BBa_K1433003 1 BBa_K1433003 RBS-gp47-tag-terminator 2014-09-27T11:00:00Z 2015-05-08T01:10:25Z No Bxb1 gp47 is an excisionase and Bxb1 gp35 is a serine integrase in Mycobacterium phage Bxb1. This intergrase along can typically catalyzes site-specific recombination and mediate DNA inversion from an BP to LR state. Heterologous expression of Bxb1 integrase gp35 and excisionase gp47 could mediate DNA restore from an LR to BP state. This part is composed of 4 elements. 1. Promoter: BBa_J23110, a middle-ground Bacterial constitutive promoter. 2. gp47: An excisionase in Mycobacterium phage Bxb1. 3. tag: A distinct proteolysis tag on gp47 peptides. 4. terminator: BBa_B0015, a strong double terminator. This part has component encodes a distinct proteolysis tag on gp47 peptides, for fine control over gp47 synthesis and degradation. false false _1811_ 0 20519 9 It's complicated false No false Hanqing Liu annotation2412271 1 BBa_B0015 Terminator range2412271 1 842 970 annotation2412269 1 Bxb1 Excisonase gp47 range2412269 1 26 802 annotation2412270 1 AAK tag range2412270 1 803 841 annotation2412268 1 RBS range2412268 1 1 25 BBa_K1433003_sequence 1 accttaacaaactacggaggtcaacatggccggcactcagcgtatcgtctttctacccgatactcagttgcctttcgaggcgcgcaaagagatgcaagcggtcatccgcttcatcggggatgtccagccgtacggcgtggtacatatcggtgacgtcctagacctgccgcagccctcgcgctggaaccggggaaccaagggcgagttcgagggctcggtgtaccgcgacgcggactacgccaagaagaacctgatggagccgctgcgcaaggtctacgacggctggatcgggatgcacgagggcaaccacgatctgcgagcccgcgagtacctggccaagaacgcaccggccctggagggtacgcacgctttcgacatcgacgtgctgctcgacttcgacgggttcggtgtggagctgctgcctgacttctacgacatcgctccgggctggatctccactcacgggcacatgggcaagatgacgctatcccagatcgccggatcgacagcgctcaacggtgccaagaagttcggcaagtccgtggtctgcggccacacgcaccggcaggctgtcgtctcgcactcgttcgggtacggcggctcggtgcgcaagaccgtcaccggcatggaagtcgggcacctgatggacatgaagaaggccaactatctaaagggcggagccgggaactggcagatgggcttcgggatgctcacggtcgacggcaagcatgtcaaggctgagatcgtcccgatcctgggaggcaagttcaccgttgacggccaggtctgggaagtcaccggtgcagcaaacgacgaaaactacgctgctgctaaataataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z