LOCUS BBa_K143079 3448 bp DNA linear 22-Nov-2024 DEFINITION Pveg-spoVG GFP expression construct ACCESSION BBa_K143079 VERSION BBa_K143079.1 FEATURES Location/Qualifiers misc_feature 1..522 /label=5' AmyE homologous sequence misc_feature 1..522 /label=BBa_K143001 promoter 593..598 /label=Sigma A-35 promoter 616..621 /label=Sigma A -10 promoter 531..627 /label=BBa_K143012 RBS 636..647 /label=rbs RBS 636..647 /label=BBa_K143021 misc_feature 654..1313 /label=CmR gene CDS 654..1313 /label=BBa_J31005 stem_loop 1333..1376 /label=stem_loop misc_feature 1322..1401 /label=BBa_B0010 terminator 1322..1401 /label=BBa_B0010 stem_loop 1417..1436 /label=T7 TE misc_feature 1443..1443 /label=stop polyA_site 1437..1450 /label=polya misc_feature 1410..1450 /label=BBa_B0012 terminator 1410..1450 /label=BBa_B0012 promoter 1521..1526 /label=Sigma A-35 promoter 1544..1549 /label=Sigma A -10 promoter 1459..1555 /label=BBa_K143012 RBS 1564..1575 /label=rbs RBS 1564..1575 /label=BBa_K143021 misc_feature 1..1575 /label=BBa_K143074 CDS 1582..2301 /label=GFP protein CDS 1582..2301 /label=BBa_E0040 stem_loop 2321..2364 /label=stem_loop misc_feature 2310..2389 /label=BBa_B0010 terminator 2310..2389 /label=BBa_B0010 stem_loop 2405..2424 /label=T7 TE misc_feature 2431..2431 /label=stop polyA_site 2425..2438 /label=polya misc_feature 2398..2438 /label=BBa_B0012 terminator 2398..2438 /label=BBa_B0012 misc_feature 1582..2438 /label=BBa_I13401 misc_feature 2447..3451 /label=3' AmyE homologous sequence misc_feature 2447..3448 /label=BBa_K143002 misc_feature 1582..3448 /label=BBa_K143075 ORIGIN 1 atgtttgcaa aacgattcaa aacctcttta ctgccgttat tcgctggatt tttattgctg 61 tttcatttgg ttctggcagg accggcggct gcgagtgctg aaacggcgaa caaatcgaat 121 gagcttacag caccgtcgat caaaagcgga accattcttc atgcatggaa ttggtcgttc 181 aatacgttaa aacacaatat gaaggatatt catgatgcag gatatacagc cattcagaca 241 tctccgatta accaagtaaa ggaagggaat caaggagata aaagcatgtc gaactggtac 301 tggctgtatc agccgacatc gtatcaaatt ggcaaccgtt acttaggtac tgaacaagaa 361 tttaaagaaa tgtgtgcagc cgctgaagaa tatggcataa aggtcattgt tgacgcggtc 421 atcaatcata ccaccagtga ttatgccgcg atttccaatg aggttaagag tattccaaac 481 tggacacatg gaaacacaca aattaaaaac tggtctgatc gatactagag aattttgtca 541 aaataatttt attgacaacg tcttattaac gttgatataa tttaaatttt atttgacaaa 601 aatgggctcg tgttgtacaa taaatgttac tagagaaagg tggtgaatac tagatggaga 661 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg 721 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg 781 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc 841 ttgcccgcct gatgaatgct catccggaat ttcgtatggc aatgaaagac ggtgagctgg 901 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt 961 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag 1021 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt 1081 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata 1141 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg 1201 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca 1261 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taatactaga 1321 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 1381 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc 1441 tgcgtttata tactagagaa ttttgtcaaa ataattttat tgacaacgtc ttattaacgt 1501 tgatataatt taaattttat ttgacaaaaa tgggctcgtg ttgtacaata aatgttacta 1561 gagaaaggtg gtgaatacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca 1621 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt 1681 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta 1741 cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga 1801 tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta 1861 caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag 1921 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat 1981 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg 2041 gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat 2101 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc 2161 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa 2221 aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg 2281 gatgaactat acaaataata atactagagc caggcatcaa ataaaacgaa aggctcagtc 2341 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 2401 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagatcc gtttaggctg 2461 ggcggtgata gcttctcgtt caggcagtac gcctcttttc ttttccagac ctgagggagg 2521 cggaaatggt gtgaggttcc cggggaaaag ccaaataggc gatcgcggga gtgctttatt 2581 tgaagatcag gctatcactg cggtcaatag atttcacaat gtgatggctg gacagcctga 2641 ggaactctcg aacccgaatg gaaacaacca gatatttatg aatcagcgcg gctcacatgg 2701 cgttgtgctg gcaaatgcag gttcatcctc tgtctctatc aatacggcaa caaaattgcc 2761 tgatggcagg tatgacaata aagctggagc gggttcattt caagtgaacg atggtaaact 2821 gacaggcacg atcaatgcca ggtctgtagc tgtgctttat cctgatgata ttgcaaaagc 2881 gcctcatgtt ttccttgaga attacaaaac aggtgtaaca cattctttca atgatcaact 2941 gacgattacc ttgcgtgcag atgcgaatac aacaaaagcc gtttatcaaa tcaataatgg 3001 accagagacg gcgtttaagg atggagatca attcacaatc ggaaaaggag atccatttgg 3061 caaaacatac accatcatgt taaaaggaac gaacagtgat ggtgtaacga ggaccgagaa 3121 atacagtttt gttaaaagag atccagcgtc ggccaaaacc atcggctatc aaaatccgaa 3181 tcattggagc caggtaaatg cttatatcta taaacatgat gggagccgag taattgaatt 3241 gaccggatct tggcctggaa aaccaatgac taaaaatgca gacggaattt acacgctgac 3301 gctgcctgcg gacacggata caaccaacgc aaaagtgatt tttaataatg gcagcgccca 3361 agtgcccggt cagaatcagc ctggctttga ttacgtgcta aatggtttat ataatgactc 3421 gggcttaagc ggttctcttc cccattga //