BBa_K143038
1
SacB-EAK16
SacB-EAK16-II Fusion Protein
2008-09-17T11:00:00Z
2015-05-08T01:10:24Z
EAK16-II is a segment from zuotin, a yeast protein. SacB was identified from the initial part of certain preprotein genes that utilises the the Sec-SRP secretory pathway <cite>3</cite>. Both components were synthesised as a fusin protein by GeneArt.
EAK16-II is a sixteen amino acid peptide that self-assembles to form β-sheet structures in an aqueous medium. The alternating positive and negative charges (--++--++) are responsible for creating an electrostatic attraction between adjacent peptides <cite>1</cite>, triggering self-assembly when the EAK16-II peptides are exposed to physiological media or salt solution. When examined under SEM, a well-ordered nanofibre structure is formed by the association of the EAK16-II peptides and these nanofibres can futher aggregate to form a membranous 3D scaffold.
SacB is a signal peptide used in the Sec-SRP (secretory signal recognition particle) pathway by ''B. subtilis''. Signal peptides are responsible for directing preproteins (secretory proteins with a signal peptide region attached) through an appropriate secretory pathway. In the case of the Sec-SRP signal peptide, they direct preproteins from the cytoplasm into the growth medium. SacB has been successfully used in the secretion of heterologous proteins such as acid-stable α-amylase, cystatin and interleukin-3 by B.subtilis <cite>2</cite>.
false
false
_199_
0
3429
9
It's complicated
false
BioBrick standard was applied to the SacB-EAK16-II Fusion Protein.
false
Qin Qi
annotation1992726
1
start
range1992726
1
1
3
annotation1992727
1
stop
range1992727
1
145
147
annotation1976411
1
SacB
range1976411
1
1
96
annotation1992725
1
EAK16-II
range1992725
1
97
144
annotation1992728
1
stop
range1992728
1
148
150
BBa_K143038_sequence
1
atgaacatcaaaaaatttgcaaaacaggcgacagttctgacatttacaacagcactgcttgcaggcggcgcaacacaagcatttgcaaaagaaacagcagaagcagaagcgaaagcaaaagctgaagcggaagccaaagcgaaataataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z