BBa_K143015
1
Ph-s
Promoter hyper-spank for B. subtilis
2008-09-17T11:00:00Z
2015-05-08T01:10:23Z
The part was designed using the sequence from the ''B.subtilis'' genome and from previously published papers <cite>1</cite><cite>2</cite><cite>3</cite>. This sequence was then synthesised by Geneart.
Promoter hyper-spank is an inducible promoter that has been designed for high expression in ''B.subtilis''. Gene expression under the promoter hyper-spank can be induced by addition of Isopropyl β-D-1-thiogalactopyranoside (IPTG). The context with which we used the promoter hyper-spank, was to take an input of IPTG and give '''Polymerase Per Second'''(PoPS) as an output. IPTG does not induce the promoter hyper-spank directly, but requires the transcriptional regulator '''LacI''', (<bbpart>BBa_K413035</bbpart>). This means that LacI must be constitutively expressed in ''B.subtilis'' in order to use the promoter hyper-spank.
false
false
_199_
0
3475
9
Not in stock
false
Biobrick standard was applied to the promoter hyper-spank sequence.
false
Chris Hirst
annotation1976424
1
Sigma A -35
range1976424
1
46
50
annotation1976423
1
LacI Operator
range1976423
1
10
30
annotation1976425
1
Sigma A -10
range1976425
1
69
74
annotation1976426
1
LacI Operator
range1976426
1
81
101
BBa_K143015_sequence
1
ctcgagggtaaatgtgagcactcacaattcattttgcaaaagttgttgactttatctacaaggtgtggcataatgtgtgtaattgtgagcggataacaatt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z