LOCUS       BBa_K143076             1853 bp    DNA     linear       22-Dec-2024
DEFINITION  B. subtilis AmyE locus RFP output promoter and RBS tester
ACCESSION   BBa_K143076
VERSION     BBa_K143076.1  
FEATURES             Location/Qualifiers
     CDS             1..675
                     /label=mrfp1
     misc_feature    682..706
                     /label=Help:Barcodes
     CDS             1..681
                     /label=BBa_E1010
     stem_loop       726..769
                     /label=stem_loop
     misc_feature    715..794
                     /label=BBa_B0010
     terminator      715..794
                     /label=BBa_B0010
     stem_loop       810..829
                     /label=T7 TE
     misc_feature    836..836
                     /label=stop
     polyA_site      830..843
                     /label=polya
     misc_feature    803..843
                     /label=BBa_B0012
     terminator      803..843
                     /label=BBa_B0012
     misc_feature    1..843
                     /label=BBa_J04650
     misc_feature    852..1856
                     /label=3' AmyE homologous sequence
     misc_feature    852..1853
                     /label=BBa_K143002
ORIGIN
        1 atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt
       61 tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt
      121 acccagaccg ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc
      181 ctgtccccgc agttccagta cggttccaaa gcttacgtta aacacccggc tgacatcccg
      241 gactacctga aactgtcctt cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa
      301 gacggtggtg ttgttaccgt tacccaggac tcctccctgc aagacggtga gttcatctac
      361 aaagttaaac tgcgtggtac caacttcccg tccgacggtc cggttatgca gaaaaaaacc
      421 atgggttggg aagcttccac cgaacgtatg tacccggaag acggtgctct gaaaggtgaa
      481 atcaaaatgc gtctgaaact gaaagacggt ggtcactacg acgctgaagt taaaaccacc
      541 tacatggcta aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat caaactggac
      601 atcacctccc acaacgaaga ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt
      661 cactccaccg gtgcttaata acgctgatag tgctagtgta gatcgctact agagccaggc
      721 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      781 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
      841 atatactaga gatccgttta ggctgggcgg tgatagcttc tcgttcaggc agtacgcctc
      901 ttttcttttc cagacctgag ggaggcggaa atggtgtgag gttcccgggg aaaagccaaa
      961 taggcgatcg cgggagtgct ttatttgaag atcaggctat cactgcggtc aatagatttc
     1021 acaatgtgat ggctggacag cctgaggaac tctcgaaccc gaatggaaac aaccagatat
     1081 ttatgaatca gcgcggctca catggcgttg tgctggcaaa tgcaggttca tcctctgtct
     1141 ctatcaatac ggcaacaaaa ttgcctgatg gcaggtatga caataaagct ggagcgggtt
     1201 catttcaagt gaacgatggt aaactgacag gcacgatcaa tgccaggtct gtagctgtgc
     1261 tttatcctga tgatattgca aaagcgcctc atgttttcct tgagaattac aaaacaggtg
     1321 taacacattc tttcaatgat caactgacga ttaccttgcg tgcagatgcg aatacaacaa
     1381 aagccgttta tcaaatcaat aatggaccag agacggcgtt taaggatgga gatcaattca
     1441 caatcggaaa aggagatcca tttggcaaaa catacaccat catgttaaaa ggaacgaaca
     1501 gtgatggtgt aacgaggacc gagaaataca gttttgttaa aagagatcca gcgtcggcca
     1561 aaaccatcgg ctatcaaaat ccgaatcatt ggagccaggt aaatgcttat atctataaac
     1621 atgatgggag ccgagtaatt gaattgaccg gatcttggcc tggaaaacca atgactaaaa
     1681 atgcagacgg aatttacacg ctgacgctgc ctgcggacac ggatacaacc aacgcaaaag
     1741 tgatttttaa taatggcagc gcccaagtgc ccggtcagaa tcagcctggc tttgattacg
     1801 tgctaaatgg tttatataat gactcgggct taagcggttc tcttccccat tga
//