LOCUS       BBa_K143073             1573 bp    DNA     linear       22-Dec-2024
DEFINITION  AmyE integratable PoPS generator (Pveg-gsiB) (with CmR)
ACCESSION   BBa_K143073
VERSION     BBa_K143073.1  
FEATURES             Location/Qualifiers
     misc_feature    1..522
                     /label=5' AmyE homologous sequence
     misc_feature    1..522
                     /label=BBa_K143001
     promoter        593..598
                     /label=Sigma A-35
     promoter        616..621
                     /label=Sigma A -10
     promoter        531..627
                     /label=BBa_K143012
     RBS             637..643
                     /label=rbs
     RBS             636..646
                     /label=BBa_K143020
     misc_feature    653..1312
                     /label=CmR gene
     CDS             653..1312
                     /label=BBa_J31005
     stem_loop       1332..1375
                     /label=stem_loop
     misc_feature    1321..1400
                     /label=BBa_B0010
     terminator      1321..1400
                     /label=BBa_B0010
     stem_loop       1416..1435
                     /label=T7 TE
     misc_feature    1442..1442
                     /label=stop
     polyA_site      1436..1449
                     /label=polya
     misc_feature    1409..1449
                     /label=BBa_B0012
     terminator      1409..1449
                     /label=BBa_B0012
     promoter        1520..1525
                     /label=Sigma A-35
     promoter        1543..1548
                     /label=Sigma A -10
     promoter        1458..1554
                     /label=BBa_K143012
     RBS             1564..1570
                     /label=rbs
     RBS             1563..1573
                     /label=BBa_K143020
ORIGIN
        1 atgtttgcaa aacgattcaa aacctcttta ctgccgttat tcgctggatt tttattgctg
       61 tttcatttgg ttctggcagg accggcggct gcgagtgctg aaacggcgaa caaatcgaat
      121 gagcttacag caccgtcgat caaaagcgga accattcttc atgcatggaa ttggtcgttc
      181 aatacgttaa aacacaatat gaaggatatt catgatgcag gatatacagc cattcagaca
      241 tctccgatta accaagtaaa ggaagggaat caaggagata aaagcatgtc gaactggtac
      301 tggctgtatc agccgacatc gtatcaaatt ggcaaccgtt acttaggtac tgaacaagaa
      361 tttaaagaaa tgtgtgcagc cgctgaagaa tatggcataa aggtcattgt tgacgcggtc
      421 atcaatcata ccaccagtga ttatgccgcg atttccaatg aggttaagag tattccaaac
      481 tggacacatg gaaacacaca aattaaaaac tggtctgatc gatactagag aattttgtca
      541 aaataatttt attgacaacg tcttattaac gttgatataa tttaaatttt atttgacaaa
      601 aatgggctcg tgttgtacaa taaatgttac tagagtaaag gaggaatact agatggagaa
      661 aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga
      721 ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc
      781 ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct
      841 tgcccgcctg atgaatgctc atccggaatt tcgtatggca atgaaagacg gtgagctggt
      901 gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc
      961 atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga
     1021 tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt
     1081 tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat
     1141 ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt
     1201 gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag
     1261 aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt aatactagag
     1321 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     1381 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     1441 gcgtttatat actagagaat tttgtcaaaa taattttatt gacaacgtct tattaacgtt
     1501 gatataattt aaattttatt tgacaaaaat gggctcgtgt tgtacaataa atgttactag
     1561 agtaaaggag gaa
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