##gff-version 3
##sequence-region BBa_K143081 1 3352
BBa_K143081	.	engineered_region	1500	3352	.	+	0	ID=BBa_K143076;Name=BBa_K143076
BBa_K143081	.	sequence_feature	2351	3352	.	+	0	ID=BBa_K143002;Name=BBa_K143002;Parent=BBa_K143076
BBa_K143081	.	sequence_feature	2351	3355	.	+	0	ID=annotation1974146;Name=3' AmyE homologous sequence;Parent=BBa_K143002
BBa_K143081	.	engineered_region	1500	2342	.	+	0	ID=BBa_J04650;Name=BBa_J04650;Parent=BBa_K143076
BBa_K143081	.	terminator	2214	2293	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04650
BBa_K143081	.	stem_loop	2225	2268	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K143081	.	engineered_region	2214	2293	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K143081	.	terminator	2302	2342	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04650
BBa_K143081	.	engineered_region	2302	2342	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K143081	.	stop_codon	2335	2335	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K143081	.	stem_loop	2309	2328	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K143081	.	polyA_site	2329	2342	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K143081	.	CDS	1500	2180	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04650
BBa_K143081	.	CDS	1500	2174	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K143081	.	engineered_tag	2181	2205	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K143081	.	engineered_region	1	1493	.	+	0	ID=BBa_K143072;Name=BBa_K143072
BBa_K143081	.	terminator	1369	1409	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K143072
BBa_K143081	.	engineered_region	1369	1409	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K143081	.	stop_codon	1402	1402	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K143081	.	stem_loop	1376	1395	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K143081	.	polyA_site	1396	1409	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K143081	.	promoter	1418	1473	.	+	0	ID=BBa_K143013;Name=BBa_K143013;Parent=BBa_K143072
BBa_K143081	.	promoter	1431	1439	.	+	0	ID=annotation1975706;Name=Sigma B -35;Parent=BBa_K143013
BBa_K143081	.	promoter	1464	1469	.	+	0	ID=annotation1975709;Name=Sigma A -10;Parent=BBa_K143013
BBa_K143081	.	promoter	1455	1464	.	+	0	ID=annotation1975707;Name=Sigma B -10;Parent=BBa_K143013
BBa_K143081	.	promoter	1441	1446	.	+	0	ID=annotation1975708;Name=Sigma A -35;Parent=BBa_K143013
BBa_K143081	.	CDS	613	1272	.	+	0	ID=BBa_J31005;Name=BBa_J31005;Parent=BBa_K143072
BBa_K143081	.	sequence_feature	613	1272	.	+	0	ID=annotation1884999;Name=CmR gene;Parent=BBa_J31005
BBa_K143081	.	promoter	531	586	.	+	0	ID=BBa_K143013;Name=BBa_K143013;Parent=BBa_K143072
BBa_K143081	.	promoter	544	552	.	+	0	ID=annotation1975706;Name=Sigma B -35;Parent=BBa_K143013
BBa_K143081	.	promoter	577	582	.	+	0	ID=annotation1975709;Name=Sigma A -10;Parent=BBa_K143013
BBa_K143081	.	promoter	568	577	.	+	0	ID=annotation1975707;Name=Sigma B -10;Parent=BBa_K143013
BBa_K143081	.	promoter	554	559	.	+	0	ID=annotation1975708;Name=Sigma A -35;Parent=BBa_K143013
BBa_K143081	.	terminator	1281	1360	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K143072
BBa_K143081	.	stem_loop	1292	1335	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K143081	.	engineered_region	1281	1360	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K143081	.	ribosome_entry_site	1482	1493	.	+	0	ID=BBa_K143021;Name=BBa_K143021;Parent=BBa_K143072
