LOCUS BBa_K143081 3352 bp DNA linear 25-Nov-2024 DEFINITION P43-spoVG RFP expression construct ACCESSION BBa_K143081 VERSION BBa_K143081.1 FEATURES Location/Qualifiers misc_feature 1..522 /label=5' AmyE homologous sequence misc_feature 1..522 /label=BBa_K143001 promoter 544..552 /label=Sigma B -35 promoter 568..577 /label=Sigma B -10 promoter 554..559 /label=Sigma A -35 promoter 577..582 /label=Sigma A -10 promoter 531..586 /label=BBa_K143013 RBS 595..606 /label=rbs RBS 595..606 /label=BBa_K143021 misc_feature 613..1272 /label=CmR gene CDS 613..1272 /label=BBa_J31005 stem_loop 1292..1335 /label=stem_loop misc_feature 1281..1360 /label=BBa_B0010 terminator 1281..1360 /label=BBa_B0010 stem_loop 1376..1395 /label=T7 TE misc_feature 1402..1402 /label=stop polyA_site 1396..1409 /label=polya misc_feature 1369..1409 /label=BBa_B0012 terminator 1369..1409 /label=BBa_B0012 promoter 1431..1439 /label=Sigma B -35 promoter 1455..1464 /label=Sigma B -10 promoter 1441..1446 /label=Sigma A -35 promoter 1464..1469 /label=Sigma A -10 promoter 1418..1473 /label=BBa_K143013 RBS 1482..1493 /label=rbs RBS 1482..1493 /label=BBa_K143021 misc_feature 1..1493 /label=BBa_K143072 CDS 1500..2174 /label=mrfp1 misc_feature 2181..2205 /label=Help:Barcodes CDS 1500..2180 /label=BBa_E1010 stem_loop 2225..2268 /label=stem_loop misc_feature 2214..2293 /label=BBa_B0010 terminator 2214..2293 /label=BBa_B0010 stem_loop 2309..2328 /label=T7 TE misc_feature 2335..2335 /label=stop polyA_site 2329..2342 /label=polya misc_feature 2302..2342 /label=BBa_B0012 terminator 2302..2342 /label=BBa_B0012 misc_feature 1500..2342 /label=BBa_J04650 misc_feature 2351..3355 /label=3' AmyE homologous sequence misc_feature 2351..3352 /label=BBa_K143002 misc_feature 1500..3352 /label=BBa_K143076 ORIGIN 1 atgtttgcaa aacgattcaa aacctcttta ctgccgttat tcgctggatt tttattgctg 61 tttcatttgg ttctggcagg accggcggct gcgagtgctg aaacggcgaa caaatcgaat 121 gagcttacag caccgtcgat caaaagcgga accattcttc atgcatggaa ttggtcgttc 181 aatacgttaa aacacaatat gaaggatatt catgatgcag gatatacagc cattcagaca 241 tctccgatta accaagtaaa ggaagggaat caaggagata aaagcatgtc gaactggtac 301 tggctgtatc agccgacatc gtatcaaatt ggcaaccgtt acttaggtac tgaacaagaa 361 tttaaagaaa tgtgtgcagc cgctgaagaa tatggcataa aggtcattgt tgacgcggtc 421 atcaatcata ccaccagtga ttatgccgcg atttccaatg aggttaagag tattccaaac 481 tggacacatg gaaacacaca aattaaaaac tggtctgatc gatactagag attttacatt 541 tttagaaatg ggcgtgaaaa aaagcgcgcg attatgtaaa atataatact agagaaaggt 601 ggtgaatact agatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg 661 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc 721 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat 781 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt tcgtatggca 841 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat 901 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt 961 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa 1021 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt 1081 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat 1141 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt 1201 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag 1261 ggcggggcgt aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg 1321 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc 1381 accttcgggt gggcctttct gcgtttatat actagagatt ttacattttt agaaatgggc 1441 gtgaaaaaaa gcgcgcgatt atgtaaaata taatactaga gaaaggtggt gaatactaga 1501 tggcttcctc cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt 1561 ccgttaacgg tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta 1621 cccagaccgc taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc 1681 tgtccccgca gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg 1741 actacctgaa actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag 1801 acggtggtgt tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca 1861 aagttaaact gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca 1921 tgggttggga agcttccacc gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa 1981 tcaaaatgcg tctgaaactg aaagacggtg gtcactacga cgctgaagtt aaaaccacct 2041 acatggctaa aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc aaactggaca 2101 tcacctccca caacgaagac tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc 2161 actccaccgg tgcttaataa cgctgatagt gctagtgtag atcgctacta gagccaggca 2221 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc 2281 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta 2341 tatactagag atccgtttag gctgggcggt gatagcttct cgttcaggca gtacgcctct 2401 tttcttttcc agacctgagg gaggcggaaa tggtgtgagg ttcccgggga aaagccaaat 2461 aggcgatcgc gggagtgctt tatttgaaga tcaggctatc actgcggtca atagatttca 2521 caatgtgatg gctggacagc ctgaggaact ctcgaacccg aatggaaaca accagatatt 2581 tatgaatcag cgcggctcac atggcgttgt gctggcaaat gcaggttcat cctctgtctc 2641 tatcaatacg gcaacaaaat tgcctgatgg caggtatgac aataaagctg gagcgggttc 2701 atttcaagtg aacgatggta aactgacagg cacgatcaat gccaggtctg tagctgtgct 2761 ttatcctgat gatattgcaa aagcgcctca tgttttcctt gagaattaca aaacaggtgt 2821 aacacattct ttcaatgatc aactgacgat taccttgcgt gcagatgcga atacaacaaa 2881 agccgtttat caaatcaata atggaccaga gacggcgttt aaggatggag atcaattcac 2941 aatcggaaaa ggagatccat ttggcaaaac atacaccatc atgttaaaag gaacgaacag 3001 tgatggtgta acgaggaccg agaaatacag ttttgttaaa agagatccag cgtcggccaa 3061 aaccatcggc tatcaaaatc cgaatcattg gagccaggta aatgcttata tctataaaca 3121 tgatgggagc cgagtaattg aattgaccgg atcttggcct ggaaaaccaa tgactaaaaa 3181 tgcagacgga atttacacgc tgacgctgcc tgcggacacg gatacaacca acgcaaaagt 3241 gatttttaat aatggcagcg cccaagtgcc cggtcagaat cagcctggct ttgattacgt 3301 gctaaatggt ttatataatg actcgggctt aagcggttct cttccccatt ga //