcgcca ctatcttgtc tgctgcaatg atgttgaaat 1441 tgtcattgaa cttgcctgaa gaaggtaagg ccattgaaga tgcagttaaa aaggttttgg 1501 atgcaggtat cagaactggt gatttaggtg gttccaacag taccaccgaa gtcggtgatg 1561 ctgtcgccga agaagttaag aaaatccttg cttaaaaagt gaatatccaa acgagaatgt 1621 caatggtgat agcaatacta tcaaacctat tttctttctt catttttgtg tcccatcact 1681 attagtattt tgtattattt ctaaattttt ttctctctaa ttttcaatct tattcaagtg 1741 tttgttggcg gtactgtaaa ttttcatttt ttaatctctc ccttctctat ttgttactta 1801 ttcggtaaat atctccttta tgattttatt caaattccat tcttaactaa ctaactatac 1861 tgtttataaa tttctcttct cttatatatg caaggtacag tgtgtaaatt acccataagg 1921 ttgtttgtga cggcgtcgta caagagaacg tgggaacttt ttaggctcac caaaaaagaa 1981 agaaaaaata cgagttgctg acagaagcct caagaaaaaa aaaattcttc ttcgactatg 2041 ctggaggcag agatgatcga gccggtagtt aactatatat agctaaattg gttccatcac 2101 cttcttttct ggtgtcgctc cttctagtgc tatttctggc ttttcctatt tttttttttc 2161 catttttctt tctctctttc taatatataa attctcttgc attttctatt tttctctcta 2221 tctattctac ttgtttattc ccttcaaggt ttttttttaa ggagtacttg tttttagaat 2281 atacggtcaa cgaactataa ttaactaaac gaagatcttc cggatccgga tggcttcctc 2341 cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg 2401 tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc 2461 taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca 2521 gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa 2581 actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt 2641 tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact 2701 gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga 2761 agcttccacc gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg 2821 tctgaaactg aaagacggtg gtcactacga cgctgaagtt aaaaccacct acatggctaa 2881 aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca 2941 caacgaagac tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg 3001 tgcttaataa agctttggac ttcttcgcca gaggtttggt caagtctcca atcaaggttg 3061 tcggcttgtc taccttgcca gaaatttacg aaaagatgga aaagggtcaa atcgttggta 3121 gatacgttgt tgacacttct aaataagcga atttcttatg atttatgatt tttattatta 3181 aataagttat aaaaaaaata agtgtataca aattttaaag tgactcttag gttttaaaac 3241 gaaaattctt attcttgagt aactctttcc tgtaggtcag gttgctttct caggtatagc 3301 atgaggtcgc tcttattgac cacacctcta ccggtactag aggatccaat atcaaaggaa 3361 atgatagcat tgaaggatga gactaatcca attgaggagt ggcagcatat agaacagcta 3421 aagggtagtg ctgaaggaag catacgatac cccgcatgga atgggataat atcacaggag 3481 gtactagact acctttcatc ctacataaat agacgcatat aagtacgcat ttaagcataa 3541 acacgcacta tgccgttctt ctcatgtata tatatataca ggcaacacgc agatataggt 3601 gcgacgtgaa cagtgagctg tatgtgcgca gctcgcgttg cattttcgga agcgctcgtt 3661 ttcggaaacg ctttgaagtt cctattccga agttcctatt ctctacaaag tataggaact 3721 tcagagcgct tttgaaaacc aaaagcgctc tgaagacgca ctttcaaaaa accaaaaacg 3781 caccggactg taacgagcta ctaaaatatt gcgaataccg cttccacaaa cattgctcaa 3841 aagtatctct ttgctatata tctctgtgct atatccctat ataacctacc catccacctt 3901 tcgctccttg aacttgcatc taaactcgac ctctacattt tttatgttta tctctagtat 3961 tactctttag acaaaaaaat tgtagtaaga actattcata gagtgaatcg aaaacaatac 4021 gaaaatgtaa acatttccta tacgtagtat atagagacaa aatagaagaa accgttcata 4081 attttctgac caatgaagaa tcatcaacgc tatcactttc tgttcacaaa gtatgcgcaa 4141 tccacatcgg tatagaatat aatcggggat gcctttatct tgaaaaaatg cacccgcagc 4201 ttcgctagta atcagtaaac gcgggaagtg gagtcaggct ttttttatgg aagagaaaat 4261 agacaccaaa gtagccttct tctaacctta acggacctac agtgcaaaaa gttatcaaga 4321 gactgcatta tagagcgcac aaaggagaaa aaaagtaatc taagatgctt tgttagaaaa 4381 atagcgctct cgggatgcat ttttgtagaa caaaaaagaa gtatagattc tttgttggta 4441 aaatagcgct ctcgcgttgc atttctgttc tgtaaaaatg cagctcagat tctttgtttg 4501 aaaaattagc gctctcgcgt tgcatttttg ttttacaaaa atgaagcaca gattcttcgt 4561 tggtaaaata gcgctttcgc gttgcatttc tgttctgtaa aaatgcagct cagattcttt 4621 gtttgaaaaa ttagcgctct cgcgttgcat ttttgttcta caaaatgaag cacagatgct 4681 tcgtt //