LOCUS BBa_K143080 3350 bp DNA linear 22-Nov-2024 DEFINITION P43-gsiB RFP expression construct ACCESSION BBa_K143080 VERSION BBa_K143080.1 FEATURES Location/Qualifiers misc_feature 1..522 /label=5' AmyE homologous sequence misc_feature 1..522 /label=BBa_K143001 promoter 544..552 /label=Sigma B -35 promoter 568..577 /label=Sigma B -10 promoter 554..559 /label=Sigma A -35 promoter 577..582 /label=Sigma A -10 promoter 531..586 /label=BBa_K143013 RBS 596..602 /label=rbs RBS 595..605 /label=BBa_K143020 misc_feature 612..1271 /label=CmR gene CDS 612..1271 /label=BBa_J31005 stem_loop 1291..1334 /label=stem_loop misc_feature 1280..1359 /label=BBa_B0010 terminator 1280..1359 /label=BBa_B0010 stem_loop 1375..1394 /label=T7 TE misc_feature 1401..1401 /label=stop polyA_site 1395..1408 /label=polya misc_feature 1368..1408 /label=BBa_B0012 terminator 1368..1408 /label=BBa_B0012 promoter 1430..1438 /label=Sigma B -35 promoter 1454..1463 /label=Sigma B -10 promoter 1440..1445 /label=Sigma A -35 promoter 1463..1468 /label=Sigma A -10 promoter 1417..1472 /label=BBa_K143013 RBS 1482..1488 /label=rbs RBS 1481..1491 /label=BBa_K143020 misc_feature 1..1491 /label=BBa_K143071 CDS 1498..2172 /label=mrfp1 misc_feature 2179..2203 /label=Help:Barcodes CDS 1498..2178 /label=BBa_E1010 stem_loop 2223..2266 /label=stem_loop misc_feature 2212..2291 /label=BBa_B0010 terminator 2212..2291 /label=BBa_B0010 stem_loop 2307..2326 /label=T7 TE misc_feature 2333..2333 /label=stop polyA_site 2327..2340 /label=polya misc_feature 2300..2340 /label=BBa_B0012 terminator 2300..2340 /label=BBa_B0012 misc_feature 1498..2340 /label=BBa_J04650 misc_feature 2349..3353 /label=3' AmyE homologous sequence misc_feature 2349..3350 /label=BBa_K143002 misc_feature 1498..3350 /label=BBa_K143076 ORIGIN 1 atgtttgcaa aacgattcaa aacctcttta ctgccgttat tcgctggatt tttattgctg 61 tttcatttgg ttctggcagg accggcggct gcgagtgctg aaacggcgaa caaatcgaat 121 gagcttacag caccgtcgat caaaagcgga accattcttc atgcatggaa ttggtcgttc 181 aatacgttaa aacacaatat gaaggatatt catgatgcag gatatacagc cattcagaca 241 tctccgatta accaagtaaa ggaagggaat caaggagata aaagcatgtc gaactggtac 301 tggctgtatc agccgacatc gtatcaaatt ggcaaccgtt acttaggtac tgaacaagaa 361 tttaaagaaa tgtgtgcagc cgctgaagaa tatggcataa aggtcattgt tgacgcggtc 421 atcaatcata ccaccagtga ttatgccgcg atttccaatg aggttaagag tattccaaac 481 tggacacatg gaaacacaca aattaaaaac tggtctgatc gatactagag attttacatt 541 tttagaaatg ggcgtgaaaa aaagcgcgcg attatgtaaa atataatact agagtaaagg 601 aggaatacta gatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc 661 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg 721 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc 781 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattt cgtatggcaa 841 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg 901 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc 961 tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag 1021 ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg 1081 atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt 1141 atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg 1201 atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg 1261 gcggggcgta atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 1321 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca 1381 ccttcgggtg ggcctttctg cgtttatata ctagagattt tacattttta gaaatgggcg 1441 tgaaaaaaag cgcgcgatta tgtaaaatat aatactagag taaaggagga atactagatg 1501 gcttcctccg aagacgttat caaagagttc atgcgtttca aagttcgtat ggaaggttcc 1561 gttaacggtc acgagttcga aatcgaaggt gaaggtgaag gtcgtccgta cgaaggtacc 1621 cagaccgcta aactgaaagt taccaaaggt ggtccgctgc cgttcgcttg ggacatcctg 1681 tccccgcagt tccagtacgg ttccaaagct tacgttaaac acccggctga catcccggac 1741 tacctgaaac tgtccttccc ggaaggtttc aaatgggaac gtgttatgaa cttcgaagac 1801 ggtggtgttg ttaccgttac ccaggactcc tccctgcaag acggtgagtt catctacaaa 1861 gttaaactgc gtggtaccaa cttcccgtcc gacggtccgg ttatgcagaa aaaaaccatg 1921 ggttgggaag cttccaccga acgtatgtac ccggaagacg gtgctctgaa aggtgaaatc 1981 aaaatgcgtc tgaaactgaa agacggtggt cactacgacg ctgaagttaa aaccacctac 2041 atggctaaaa aaccggttca gctgccgggt gcttacaaaa ccgacatcaa actggacatc 2101 acctcccaca acgaagacta caccatcgtt gaacagtacg aacgtgctga aggtcgtcac 2161 tccaccggtg cttaataacg ctgatagtgc tagtgtagat cgctactaga gccaggcatc 2221 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 2281 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata 2341 tactagagat ccgtttaggc tgggcggtga tagcttctcg ttcaggcagt acgcctcttt 2401 tcttttccag acctgaggga ggcggaaatg gtgtgaggtt cccggggaaa agccaaatag 2461 gcgatcgcgg gagtgcttta tttgaagatc aggctatcac tgcggtcaat agatttcaca 2521 atgtgatggc tggacagcct gaggaactct cgaacccgaa tggaaacaac cagatattta 2581 tgaatcagcg cggctcacat ggcgttgtgc tggcaaatgc aggttcatcc tctgtctcta 2641 tcaatacggc aacaaaattg cctgatggca ggtatgacaa taaagctgga gcgggttcat 2701 ttcaagtgaa cgatggtaaa ctgacaggca cgatcaatgc caggtctgta gctgtgcttt 2761 atcctgatga tattgcaaaa gcgcctcatg ttttccttga gaattacaaa acaggtgtaa 2821 cacattcttt caatgatcaa ctgacgatta ccttgcgtgc agatgcgaat acaacaaaag 2881 ccgtttatca aatcaataat ggaccagaga cggcgtttaa ggatggagat caattcacaa 2941 tcggaaaagg agatccattt ggcaaaacat acaccatcat gttaaaagga acgaacagtg 3001 atggtgtaac gaggaccgag aaatacagtt ttgttaaaag agatccagcg tcggccaaaa 3061 ccatcggcta tcaaaatccg aatcattgga gccaggtaaa tgcttatatc tataaacatg 3121 atgggagccg agtaattgaa ttgaccggat cttggcctgg aaaaccaatg actaaaaatg 3181 cagacggaat ttacacgctg acgctgcctg cggacacgga tacaaccaac gcaaaagtga 3241 tttttaataa tggcagcgcc caagtgcccg gtcagaatca gcctggcttt gattacgtgc 3301 taaatggttt atataatgac tcgggcttaa gcggttctct tccccattga //