LOCUS BBa_K143082 3434 bp DNA linear 29-Jun-2024 DEFINITION Pveg-spoVG RFP expression construct ACCESSION BBa_K143082 VERSION BBa_K143082.1 FEATURES Location/Qualifiers misc_feature 1..522 /label=5' AmyE homologous sequence misc_feature 1..522 /label=BBa_K143001 promoter 593..598 /label=Sigma A-35 promoter 616..621 /label=Sigma A -10 promoter 531..627 /label=BBa_K143012 RBS 636..647 /label=rbs RBS 636..647 /label=BBa_K143021 misc_feature 654..1313 /label=CmR gene CDS 654..1313 /label=BBa_J31005 stem_loop 1333..1376 /label=stem_loop misc_feature 1322..1401 /label=BBa_B0010 terminator 1322..1401 /label=BBa_B0010 stem_loop 1417..1436 /label=T7 TE misc_feature 1443..1443 /label=stop polyA_site 1437..1450 /label=polya misc_feature 1410..1450 /label=BBa_B0012 terminator 1410..1450 /label=BBa_B0012 promoter 1521..1526 /label=Sigma A-35 promoter 1544..1549 /label=Sigma A -10 promoter 1459..1555 /label=BBa_K143012 RBS 1564..1575 /label=rbs RBS 1564..1575 /label=BBa_K143021 misc_feature 1..1575 /label=BBa_K143074 CDS 1582..2256 /label=mrfp1 misc_feature 2263..2287 /label=Help:Barcodes CDS 1582..2262 /label=BBa_E1010 stem_loop 2307..2350 /label=stem_loop misc_feature 2296..2375 /label=BBa_B0010 terminator 2296..2375 /label=BBa_B0010 stem_loop 2391..2410 /label=T7 TE misc_feature 2417..2417 /label=stop polyA_site 2411..2424 /label=polya misc_feature 2384..2424 /label=BBa_B0012 terminator 2384..2424 /label=BBa_B0012 misc_feature 1582..2424 /label=BBa_J04650 misc_feature 2433..3437 /label=3' AmyE homologous sequence misc_feature 2433..3434 /label=BBa_K143002 misc_feature 1582..3434 /label=BBa_K143076 ORIGIN 1 atgtttgcaa aacgattcaa aacctcttta ctgccgttat tcgctggatt tttattgctg 61 tttcatttgg ttctggcagg accggcggct gcgagtgctg aaacggcgaa caaatcgaat 121 gagcttacag caccgtcgat caaaagcgga accattcttc atgcatggaa ttggtcgttc 181 aatacgttaa aacacaatat gaaggatatt catgatgcag gatatacagc cattcagaca 241 tctccgatta accaagtaaa ggaagggaat caaggagata aaagcatgtc gaactggtac 301 tggctgtatc agccgacatc gtatcaaatt ggcaaccgtt acttaggtac tgaacaagaa 361 tttaaagaaa tgtgtgcagc cgctgaagaa tatggcataa aggtcattgt tgacgcggtc 421 atcaatcata ccaccagtga ttatgccgcg atttccaatg aggttaagag tattccaaac 481 tggacacatg gaaacacaca aattaaaaac tggtctgatc gatactagag aattttgtca 541 aaataatttt attgacaacg tcttattaac gttgatataa tttaaatttt atttgacaaa 601 aatgggctcg tgttgtacaa taaatgttac tagagaaagg tggtgaatac tagatggaga 661 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg 721 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg 781 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc 841 ttgcccgcct gatgaatgct catccggaat ttcgtatggc aatgaaagac ggtgagctgg 901 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt 961 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag 1021 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt 1081 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata 1141 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg 1201 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca 1261 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taatactaga 1321 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 1381 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc 1441 tgcgtttata tactagagaa ttttgtcaaa ataattttat tgacaacgtc ttattaacgt 1501 tgatataatt taaattttat ttgacaaaaa tgggctcgtg ttgtacaata aatgttacta 1561 gagaaaggtg gtgaatacta gatggcttcc tccgaagacg ttatcaaaga gttcatgcgt 1621 ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt 1681 gaaggtcgtc cgtacgaagg tacccagacc gctaaactga aagttaccaa aggtggtccg 1741 ctgccgttcg cttgggacat cctgtccccg cagttccagt acggttccaa agcttacgtt 1801 aaacacccgg ctgacatccc ggactacctg aaactgtcct tcccggaagg tttcaaatgg 1861 gaacgtgtta tgaacttcga agacggtggt gttgttaccg ttacccagga ctcctccctg 1921 caagacggtg agttcatcta caaagttaaa ctgcgtggta ccaacttccc gtccgacggt 1981 ccggttatgc agaaaaaaac catgggttgg gaagcttcca ccgaacgtat gtacccggaa 2041 gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac 2101 gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac 2161 aaaaccgaca tcaaactgga catcacctcc cacaacgaag actacaccat cgttgaacag 2221 tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat aacgctgata gtgctagtgt 2281 agatcgctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct 2341 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt 2401 cgggtgggcc tttctgcgtt tatatactag agatccgttt aggctgggcg gtgatagctt 2461 ctcgttcagg cagtacgcct cttttctttt ccagacctga gggaggcgga aatggtgtga 2521 ggttcccggg gaaaagccaa ataggcgatc gcgggagtgc tttatttgaa gatcaggcta 2581 tcactgcggt caatagattt cacaatgtga tggctggaca gcctgaggaa ctctcgaacc 2641 cgaatggaaa caaccagata tttatgaatc agcgcggctc acatggcgtt gtgctggcaa 2701 atgcaggttc atcctctgtc tctatcaata cggcaacaaa attgcctgat ggcaggtatg 2761 acaataaagc tggagcgggt tcatttcaag tgaacgatgg taaactgaca ggcacgatca 2821 atgccaggtc tgtagctgtg ctttatcctg atgatattgc aaaagcgcct catgttttcc 2881 ttgagaatta caaaacaggt gtaacacatt ctttcaatga tcaactgacg attaccttgc 2941 gtgcagatgc gaatacaaca aaagccgttt atcaaatcaa taatggacca gagacggcgt 3001 ttaaggatgg agatcaattc acaatcggaa aaggagatcc atttggcaaa acatacacca 3061 tcatgttaaa aggaacgaac agtgatggtg taacgaggac cgagaaatac agttttgtta 3121 aaagagatcc agcgtcggcc aaaaccatcg gctatcaaaa tccgaatcat tggagccagg 3181 taaatgctta tatctataaa catgatggga gccgagtaat tgaattgacc ggatcttggc 3241 ctggaaaacc aatgactaaa aatgcagacg gaatttacac gctgacgctg cctgcggaca 3301 cggatacaac caacgcaaaa gtgattttta ataatggcag cgcccaagtg cccggtcaga 3361 atcagcctgg ctttgattac gtgctaaatg gtttatataa tgactcgggc ttaagcggtt 3421 ctcttcccca ttga //