BBa_K143037 1 YtvA YtvA Blue Light Receptor for B.subtilis 2008-09-14T11:00:00Z 2015-05-08T01:10:24Z The part was designed using the sequence from the B.subtilis genome. This sequence was then synthesised by Geneart. YtvA is a blue light receptor endogenously found in ''B.subtilis''. Activation of the YtvA light receptor activates the mild stress response pathway, accumulating in sigma B activation. Previous research has shown that sigma B dependent transcription in response to blue light light exposure can be enhanced by the over expression of the YtvA receptor <cite>1</cite>. We over expressed YtvA to enhance the transcriptional output of sigma B promoters in response to blue light. false false _199_ 0 2090 9 It's complicated false Biobrick standard was applied to the YtvA sequence. false James Chappell annotation1976412 1 YtvA Blue Light Receptor for B.subtilis range1976412 1 1 783 annotation1992714 1 start range1992714 1 1 3 annotation1992715 1 stop range1992715 1 787 789 annotation1992716 1 stop range1992716 1 784 786 BBa_K143066 1 YtvA-T YtvA light receptor Protein - Terminator 2008-10-25T11:00:00Z 2015-05-08T01:10:24Z YtvA was synthesised by GeneArt and cloned into a BioBrick with the registry double terminator. YtvA light receptor protein (YtvA)(<bbpart>BBa_K143037</bbpart>) coupled to the double terminator (<bbpart>BBa_B0015</bbpart>. YtvA can be used in conjunction with a sigma factor B specific promoter (<bbpart>BBa_K143010</bbpart> or <bbpart>BBa_K143011</bbpart>), where the YtvA will act as a receiver for a blue light input to result in a '''Polymerases per second''' (PoPS) output. The double terminator is the most commonly used terminator and is a combination of parts <bbpart>BBa_B0010</bbpart> and <bbpart>BBa_B0012</bbpart>. The double terminator allows the YtvA to be easily incorporated into a closed transcriptional unit. false false _199_ 0 3475 9 It's complicated false YtvA was identified from the ''B. subtilis'' chromosome. The double terminator is the most commonly used registry terminator. false Chris Hirst component1988420 1 BBa_B0010 component1988419 1 BBa_K143037 component1988422 1 BBa_B0012 annotation1988422 1 BBa_B0012 range1988422 1 886 926 annotation1988420 1 BBa_B0010 range1988420 1 798 877 annotation1988419 1 BBa_K143037 range1988419 1 1 789 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K143066_sequence 1 atggctagttttcaatcatttgggataccaggacagctggaagtcatcaaaaaagcacttgatcacgtgcgagtcggtgtggtaattacagatcccgcacttgaagataatcctattgtctacgtaaatcaaggctttgttcaaatgaccggctacgagaccgaggaaattttaggaaagaactgtcgcttcttacaggggaaacacacagatccggcagaagtggacaacatcagaaccgctttacaaaataaagaaccggtcaccgttcagatccaaaactacaaaaaagacggaacgatgttctggaatgaattaaatattgatccaatggaaatagaggataaaacgtattttgtcggcattcagaatgatatcaccaagcaaaaagaatatgaaaagcttctcgaggattccctcacggaaattactgcactttcaactcctattgtcccgattcgcaatggcatttcggctcttccgctagtcggaaacctgacagaggagcgatttaattccatcgtttgcacattgacgaatatcttatcaacatccaaagatgattatttgatcattgatttatccggattggcccaagtgaacgaacaaacggccgaccaaattttcaagctgagccatttgctgaaattgaccggaactgagttaatcattactggcattaagcctgaattggctatgaaaatgaataaactggatgccaatttttcgtcgctgaaaacatattcaaatgtaaaggatgccgttaaagtgcttccgattatgtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K143037_sequence 1 atggctagttttcaatcatttgggataccaggacagctggaagtcatcaaaaaagcacttgatcacgtgcgagtcggtgtggtaattacagatcccgcacttgaagataatcctattgtctacgtaaatcaaggctttgttcaaatgaccggctacgagaccgaggaaattttaggaaagaactgtcgcttcttacaggggaaacacacagatccggcagaagtggacaacatcagaaccgctttacaaaataaagaaccggtcaccgttcagatccaaaactacaaaaaagacggaacgatgttctggaatgaattaaatattgatccaatggaaatagaggataaaacgtattttgtcggcattcagaatgatatcaccaagcaaaaagaatatgaaaagcttctcgaggattccctcacggaaattactgcactttcaactcctattgtcccgattcgcaatggcatttcggctcttccgctagtcggaaacctgacagaggagcgatttaattccatcgtttgcacattgacgaatatcttatcaacatccaaagatgattatttgatcattgatttatccggattggcccaagtgaacgaacaaacggccgaccaaattttcaagctgagccatttgctgaaattgaccggaactgagttaatcattactggcattaagcctgaattggctatgaaaatgaataaactggatgccaatttttcgtcgctgaaaacatattcaaatgtaaaggatgccgttaaagtgcttccgattatgtaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z