BBa_K1431302 1 BBa_K1431302 SV40 promoter+SV40 PolyA 2014-10-09T11:00:00Z 2015-05-08T01:10:24Z It is from our instructor's lab. SV40 promoter is a kind of eukaryocyte cell promoters, of course PolyA also needful. There are two BbsI sites, which recognize six bases[GAAGAC] but cut downstream sites between two bases and four bases, between promoter and PolyA which can make the coding sequence seamless gather with them. So that we can also test how many or what kind of nucleotides interval between promoter and coding sequence will make the highest expression efficiency. For example, if we want to test the efficiency of two nucleotides interval, we should design the the primer sequence like GAAGACNN'NN***, the first NN will be any bases, the second NN is the interval bases,and *** is the begining of coding sequence. If you still do not understand, you can search how BbsI work on the internet or connect with us. false false _1809_ 0 22882 9 It's complicated false For BbsI site, we have a series same design sequence. You can get how BbsI site work by google. And the reason why we designed that because we want to make coding sequence seamless gather with promoter and PolyA or any amount nucleotides. That depend on which will make the highest efficiency. Sorry for the limited time, we only test GFP seamless gather with promoter and PolyA but did not get the data which will make the highest efficiency. false Rifei Chen, Yushan Zhang annotation2422988 1 SV40 PolyA range2422988 1 401 531 annotation2422987 1 SV40 promoter range2422987 1 5 370 annotation2422989 1 BbsI site range2422989 1 377 382 annotation2422990 1 BbsI site range2422990 1 393 398 BBa_K1431302_sequence 1 gcgaactgtggaatgtgtgtcagttagggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcctctgcctctgagctattccagaagtagtgaggaggcttttttggaggcctaggcttttgcaaaaagctcccgggagcttgtatatccattttcgtcgtcttcatggatctatgaagacccaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgacctctcaattcatcactctcggcatgga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z