BBa_K143071
type: ComponentDefinition
persistent identity: BBa K143071
display id: BBa_K143071
version: 1
was generated by: iGEM to SBOL conversion
description: AmyE integratable PoPS generator (P43-gsiB) (with CmR)
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:10:24Z
mutable provenance: The amyE 5' integration sequence was PCR cloned from the ''B. subtilis'' integration vector utilising Pfu DNA polymerase and cloned into a BioBrick with the P43-gsiB that was synthesised by GeneArt and Chloramphenicol adenyltransferase and the double terminator that were obtained from the registry.
owned by: james
owned by: myers
mutable description: AmyE 5' Integration sequence(<bbpart>BBa_K143001</bbpart>) coupled to a chloramphenicol resistance generator in closed transcriptional unit (Parts <bbpart>BBa_K143050</bbpart> and <bbpart>BBa_K143064</bbpart>) and the PoPS generator P43-gsiB (<bbpart>BBa_K143050</bbpart>). The amyE 5' integration sequence allows integration into the ''B. subtilis'' genome at the amyE locus if the 3' amyE integration sequence(<bbpart>BBa_K143002</bbpart>) is cloned onto the 3' end of the construct. The chloramphenicol adenyltransferase give resistance to chloramphenicol while the terminator prevents readthrough. The P43-gsiB promoter and RBS for ''B. subtilis'' constitutively generate a PoPS output.
discontinued: false
dominant: false
experience: experience/None
group u list: _199_
m user id: 0
owner id: 3475
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The amyE 5' integration sequence was PCR cloned from the ''B. subtilis'' integration vector pDR111 utilising Pfu DNA polymerase. The sequence of P43-gsiB was obtained from papers. Chloramphenicol adenyltransferase and the double terminator were obtained from the registry.
star: false
creator: Chris Hirst
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_K143001
component: BBa_B0010
component: BBa_J31005
component: BBa_K143020
component: BBa_K143013
sequence annotation: BBa_K143001
sequence annotation: BBa_K143013
sequence annotation: BBa_K143020
sequence annotation: BBa_J31005
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K143071_sequence/1

BBa_B0012
type: Component
persistent identity: component1991840
display id: component1991840
version: 1
top level: BBa_K143071
definition: BBa_B0012
access: public

BBa_K143001
type: Component
persistent identity: component1991828
display id: component1991828
version: 1
top level: BBa_K143071
definition: amyE 5 IS
access: public

BBa_B0010
type: Component
persistent identity: component1991838
display id: component1991838
version: 1
top level: BBa_K143071
definition: BBa_B0010
access: public

BBa_J31005
type: Component
persistent identity: component1991837
display id: component1991837
version: 1
top level: BBa_K143071
definition: CmR
access: public

BBa_K143020
type: Component
persistent identity: component1991835
display id: component1991835
version: 1
top level: BBa_K143071
definition: RBS-GsiB
access: public

BBa_K143013
type: Component
persistent identity: component1991833
display id: component1991833
version: 1
top level: BBa_K143071
definition: P43
access: public

BBa_K143020
type: Component
persistent identity: component1991850
display id: component1991850
version: 1
top level: BBa_K143071
definition: RBS-GsiB
access: public

BBa_K143013
type: Component
persistent identity: component1991848
display id: component1991848
version: 1
top level: BBa_K143071
definition: P43
access: public

BBa_K143001
type: SequenceAnnotation
persistent identity: annotation1991828
display id: annotation1991828
version: 1
top level: BBa_K143071
location: https://synbiohub.org/public/igem/BBa_K143071/annotation1991828/range1991828/1
component: BBa_K143001

https://synbiohub.org/public/igem/BBa_K143071/annotation1991828/range1991828/1
type: Range
persistent identity: range1991828
display id: range1991828
version: 1
top level: BBa_K143071
direction: direction/reverse
start: 1
end: 522
orientation: inline

