##gff-version 3
##sequence-region BBa_K143080 1 3350
BBa_K143080	.	engineered_region	1498	3350	.	+	0	ID=BBa_K143076;Name=BBa_K143076
BBa_K143080	.	sequence_feature	2349	3350	.	+	0	ID=BBa_K143002;Name=BBa_K143002;Parent=BBa_K143076
BBa_K143080	.	sequence_feature	2349	3353	.	+	0	ID=annotation1974146;Name=3' AmyE homologous sequence;Parent=BBa_K143002
BBa_K143080	.	engineered_region	1498	2340	.	+	0	ID=BBa_J04650;Name=BBa_J04650;Parent=BBa_K143076
BBa_K143080	.	terminator	2300	2340	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04650
BBa_K143080	.	stem_loop	2307	2326	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K143080	.	engineered_region	2300	2340	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K143080	.	stop_codon	2333	2333	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K143080	.	polyA_site	2327	2340	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K143080	.	CDS	1498	2178	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04650
BBa_K143080	.	CDS	1498	2172	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K143080	.	engineered_tag	2179	2203	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K143080	.	terminator	2212	2291	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04650
BBa_K143080	.	stem_loop	2223	2266	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K143080	.	engineered_region	2212	2291	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K143080	.	engineered_region	1	1491	.	+	0	ID=BBa_K143071;Name=BBa_K143071
BBa_K143080	.	promoter	531	586	.	+	0	ID=BBa_K143013;Name=BBa_K143013;Parent=BBa_K143071
BBa_K143080	.	promoter	577	582	.	+	0	ID=annotation1975709;Name=Sigma A -10;Parent=BBa_K143013
BBa_K143080	.	promoter	554	559	.	+	0	ID=annotation1975708;Name=Sigma A -35;Parent=BBa_K143013
BBa_K143080	.	promoter	544	552	.	+	0	ID=annotation1975706;Name=Sigma B -35;Parent=BBa_K143013
BBa_K143080	.	promoter	568	577	.	+	0	ID=annotation1975707;Name=Sigma B -10;Parent=BBa_K143013
BBa_K143080	.	CDS	612	1271	.	+	0	ID=BBa_J31005;Name=BBa_J31005;Parent=BBa_K143071
BBa_K143080	.	sequence_feature	612	1271	.	+	0	ID=annotation1884999;Name=CmR gene;Parent=BBa_J31005
BBa_K143080	.	terminator	1280	1359	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K143071
BBa_K143080	.	stem_loop	1291	1334	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K143080	.	engineered_region	1280	1359	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K143080	.	sequence_feature	1	522	.	+	0	ID=BBa_K143001;Name=BBa_K143001;Parent=BBa_K143071
BBa_K143080	.	sequence_feature	1	522	.	+	0	ID=annotation1974145;Name=5' AmyE homologous sequence;Parent=BBa_K143001
BBa_K143080	.	ribosome_entry_site	1481	1491	.	+	0	ID=BBa_K143020;Name=BBa_K143020;Parent=BBa_K143071
BBa_K143080	.	ribosome_entry_site	1482	1488	.	+	0	ID=annotation1975872;Name=rbs;Parent=BBa_K143020
BBa_K143080	.	promoter	1417	1472	.	+	0	ID=BBa_K143013;Name=BBa_K143013;Parent=BBa_K143071
BBa_K143080	.	promoter	1463	1468	.	+	0	ID=annotation1975709;Name=Sigma A -10;Parent=BBa_K143013
BBa_K143080	.	promoter	1440	1445	.	+	0	ID=annotation1975708;Name=Sigma A -35;Parent=BBa_K143013
BBa_K143080	.	promoter	1430	1438	.	+	0	ID=annotation1975706;Name=Sigma B -35;Parent=BBa_K143013
BBa_K143080	.	promoter	1454	1463	.	+	0	ID=annotation1975707;Name=Sigma B -10;Parent=BBa_K143013
BBa_K143080	.	terminator	1368	1408	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K143071
