BBa_K143035
1
LipA-HE
LipA-Human Elastin (EP20-24-24) Fusion Protein
2008-09-17T11:00:00Z
2015-05-08T01:10:24Z
All exons in EP20-24-24 are derived from the human elastin polypeptide gene. EP20-24-24 was used to study the effect of various combinations of exons on coacervation of elastin polypeptide. LipA originated from the B. subtilis genome<cite>4</cite>. Both components were produced as a fusion protein by GeneArt.
Elastin is a polymeric extracellular matrix protein found in tissues that require the ability to extend and recoil. Examples of elastin containing tissues include arteries, lungs, ligaments and skin.
Construct EP20-24-24 for human elastin polypeptide consists of distinct exons which code for alternating hydrophobic regions and crosslinking domains from the human elastin polypeptide gene <cite>1</cite>.
Under appropriate conditions of temperature and ionic strength, elastin polypeptide undergoes a self-aggregation process known as coacervation. Coacervation is usually induced by an increase in temperature and causes the protein to separate from the solution as a second phase. Unlike most proteins which undergo denaturation when the temperature of the solution increases, elastin polypeptides become more ordered through coacervation <cite>2</cite>.
LipA is a signal peptide from the B.subtilis genome. In general, signal peptides are responsible for directing preproteins (secretory proteins with a signal peptide region attached)through an appropriate secretory pathway<cite>3</cite>. LipA has been successfully used in the secretion of heterologous proteins such as cutinase by ''B. subtilis''.
false
false
_199_
0
3429
9
It's complicated
false
BioBrick standard was applied to the LipA-Human Elastin(EP20-24-24) Fusion Protein.
false
Qin Qi
annotation1992710
1
stop
range1992710
1
703
705
annotation1992708
1
Human Elastin (EP20-24-24)
range1992708
1
103
699
annotation1992709
1
stop
range1992709
1
700
702
annotation1976410
1
LipA
range1976410
1
1
102
annotation1992707
1
start
range1992707
1
1
3
BBa_K143035_sequence
1
atgaaatttgtgaaaagacgcattattgcactggttacaattctgatgctgtcagttacatcactgtttgcactgcaaccgtcagcaaaagcagcagaacattttccgggctttggcgttggcgttggaggcattccgggagttgcaggcgttccgggcgttggcggagtcccgggagtcggcggcgttcctggcgtcggcattccggaagcacaagcagcagcagcggcaaaagcagcgaaatatggcgttggcacaccggcagcggcagcagcgaaagcagcagcaaaagcggcacaatttggcctggttccgggcgtcggagttgcaccgggagttggcgttgcgcctggcgttggagtggctccgggagtgggattagcacctggcgtgggcgtagcaccgggtgtgggagttgctcctggtgtcggcgttgctccggcaattggcccggaagcacaggcggctgcggcagcgaaagcggcaaaatatggtgtcggaacacctgcggcagcagcagctaaagctgcggcgaaagcagcgcagtttggccttgtcccgggagttggtgtcgcaccgggcgtaggtgtcgctccgggtgttggagtggcccctggcgtaggccttgctccgggagtaggcgtagctccgggagtcggtgtggcacctggtgtcggcgtcgcaccggcaattggaccgtaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z