BBa_K143013
type: SequenceAnnotation
persistent identity: annotation1991833
display id: annotation1991833
version: 1
top level: BBa_K143071
location: https://synbiohub.org/public/igem/BBa_K143071/annotation1991833/range1991833/1
component: BBa_K143013

https://synbiohub.org/public/igem/BBa_K143071/annotation1991833/range1991833/1
type: Range
persistent identity: range1991833
display id: range1991833
version: 1
top level: BBa_K143071
direction: direction/reverse
start: 531
end: 586
orientation: inline

BBa_K143020
type: SequenceAnnotation
persistent identity: annotation1991835
display id: annotation1991835
version: 1
top level: BBa_K143071
location: https://synbiohub.org/public/igem/BBa_K143071/annotation1991835/range1991835/1
component: BBa_K143020

https://synbiohub.org/public/igem/BBa_K143071/annotation1991835/range1991835/1
type: Range
persistent identity: range1991835
display id: range1991835
version: 1
top level: BBa_K143071
direction: direction/reverse
start: 595
end: 605
orientation: inline

BBa_J31005
type: SequenceAnnotation
persistent identity: annotation1991837
display id: annotation1991837
version: 1
top level: BBa_K143071
location: https://synbiohub.org/public/igem/BBa_K143071/annotation1991837/range1991837/1
component: BBa_J31005

https://synbiohub.org/public/igem/BBa_K143071/annotation1991837/range1991837/1
type: Range
persistent identity: range1991837
display id: range1991837
version: 1
top level: BBa_K143071
direction: direction/reverse
start: 612
end: 1271
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1991838
display id: annotation1991838
version: 1
top level: BBa_K143071
location: https://synbiohub.org/public/igem/BBa_K143071/annotation1991838/range1991838/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K143071/annotation1991838/range1991838/1
type: Range
persistent identity: range1991838
display id: range1991838
version: 1
top level: BBa_K143071
direction: direction/forward
start: 1280
end: 1359
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1991840
display id: annotation1991840
version: 1
top level: BBa_K143071
location: https://synbiohub.org/public/igem/BBa_K143071/annotation1991840/range1991840/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K143071/annotation1991840/range1991840/1
type: Range
persistent identity: range1991840
display id: range1991840
version: 1
top level: BBa_K143071
direction: direction/forward
start: 1368
end: 1408
orientation: inline

BBa_K143013
type: SequenceAnnotation
persistent identity: annotation1991848
display id: annotation1991848
version: 1
top level: BBa_K143071
location: https://synbiohub.org/public/igem/BBa_K143071/annotation1991848/range1991848/1
component: BBa_K143013

https://synbiohub.org/public/igem/BBa_K143071/annotation1991848/range1991848/1
type: Range
persistent identity: range1991848
display id: range1991848
version: 1
top level: BBa_K143071
direction: direction/reverse
start: 1417
end: 1472
orientation: inline

BBa_K143020
type: SequenceAnnotation
persistent identity: annotation1991850
display id: annotation1991850
version: 1
top level: BBa_K143071
location: https://synbiohub.org/public/igem/BBa_K143071/annotation1991850/range1991850/1
component: BBa_K143020

https://synbiohub.org/public/igem/BBa_K143071/annotation1991850/range1991850/1
type: Range
persistent identity: range1991850
display id: range1991850
version: 1
top level: BBa_K143071
direction: direction/reverse
start: 1481
end: 1491
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

