BBa_K143039
1
SacB-HE
SacB-Human Elastin (EP20-24-24) Fusion Protein
2008-09-16T11:00:00Z
2015-05-08T01:10:24Z
All exons in EP20-24-24 are derived from the human elastin polypeptide gene. EP20-24-24 was used to study the effect of various combinations of exons on coacervation of elastin polypeptide. SacB was identified from the initial part of certain preprotein genes that utilises the the Sec-SRP secretory pathway <cite>4</cite>. Both components were synthesised as a fusin protein by GeneArt.
Elastin is a polymeric extracellular matrix protein found in tissues that require the ability to extend and recoil. Examples of elastin containing tissues include arteries, lungs, ligaments and skin.
Construct EP20-24-24 for human elastin polypeptide consists of distinct exons which code for alternating hydrophobic regions and crosslinking domains from the human elastin polypeptide gene <cite>1</cite>.
Under appropriate conditions of temperature and ionic strength, elastin polypeptide undergoes a self-aggregation process known as coacervation. Coacervation is usually induced by an increase in temperature and causes the protein to separate from the solution as a second phase. Unlike most proteins which undergo denaturation when the temperature of the solution increases, elastin polypeptides become more ordered through coacervation <cite>2</cite>.
SacB is a signal peptide used in the Sec-SRP (secretory signal recognition particle) pathway by ''B. subtilis''. Signal peptides are responsible for directing preproteins (secretory proteins with a signal peptide region attached) through an appropriate secretory pathway. In the case of the Sec-SRP signal peptide, they direct preproteins from the cytoplasm into the growth medium. SacB has been successfully used in the secretion of heterologous proteins such as acid-stable α-amylase, cystatin and interleukin-3 by B.subtilis <cite>3</cite>.
false
false
_199_
0
3429
9
It's complicated
false
BioBrick standard was applied to the SacB-Human Elastin(EP20-24-24) Fusion Protein.
false
Qin Qi
annotation1992730
1
stop
range1992730
1
697
699
annotation1992729
1
start
range1992729
1
1
3
annotation1992732
1
Human Elastin (EP20-24-24)
range1992732
1
97
693
annotation1992731
1
stop
range1992731
1
694
696
annotation1976019
1
SacB
range1976019
1
1
96
BBa_K143039_sequence
1
atgaacatcaaaaaatttgcaaaacaggcgacagttctgacatttacaacagcactgcttgcaggcggcgcaacacaagcatttgcaaaagaaacatttccgggctttggcgttggcgttggaggcattccgggagttgcaggcgttccgggcgttggcggagtcccgggagtcggcggcgttcctggcgtcggcattccggaagcacaagcagcagcagcggcaaaagcagcaaaatatggcgttggcacaccggcagcggcagcagcgaaagcggcagcaaaagcagcgcaatttggcctggttccgggcgtcggagttgcaccgggagttggcgttgcgcctggcgttggagtggctccgggagtgggattagcacctggcgtgggcgtagcaccgggtgtgggagttgctcctggtgtcggcgttgctccggcaattggcccggaagcacaggcggctgcggcagcgaaagctgcgaaatatggtgtcggaacacctgcggcagcggctgcaaaagctgctgcaaaagcggcacagtttggacttgtcccgggagttggagtcgcaccgggcgtaggtgtcgctccgggtgtaggcgtggcaccgggcgttggattagcgccgggagtcggtgtggcacctggtgtcggagtcgcccctggcgttggtgttgcaccggcgattggaccgtaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z