BBa_K143080	.	stem_loop	1375	1394	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K143080	.	engineered_region	1368	1408	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K143080	.	stop_codon	1401	1401	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K143080	.	polyA_site	1395	1408	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K143080	.	ribosome_entry_site	595	605	.	+	0	ID=BBa_K143020;Name=BBa_K143020;Parent=BBa_K143071
BBa_K143080	.	ribosome_entry_site	596	602	.	+	0	ID=annotation1975872;Name=rbs;Parent=BBa_K143020
>BBa_K143080
atgtttgcaaaacgattcaaaacctctttactgccgttattcgctggatttttattgctgtttcatttggttctggcag
gaccggcggctgcgagtgctgaaacggcgaacaaatcgaatgagcttacagcaccgtcgatcaaaagcggaaccattct
tcatgcatggaattggtcgttcaatacgttaaaacacaatatgaaggatattcatgatgcaggatatacagccattcag
acatctccgattaaccaagtaaaggaagggaatcaaggagataaaagcatgtcgaactggtactggctgtatcagccga
catcgtatcaaattggcaaccgttacttaggtactgaacaagaatttaaagaaatgtgtgcagccgctgaagaatatgg
cataaaggtcattgttgacgcggtcatcaatcataccaccagtgattatgccgcgatttccaatgaggttaagagtatt
ccaaactggacacatggaaacacacaaattaaaaactggtctgatcgatactagagattttacatttttagaaatgggc
gtgaaaaaaagcgcgcgattatgtaaaatataatactagagtaaaggaggaatactagatggagaaaaaaatcactgga
tataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctata
accagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttat
tcacattcttgcccgcctgatgaatgctcatccggaatttcgtatggcaatgaaagacggtgagctggtgatatgggat
agtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaataccacgacgatt
tccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttat
tgagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaac
ttcttcgcccccgttttcaccatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgattcaggttc
atcatgccgtttgtgatggcttccatgtcggcagaatgcttaatgaattacaacagtactgcgatgagtggcagggcgg
ggcgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgttt
gtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagatttta
catttttagaaatgggcgtgaaaaaaagcgcgcgattatgtaaaatataatactagagtaaaggaggaatactagatgg
cttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcga
aatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctg
ccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccgg
actacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgt
tacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggt
ccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtg
aaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaacc
ggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaa
cagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagc
caggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
tactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagatccgtttaggctgggcggtga
tagcttctcgttcaggcagtacgcctcttttcttttccagacctgagggaggcggaaatggtgtgaggttcccggggaa
aagccaaataggcgatcgcgggagtgctttatttgaagatcaggctatcactgcggtcaatagatttcacaatgtgatg
gctggacagcctgaggaactctcgaacccgaatggaaacaaccagatatttatgaatcagcgcggctcacatggcgttg
tgctggcaaatgcaggttcatcctctgtctctatcaatacggcaacaaaattgcctgatggcaggtatgacaataaagc
tggagcgggttcatttcaagtgaacgatggtaaactgacaggcacgatcaatgccaggtctgtagctgtgctttatcct
gatgatattgcaaaagcgcctcatgttttccttgagaattacaaaacaggtgtaacacattctttcaatgatcaactga
cgattaccttgcgtgcagatgcgaatacaacaaaagccgtttatcaaatcaataatggaccagagacggcgtttaagga
tggagatcaattcacaatcggaaaaggagatccatttggcaaaacatacaccatcatgttaaaaggaacgaacagtgat
ggtgtaacgaggaccgagaaatacagttttgttaaaagagatccagcgtcggccaaaaccatcggctatcaaaatccga
atcattggagccaggtaaatgcttatatctataaacatgatgggagccgagtaattgaattgaccggatcttggcctgg
aaaaccaatgactaaaaatgcagacggaatttacacgctgacgctgcctgcggacacggatacaaccaacgcaaaagtg
atttttaataatggcagcgcccaagtgcccggtcagaatcagcctggctttgattacgtgctaaatggtttatataatg
actcgggcttaagcggttctcttccccattga