amyE 5 IS
type: ComponentDefinition
persistent identity: BBa K143001
display id: BBa_K143001
version: 1
was generated by: iGEM to SBOL conversion
description: 5??? Integration Sequence for the amyE locus of B. subtilis
created: 2008-08-26T11:00:00Z
modified: 2015-05-08T01:10:23Z
mutable provenance: The 5??? integration sequence was taken from the shuttle vector pDR111 which has been used in many studies on ''B.subtilis'', in particular in the studies of transcriptional control#1 #2 #3 <biblio> #1 pmid=14597697 #2 pmid=15937167 #3 pmid=12169614 </biblio>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The 5' integration sequence can be added to the front of a Biobrick construct and the 3' integration sequence specific for this locus (Part BBa_K143002) to the rear of the Biobrick construct to allow integration of the Biobrick construct into the chromosome of the gram positive bacterium B.subtilis. The AmyE locus was the first locus used for integration into ''B.subtilis'' by Shimotsu and Henner#1 and is still commonly used in vectors such as pDR111#2, pDL#3 and their derivatives. Integration at the AmyE locus removes the ability of ''B.subtilis'' to break down starch, which can be assayed with iodine as described by Cutting and Vander-horn#4. The 5' and 3' integration sequences for the AmyE locus were used to integrate the Imperial 2008 iGEM project primary construct into the ''B.sutbilis'' chromosome. <biblio> #1 pmid=3019840 #2 pmid=14597697 #3 ''Bacillus'' Genetic Stock Center [www.bgsc.org] #4 Cutting, S M.; Vander-Horn, P B. Genetic analysis. In: Harwood C R, Cutting S M. , editors. Molecular biological methods for Bacillus. Chichester, England: John Wiley & Sons, Ltd.; 1990. pp. 27???74. </biblio>
discontinued: false
dominant: false
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 3475
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: The AmyE integration sequence was taken from the vector after comparison by BLAST to the ''B.subtilis'' chromosome to identify the homologous sequences. The sequence present in both the host chromosome and the plasmid at the 5' end of the gene is the 5' sequence required for integration.
star: true
creator: Chris Hirst
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: 5' AmyE homologous sequence
sequence: https://synbiohub.org/public/igem/BBa_K143001_sequence/1

5' AmyE homologous sequence
type: SequenceAnnotation
persistent identity: annotation1974145
display id: annotation1974145
version: 1
top level: amyE 5 IS
location: https://synbiohub.org/public/igem/BBa_K143001/annotation1974145/range1974145/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K143001/annotation1974145/range1974145/1
type: Range
persistent identity: range1974145
display id: range1974145
version: 1
top level: amyE 5 IS
direction: direction/forward
start: 1
end: 522
orientation: inline

P43
type: ComponentDefinition
persistent identity: BBa K143013
display id: BBa_K143013
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter 43 a constitutive promoter for B. subtilis
created: 2008-09-10T11:00:00Z
modified: 2015-05-08T01:10:23Z
mutable provenance: The part was designed using the sequence from the ''B.subtilis'' genome and from previously published papers 2. This sequence was then synthesised by Geneart.
owned by: james
owned by: myers
mutable description: Promoter 43 is a constitutive promoter that constitutively expresses the P43 protein in ''B.subtilis''. This promoter has been shown to be recognized and active during the exponential and lag phases of growth. It has been hypothesized that the ability to recognize the promoter in exponential and lag phase of growth is due to the recognition of the promoter by both sigma factor 55 (the major sigma factor) and sigma factor 37 (the lag phase sigma factor) 1. The P43 promoter has been previously used for constitutive expression of exogenous genes within ''B.subtilis'' vectors 2. The context with which we used the promoter P43 is as a '''Polymerase Per Second''' (PoPS) generator.
discontinued: false
dominant: false
experience: experience/None
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The biobrick part was designed to include the binding sites for both the sigma factor A and B. In addition the biobrick standard was applied to the promoter P43 sequence.
star: false
creator: James Chappell
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: Sigma B -35
sequence annotation: Sigma A -35
sequence annotation: Sigma B -10
sequence annotation: Sigma A -10
sequence: https://synbiohub.org/public/igem/BBa_K143013_sequence/1

Sigma B -35
type: SequenceAnnotation
persistent identity: annotation1975706
display id: annotation1975706
version: 1
top level: P43
location: https://synbiohub.org/public/igem/BBa_K143013/annotation1975706/range1975706/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K143013/annotation1975706/range1975706/1
type: Range
persistent identity: range1975706
display id: range1975706
version: 1
top level: P43
direction: direction/forward
start: 14
end: 22
orientation: inline

