##gff-version 3
##sequence-region BBa_K143079 1 3448
BBa_K143079	.	engineered_region	1	1575	.	+	0	ID=BBa_K143074;Name=BBa_K143074
BBa_K143079	.	ribosome_entry_site	636	647	.	+	0	ID=BBa_K143021;Name=BBa_K143021;Parent=BBa_K143074
BBa_K143079	.	ribosome_entry_site	636	647	.	+	0	ID=annotation1975997;Name=rbs;Parent=BBa_K143021
BBa_K143079	.	promoter	531	627	.	+	0	ID=BBa_K143012;Name=BBa_K143012;Parent=BBa_K143074
BBa_K143079	.	promoter	593	598	.	+	0	ID=annotation1975704;Name=Sigma A-35;Parent=BBa_K143012
BBa_K143079	.	promoter	616	621	.	+	0	ID=annotation1975705;Name=Sigma A -10;Parent=BBa_K143012
BBa_K143079	.	CDS	654	1313	.	+	0	ID=BBa_J31005;Name=BBa_J31005;Parent=BBa_K143074
BBa_K143079	.	sequence_feature	654	1313	.	+	0	ID=annotation1884999;Name=CmR gene;Parent=BBa_J31005
BBa_K143079	.	terminator	1410	1450	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K143074
BBa_K143079	.	engineered_region	1410	1450	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K143079	.	stop_codon	1443	1443	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K143079	.	stem_loop	1417	1436	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K143079	.	polyA_site	1437	1450	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K143079	.	promoter	1459	1555	.	+	0	ID=BBa_K143012;Name=BBa_K143012;Parent=BBa_K143074
BBa_K143079	.	promoter	1521	1526	.	+	0	ID=annotation1975704;Name=Sigma A-35;Parent=BBa_K143012
BBa_K143079	.	promoter	1544	1549	.	+	0	ID=annotation1975705;Name=Sigma A -10;Parent=BBa_K143012
BBa_K143079	.	terminator	1322	1401	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K143074
BBa_K143079	.	stem_loop	1333	1376	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K143079	.	engineered_region	1322	1401	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K143079	.	sequence_feature	1	522	.	+	0	ID=BBa_K143001;Name=BBa_K143001;Parent=BBa_K143074
BBa_K143079	.	sequence_feature	1	522	.	+	0	ID=annotation1974145;Name=5' AmyE homologous sequence;Parent=BBa_K143001
BBa_K143079	.	ribosome_entry_site	1564	1575	.	+	0	ID=BBa_K143021;Name=BBa_K143021;Parent=BBa_K143074
BBa_K143079	.	ribosome_entry_site	1564	1575	.	+	0	ID=annotation1975997;Name=rbs;Parent=BBa_K143021
BBa_K143079	.	engineered_region	1582	3448	.	+	0	ID=BBa_K143075;Name=BBa_K143075
BBa_K143079	.	sequence_feature	2447	3448	.	+	0	ID=BBa_K143002;Name=BBa_K143002;Parent=BBa_K143075
BBa_K143079	.	sequence_feature	2447	3451	.	+	0	ID=annotation1974146;Name=3' AmyE homologous sequence;Parent=BBa_K143002
BBa_K143079	.	engineered_region	1582	2438	.	+	0	ID=BBa_I13401;Name=BBa_I13401;Parent=BBa_K143075
BBa_K143079	.	terminator	2398	2438	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13401
BBa_K143079	.	engineered_region	2398	2438	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K143079	.	stop_codon	2431	2431	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K143079	.	stem_loop	2405	2424	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K143079	.	polyA_site	2425	2438	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K143079	.	terminator	2310	2389	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13401
BBa_K143079	.	stem_loop	2321	2364	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K143079	.	engineered_region	2310	2389	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K143079	.	CDS	1582	2301	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_I13401
BBa_K143079	.	CDS	1582	2301	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
>BBa_K143079
atgtttgcaaaacgattcaaaacctctttactgccgttattcgctggatttttattgctgtttcatttggttctggcag
gaccggcggctgcgagtgctgaaacggcgaacaaatcgaatgagcttacagcaccgtcgatcaaaagcggaaccattct
tcatgcatggaattggtcgttcaatacgttaaaacacaatatgaaggatattcatgatgcaggatatacagccattcag
acatctccgattaaccaagtaaaggaagggaatcaaggagataaaagcatgtcgaactggtactggctgtatcagccga
catcgtatcaaattggcaaccgttacttaggtactgaacaagaatttaaagaaatgtgtgcagccgctgaagaatatgg
cataaaggtcattgttgacgcggtcatcaatcataccaccagtgattatgccgcgatttccaatgaggttaagagtatt
ccaaactggacacatggaaacacacaaattaaaaactggtctgatcgatactagagaattttgtcaaaataattttatt
gacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgttacta
gagaaaggtggtgaatactagatggagaaaaaaatcactggatataccaccgttgatatatcccaatggcatcgtaaag
aacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaa
gaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctcatccggaa
tttcgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgttacaccgttttccatgagcaaa
ctgaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttctacacatatattcgcaagatgtggc
gtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctgggtg
agtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcaccatgggcaaatattata
cgcaaggcgacaaggtgctgatgccgctggcgattcaggttcatcatgccgtttgtgatggcttccatgtcggcagaat
gcttaatgaattacaacagtactgcgatgagtggcagggcggggcgtaatactagagccaggcatcaaataaaacgaaa
ggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctca
ccttcgggtgggcctttctgcgtttatatactagagaattttgtcaaaataattttattgacaacgtcttattaacgtt
gatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgttactagagaaaggtggtgaatacta
gatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaa
ttttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaac
tacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaa
acagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaac
tacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaag
aagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaa
gaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaa
aatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatc
ccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactata
caaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg
tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagatc
cgtttaggctgggcggtgatagcttctcgttcaggcagtacgcctcttttcttttccagacctgagggaggcggaaatg
gtgtgaggttcccggggaaaagccaaataggcgatcgcgggagtgctttatttgaagatcaggctatcactgcggtcaa
tagatttcacaatgtgatggctggacagcctgaggaactctcgaacccgaatggaaacaaccagatatttatgaatcag
cgcggctcacatggcgttgtgctggcaaatgcaggttcatcctctgtctctatcaatacggcaacaaaattgcctgatg
gcaggtatgacaataaagctggagcgggttcatttcaagtgaacgatggtaaactgacaggcacgatcaatgccaggtc
tgtagctgtgctttatcctgatgatattgcaaaagcgcctcatgttttccttgagaattacaaaacaggtgtaacacat
tctttcaatgatcaactgacgattaccttgcgtgcagatgcgaatacaacaaaagccgtttatcaaatcaataatggac
cagagacggcgtttaaggatggagatcaattcacaatcggaaaaggagatccatttggcaaaacatacaccatcatgtt
aaaaggaacgaacagtgatggtgtaacgaggaccgagaaatacagttttgttaaaagagatccagcgtcggccaaaacc
atcggctatcaaaatccgaatcattggagccaggtaaatgcttatatctataaacatgatgggagccgagtaattgaat
tgaccggatcttggcctggaaaaccaatgactaaaaatgcagacggaatttacacgctgacgctgcctgcggacacgga
tacaaccaacgcaaaagtgatttttaataatggcagcgcccaagtgcccggtcagaatcagcctggctttgattacgtg
ctaaatggtttatataatgactcgggcttaagcggttctcttccccattga