##gff-version 3
##sequence-region BBa_K143082 1 3434
BBa_K143082	.	engineered_region	1582	3434	.	+	0	ID=BBa_K143076;Name=BBa_K143076
BBa_K143082	.	engineered_region	1582	2424	.	+	0	ID=BBa_J04650;Name=BBa_J04650;Parent=BBa_K143076
BBa_K143082	.	CDS	1582	2262	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04650
BBa_K143082	.	CDS	1582	2256	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K143082	.	engineered_tag	2263	2287	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K143082	.	terminator	2296	2375	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04650
BBa_K143082	.	engineered_region	2296	2375	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K143082	.	stem_loop	2307	2350	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K143082	.	terminator	2384	2424	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04650
BBa_K143082	.	engineered_region	2384	2424	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K143082	.	stem_loop	2391	2410	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K143082	.	stop_codon	2417	2417	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K143082	.	polyA_site	2411	2424	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K143082	.	sequence_feature	2433	3434	.	+	0	ID=BBa_K143002;Name=BBa_K143002;Parent=BBa_K143076
BBa_K143082	.	sequence_feature	2433	3437	.	+	0	ID=annotation1974146;Name=3' AmyE homologous sequence;Parent=BBa_K143002
BBa_K143082	.	engineered_region	1	1575	.	+	0	ID=BBa_K143074;Name=BBa_K143074
BBa_K143082	.	sequence_feature	1	522	.	+	0	ID=BBa_K143001;Name=BBa_K143001;Parent=BBa_K143074
BBa_K143082	.	sequence_feature	1	522	.	+	0	ID=annotation1974145;Name=5' AmyE homologous sequence;Parent=BBa_K143001
BBa_K143082	.	ribosome_entry_site	636	647	.	+	0	ID=BBa_K143021;Name=BBa_K143021;Parent=BBa_K143074
BBa_K143082	.	ribosome_entry_site	636	647	.	+	0	ID=annotation1975997;Name=rbs;Parent=BBa_K143021
BBa_K143082	.	promoter	531	627	.	+	0	ID=BBa_K143012;Name=BBa_K143012;Parent=BBa_K143074
BBa_K143082	.	promoter	593	598	.	+	0	ID=annotation1975704;Name=Sigma A-35;Parent=BBa_K143012
BBa_K143082	.	promoter	616	621	.	+	0	ID=annotation1975705;Name=Sigma A -10;Parent=BBa_K143012
BBa_K143082	.	terminator	1410	1450	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K143074
BBa_K143082	.	engineered_region	1410	1450	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K143082	.	stem_loop	1417	1436	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K143082	.	stop_codon	1443	1443	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K143082	.	polyA_site	1437	1450	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K143082	.	CDS	654	1313	.	+	0	ID=BBa_J31005;Name=BBa_J31005;Parent=BBa_K143074
BBa_K143082	.	sequence_feature	654	1313	.	+	0	ID=annotation1884999;Name=CmR gene;Parent=BBa_J31005
BBa_K143082	.	terminator	1322	1401	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K143074
BBa_K143082	.	engineered_region	1322	1401	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K143082	.	stem_loop	1333	1376	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K143082	.	promoter	1459	1555	.	+	0	ID=BBa_K143012;Name=BBa_K143012;Parent=BBa_K143074
BBa_K143082	.	promoter	1521	1526	.	+	0	ID=annotation1975704;Name=Sigma A-35;Parent=BBa_K143012
BBa_K143082	.	promoter	1544	1549	.	+	0	ID=annotation1975705;Name=Sigma A -10;Parent=BBa_K143012
BBa_K143082	.	ribosome_entry_site	1564	1575	.	+	0	ID=BBa_K143021;Name=BBa_K143021;Parent=BBa_K143074
BBa_K143082	.	ribosome_entry_site	1564	1575	.	+	0	ID=annotation1975997;Name=rbs;Parent=BBa_K143021
>BBa_K143082
atgtttgcaaaacgattcaaaacctctttactgccgttattcgctggatttttattgctgtttcatttggttctggcag
gaccggcggctgcgagtgctgaaacggcgaacaaatcgaatgagcttacagcaccgtcgatcaaaagcggaaccattct
tcatgcatggaattggtcgttcaatacgttaaaacacaatatgaaggatattcatgatgcaggatatacagccattcag
acatctccgattaaccaagtaaaggaagggaatcaaggagataaaagcatgtcgaactggtactggctgtatcagccga
catcgtatcaaattggcaaccgttacttaggtactgaacaagaatttaaagaaatgtgtgcagccgctgaagaatatgg
cataaaggtcattgttgacgcggtcatcaatcataccaccagtgattatgccgcgatttccaatgaggttaagagtatt
ccaaactggacacatggaaacacacaaattaaaaactggtctgatcgatactagagaattttgtcaaaataattttatt
gacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgttacta
gagaaaggtggtgaatactagatggagaaaaaaatcactggatataccaccgttgatatatcccaatggcatcgtaaag
aacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaa
gaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctcatccggaa
tttcgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgttacaccgttttccatgagcaaa
ctgaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttctacacatatattcgcaagatgtggc
gtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctgggtg
agtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcaccatgggcaaatattata
cgcaaggcgacaaggtgctgatgccgctggcgattcaggttcatcatgccgtttgtgatggcttccatgtcggcagaat
gcttaatgaattacaacagtactgcgatgagtggcagggcggggcgtaatactagagccaggcatcaaataaaacgaaa
ggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctca
ccttcgggtgggcctttctgcgtttatatactagagaattttgtcaaaataattttattgacaacgtcttattaacgtt
gatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgttactagagaaaggtggtgaatacta
gatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgag
ttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtc
cgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacat
cccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgtt
accgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccg
acggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaa
aggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaa
aaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcg
ttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctact
agagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacg
ctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagatccgtttaggctgggc
ggtgatagcttctcgttcaggcagtacgcctcttttcttttccagacctgagggaggcggaaatggtgtgaggttcccg
gggaaaagccaaataggcgatcgcgggagtgctttatttgaagatcaggctatcactgcggtcaatagatttcacaatg
tgatggctggacagcctgaggaactctcgaacccgaatggaaacaaccagatatttatgaatcagcgcggctcacatgg
cgttgtgctggcaaatgcaggttcatcctctgtctctatcaatacggcaacaaaattgcctgatggcaggtatgacaat
aaagctggagcgggttcatttcaagtgaacgatggtaaactgacaggcacgatcaatgccaggtctgtagctgtgcttt
atcctgatgatattgcaaaagcgcctcatgttttccttgagaattacaaaacaggtgtaacacattctttcaatgatca
actgacgattaccttgcgtgcagatgcgaatacaacaaaagccgtttatcaaatcaataatggaccagagacggcgttt
aaggatggagatcaattcacaatcggaaaaggagatccatttggcaaaacatacaccatcatgttaaaaggaacgaaca
gtgatggtgtaacgaggaccgagaaatacagttttgttaaaagagatccagcgtcggccaaaaccatcggctatcaaaa
tccgaatcattggagccaggtaaatgcttatatctataaacatgatgggagccgagtaattgaattgaccggatcttgg
cctggaaaaccaatgactaaaaatgcagacggaatttacacgctgacgctgcctgcggacacggatacaaccaacgcaa
aagtgatttttaataatggcagcgcccaagtgcccggtcagaatcagcctggctttgattacgtgctaaatggtttata
taatgactcgggcttaagcggttctcttccccattga