Sigma A -35
type: SequenceAnnotation
persistent identity: annotation1975708
display id: annotation1975708
version: 1
top level: P43
location: https://synbiohub.org/public/igem/BBa_K143013/annotation1975708/range1975708/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K143013/annotation1975708/range1975708/1
type: Range
persistent identity: range1975708
display id: range1975708
version: 1
top level: P43
direction: direction/forward
start: 24
end: 29
orientation: inline

Sigma B -10
type: SequenceAnnotation
persistent identity: annotation1975707
display id: annotation1975707
version: 1
top level: P43
location: https://synbiohub.org/public/igem/BBa_K143013/annotation1975707/range1975707/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K143013/annotation1975707/range1975707/1
type: Range
persistent identity: range1975707
display id: range1975707
version: 1
top level: P43
direction: direction/forward
start: 38
end: 47
orientation: inline

Sigma A -10
type: SequenceAnnotation
persistent identity: annotation1975709
display id: annotation1975709
version: 1
top level: P43
location: https://synbiohub.org/public/igem/BBa_K143013/annotation1975709/range1975709/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K143013/annotation1975709/range1975709/1
type: Range
persistent identity: range1975709
display id: range1975709
version: 1
top level: P43
direction: direction/forward
start: 47
end: 52
orientation: inline

RBS-GsiB
type: ComponentDefinition
persistent identity: BBa K143020
display id: BBa_K143020
version: 1
was generated by: iGEM to SBOL conversion
description: GsiB ribosome binding site (RBS) for B. subtilis
created: 2008-09-14T11:00:00Z
modified: 2015-05-08T01:10:23Z
mutable provenance: The sequence was taken from a previous research paper [1] and was constructed by Geneart
owned by: james
owned by: myers
mutable description: GsiB is an endogenous ribosome binding site from ''B.subtilis''. The sequence of the gsiB ribosome binding site is '''AAAGGAGG''' which is complementary to the sequence '''UUUCCUCC''' from the 3' region of the 16s rRNA from ''B.subtilis''. GsiB is an endogenous ribosome binding site (RBS) from ''B.subtilis''. The sequence of the gsiB ribosome binding site is '''AAAGGAGG''' which is complementary to the sequence '''UUUCCUCC''' from the 3' region of the 16s rRNA from ''B.subtilis''. Previous research showed that the predicted binding energy of the 16s rRNA to the RBS is -9.3kcal.
discontinued: false
dominant: false
experience: experience/None
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: In order to ensure that the RBS is functional the actual ribosome binding site was maintained and the distance between the RBS and the start codon maintained. In order to conform to the biobrick standard the sequence flanking the RBS had to be changed but the distance between the promoter and RBS, and start codon and RBS was maintained.
star: false
creator: James Chappell
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K143020_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1975872
display id: annotation1975872
version: 1
top level: RBS-GsiB
location: https://synbiohub.org/public/igem/BBa_K143020/annotation1975872/range1975872/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K143020/annotation1975872/range1975872/1
type: Range
persistent identity: range1975872
display id: range1975872
version: 1
top level: RBS-GsiB
direction: direction/forward
start: 2
end: 8
orientation: inline

CmR
type: ComponentDefinition
persistent identity: BBa J31005
display id: BBa_J31005
version: 1
was generated by: iGEM to SBOL conversion
description: chloramphenicol acetyltransferase (forwards, CmF) [cf. BBa_J31004]
created: 2006-07-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
mutable provenance: pSB1AC3
owned by: james
owned by: myers
mutable description: When a promoter and an RBS are in front of the gene, the cell will express Chloramphenicol resistance. Because it contains full biobrick ends, this part can be used to easily add chloramphenicol resistance to any part without changing plasmid vectors.
discontinued: false
dominant: true
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 918
owning group id: 61
sample status: In stock
status: status/Available
bookmark: true
mutable notes: This part is cloned into pSB1A2.
star: true
creator: Erin Zwack, Sabriya Rosemond
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CmR gene
sequence: https://synbiohub.org/public/igem/BBa_J31005_sequence/1

