LOCUS BBa_K143077 3364 bp DNA linear 22-Nov-2024 DEFINITION P43-gsiB GFP expression construct ACCESSION BBa_K143077 VERSION BBa_K143077.1 FEATURES Location/Qualifiers misc_feature 1..522 /label=5' AmyE homologous sequence misc_feature 1..522 /label=BBa_K143001 promoter 544..552 /label=Sigma B -35 promoter 568..577 /label=Sigma B -10 promoter 554..559 /label=Sigma A -35 promoter 577..582 /label=Sigma A -10 promoter 531..586 /label=BBa_K143013 RBS 596..602 /label=rbs RBS 595..605 /label=BBa_K143020 misc_feature 612..1271 /label=CmR gene CDS 612..1271 /label=BBa_J31005 stem_loop 1291..1334 /label=stem_loop misc_feature 1280..1359 /label=BBa_B0010 terminator 1280..1359 /label=BBa_B0010 stem_loop 1375..1394 /label=T7 TE misc_feature 1401..1401 /label=stop polyA_site 1395..1408 /label=polya misc_feature 1368..1408 /label=BBa_B0012 terminator 1368..1408 /label=BBa_B0012 promoter 1430..1438 /label=Sigma B -35 promoter 1454..1463 /label=Sigma B -10 promoter 1440..1445 /label=Sigma A -35 promoter 1463..1468 /label=Sigma A -10 promoter 1417..1472 /label=BBa_K143013 RBS 1482..1488 /label=rbs RBS 1481..1491 /label=BBa_K143020 misc_feature 1..1491 /label=BBa_K143071 CDS 1498..2217 /label=GFP protein CDS 1498..2217 /label=BBa_E0040 stem_loop 2237..2280 /label=stem_loop misc_feature 2226..2305 /label=BBa_B0010 terminator 2226..2305 /label=BBa_B0010 stem_loop 2321..2340 /label=T7 TE misc_feature 2347..2347 /label=stop polyA_site 2341..2354 /label=polya misc_feature 2314..2354 /label=BBa_B0012 terminator 2314..2354 /label=BBa_B0012 misc_feature 1498..2354 /label=BBa_I13401 misc_feature 2363..3367 /label=3' AmyE homologous sequence misc_feature 2363..3364 /label=BBa_K143002 misc_feature 1498..3364 /label=BBa_K143075 ORIGIN 1 atgtttgcaa aacgattcaa aacctcttta ctgccgttat tcgctggatt tttattgctg 61 tttcatttgg ttctggcagg accggcggct gcgagtgctg aaacggcgaa caaatcgaat 121 gagcttacag caccgtcgat caaaagcgga accattcttc atgcatggaa ttggtcgttc 181 aatacgttaa aacacaatat gaaggatatt catgatgcag gatatacagc cattcagaca 241 tctccgatta accaagtaaa ggaagggaat caaggagata aaagcatgtc gaactggtac 301 tggctgtatc agccgacatc gtatcaaatt ggcaaccgtt acttaggtac tgaacaagaa 361 tttaaagaaa tgtgtgcagc cgctgaagaa tatggcataa aggtcattgt tgacgcggtc 421 atcaatcata ccaccagtga ttatgccgcg atttccaatg aggttaagag tattccaaac 481 tggacacatg gaaacacaca aattaaaaac tggtctgatc gatactagag attttacatt 541 tttagaaatg ggcgtgaaaa aaagcgcgcg attatgtaaa atataatact agagtaaagg 601 aggaatacta gatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc 661 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg 721 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc 781 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattt cgtatggcaa 841 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg 901 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc 961 tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag 1021 ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg 1081 atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt 1141 atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg 1201 atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg 1261 gcggggcgta atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 1321 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca 1381 ccttcgggtg ggcctttctg cgtttatata ctagagattt tacattttta gaaatgggcg 1441 tgaaaaaaag cgcgcgatta tgtaaaatat aatactagag taaaggagga atactagatg 1501 cgtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat 1561 gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa 1621 cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc 1681 actactttcg gttatggtgt tcaatgcttt gcgagatacc cagatcatat gaaacagcat 1741 gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa 1801 gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat 1861 agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg 1921 gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc 1981 aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat 2041 tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg 2101 tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt 2161 gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa ataataatac 2221 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 2281 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc 2341 tttctgcgtt tatatactag agatccgttt aggctgggcg gtgatagctt ctcgttcagg 2401 cagtacgcct cttttctttt ccagacctga gggaggcgga aatggtgtga ggttcccggg 2461 gaaaagccaa ataggcgatc gcgggagtgc tttatttgaa gatcaggcta tcactgcggt 2521 caatagattt cacaatgtga tggctggaca gcctgaggaa ctctcgaacc cgaatggaaa 2581 caaccagata tttatgaatc agcgcggctc acatggcgtt gtgctggcaa atgcaggttc 2641 atcctctgtc tctatcaata cggcaacaaa attgcctgat ggcaggtatg acaataaagc 2701 tggagcgggt tcatttcaag tgaacgatgg taaactgaca ggcacgatca atgccaggtc 2761 tgtagctgtg ctttatcctg atgatattgc aaaagcgcct catgttttcc ttgagaatta 2821 caaaacaggt gtaacacatt ctttcaatga tcaactgacg attaccttgc gtgcagatgc 2881 gaatacaaca aaagccgttt atcaaatcaa taatggacca gagacggcgt ttaaggatgg 2941 agatcaattc acaatcggaa aaggagatcc atttggcaaa acatacacca tcatgttaaa 3001 aggaacgaac agtgatggtg taacgaggac cgagaaatac agttttgtta aaagagatcc 3061 agcgtcggcc aaaaccatcg gctatcaaaa tccgaatcat tggagccagg taaatgctta 3121 tatctataaa catgatggga gccgagtaat tgaattgacc ggatcttggc ctggaaaacc 3181 aatgactaaa aatgcagacg gaatttacac gctgacgctg cctgcggaca cggatacaac 3241 caacgcaaaa gtgattttta ataatggcag cgcccaagtg cccggtcaga atcagcctgg 3301 ctttgattac gtgctaaatg gtttatataa tgactcgggc ttaagcggtt ctcttcccca 3361 ttga //