BBa_K143081	.	ribosome_entry_site	1482	1493	.	+	0	ID=annotation1975997;Name=rbs;Parent=BBa_K143021
BBa_K143081	.	ribosome_entry_site	595	606	.	+	0	ID=BBa_K143021;Name=BBa_K143021;Parent=BBa_K143072
BBa_K143081	.	ribosome_entry_site	595	606	.	+	0	ID=annotation1975997;Name=rbs;Parent=BBa_K143021
BBa_K143081	.	sequence_feature	1	522	.	+	0	ID=BBa_K143001;Name=BBa_K143001;Parent=BBa_K143072
BBa_K143081	.	sequence_feature	1	522	.	+	0	ID=annotation1974145;Name=5' AmyE homologous sequence;Parent=BBa_K143001
>BBa_K143081
atgtttgcaaaacgattcaaaacctctttactgccgttattcgctggatttttattgctgtttcatttggttctggcag
gaccggcggctgcgagtgctgaaacggcgaacaaatcgaatgagcttacagcaccgtcgatcaaaagcggaaccattct
tcatgcatggaattggtcgttcaatacgttaaaacacaatatgaaggatattcatgatgcaggatatacagccattcag
acatctccgattaaccaagtaaaggaagggaatcaaggagataaaagcatgtcgaactggtactggctgtatcagccga
catcgtatcaaattggcaaccgttacttaggtactgaacaagaatttaaagaaatgtgtgcagccgctgaagaatatgg
cataaaggtcattgttgacgcggtcatcaatcataccaccagtgattatgccgcgatttccaatgaggttaagagtatt
ccaaactggacacatggaaacacacaaattaaaaactggtctgatcgatactagagattttacatttttagaaatgggc
gtgaaaaaaagcgcgcgattatgtaaaatataatactagagaaaggtggtgaatactagatggagaaaaaaatcactgg
atataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctat
aaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggccttta
ttcacattcttgcccgcctgatgaatgctcatccggaatttcgtatggcaatgaaagacggtgagctggtgatatggga
tagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaataccacgacgat
ttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggttta
ttgagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaa
cttcttcgcccccgttttcaccatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgattcaggtt
catcatgccgtttgtgatggcttccatgtcggcagaatgcttaatgaattacaacagtactgcgatgagtggcagggcg
gggcgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtt
tgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagatttt
acatttttagaaatgggcgtgaaaaaaagcgcgcgattatgtaaaatataatactagagaaaggtggtgaatactagat
ggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttc
gaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgc
tgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatccc
ggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttacc
gttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacg
gtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaagg
tgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaa
ccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttg
aacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagatccgtttaggctgggcggt
gatagcttctcgttcaggcagtacgcctcttttcttttccagacctgagggaggcggaaatggtgtgaggttcccgggg
aaaagccaaataggcgatcgcgggagtgctttatttgaagatcaggctatcactgcggtcaatagatttcacaatgtga
tggctggacagcctgaggaactctcgaacccgaatggaaacaaccagatatttatgaatcagcgcggctcacatggcgt
tgtgctggcaaatgcaggttcatcctctgtctctatcaatacggcaacaaaattgcctgatggcaggtatgacaataaa
gctggagcgggttcatttcaagtgaacgatggtaaactgacaggcacgatcaatgccaggtctgtagctgtgctttatc
ctgatgatattgcaaaagcgcctcatgttttccttgagaattacaaaacaggtgtaacacattctttcaatgatcaact
gacgattaccttgcgtgcagatgcgaatacaacaaaagccgtttatcaaatcaataatggaccagagacggcgtttaag
gatggagatcaattcacaatcggaaaaggagatccatttggcaaaacatacaccatcatgttaaaaggaacgaacagtg
atggtgtaacgaggaccgagaaatacagttttgttaaaagagatccagcgtcggccaaaaccatcggctatcaaaatcc
gaatcattggagccaggtaaatgcttatatctataaacatgatgggagccgagtaattgaattgaccggatcttggcct
ggaaaaccaatgactaaaaatgcagacggaatttacacgctgacgctgcctgcggacacggatacaaccaacgcaaaag
tgatttttaataatggcagcgcccaagtgcccggtcagaatcagcctggctttgattacgtgctaaatggtttatataa
tgactcgggcttaagcggttctcttccccattga