CmR gene
type: SequenceAnnotation
persistent identity: annotation1884999
display id: annotation1884999
version: 1
top level: CmR
location: https://synbiohub.org/public/igem/BBa_J31005/annotation1884999/range1884999/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J31005/annotation1884999/range1884999/1
type: Range
persistent identity: range1884999
display id: range1884999
version: 1
top level: CmR
direction: direction/forward
start: 1
end: 660
orientation: inline

https://synbiohub.org/public/igem/BBa_K143071_sequence/1
type: Sequence
persistent identity: BBa K143071 sequence
display id: BBa_K143071_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtttgcaaaacgattcaaaacctctttactgccgttattcgctggatttttattgctgtttcatttggttctggcaggaccggcggctgcgagtgctgaaacggcgaacaaatcgaatgagcttacagcaccgtcgatcaaaagcggaaccattcttcatgcatggaattggtcgttcaatacgttaaaacacaatatgaaggatattcatgatgcaggatatacagccattcagacatctccgattaaccaagtaaaggaagggaatcaaggagataaaagcatgtcgaactggtactggctgtatcagccgacatcgtatcaaattggcaaccgttacttaggtactgaacaagaatttaaagaaatgtgtgcagccgctgaagaatatggcataaaggtcattgttgacgcggtcatcaatcataccaccagtgattatgccgcgatttccaatgaggttaagagtattccaaactggacacatggaaacacacaaattaaaaactggtctgatcgatactagagattttacatttttagaaatgggcgtgaaaaaaagcgcgcgattatgtaaaatataatactagagtaaaggaggaatactagatggagaaaaaaatcactggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctcatccggaatttcgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcaccatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgattcaggttcatcatgccgtttgtgatggcttccatgtcggcagaatgcttaatgaattacaacagtactgcgatgagtggcagggcggggcgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagattttacatttttagaaatgggcgtgaaaaaaagcgcgcgattatgtaaaatataatactagagtaaaggaggaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143001_sequence/1
type: Sequence
persistent identity: BBa K143001 sequence
display id: BBa_K143001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtttgcaaaacgattcaaaacctctttactgccgttattcgctggatttttattgctgtttcatttggttctggcaggaccggcggctgcgagtgctgaaacggcgaacaaatcgaatgagcttacagcaccgtcgatcaaaagcggaaccattcttcatgcatggaattggtcgttcaatacgttaaaacacaatatgaaggatattcatgatgcaggatatacagccattcagacatctccgattaaccaagtaaaggaagggaatcaaggagataaaagcatgtcgaactggtactggctgtatcagccgacatcgtatcaaattggcaaccgttacttaggtactgaacaagaatttaaagaaatgtgtgcagccgctgaagaatatggcataaaggtcattgttgacgcggtcatcaatcataccaccagtgattatgccgcgatttccaatgaggttaagagtattccaaactggacacatggaaacacacaaattaaaaactggtctgatcga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143013_sequence/1
type: Sequence
persistent identity: BBa K143013 sequence
display id: BBa_K143013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attttacatttttagaaatgggcgtgaaaaaaagcgcgcgattatgtaaaatataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143020_sequence/1
type: Sequence
persistent identity: BBa K143020 sequence
display id: BBa_K143020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taaaggaggaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J31005_sequence/1
type: Sequence
persistent identity: BBa J31005 sequence
display id: BBa_J31005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggagaaaaaaatcactggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctcatccggaatttcgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcaccatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgattcaggttcatcatgccgtttgtgatggcttccatgtcggcagaatgcttaatgaattacaacagtactgcgatgagtggcagggcggggcgtaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z