LOCUS       BBa_K1433011            1897 bp    DNA     linear       26-Apr-2024
DEFINITION  Terminator-RFP-RBS-attB-P-attP-RBS-GFP-Terminator
ACCESSION   BBa_K1433011
VERSION     BBa_K1433011.1  
FEATURES             Location/Qualifiers
     stem_loop       8..27
                     /label=T7 TE
     misc_feature    34..34
                     /label=stop
     polyA_site      28..41
                     /label=polya
     misc_feature    1..41
                     /label=BBa_B0012
     terminator      1..41
                     /label=BBa_B0012
     stem_loop       61..104
                     /label=stem_loop
     misc_feature    50..129
                     /label=BBa_B0010
     terminator      50..129
                     /label=BBa_B0010
     CDS             136..846
                     /label=mCherry
     misc_feature    487..487
                     /label=C->T (removing PstI site)
     misc_feature    136..849
                     /label=BBa_J06504
     misc_feature    862..865
                     /label=conserved
     RBS             858..869
                     /label=BBa_B0034
     misc_feature    878..926
                     /label=attB
     promoter        927..961
                     /label=J23110 Promoter
     misc_feature    962..1014
                     /label=attP
     misc_feature    878..1014
                     /label=BBa_K1433001
     misc_feature    1027..1030
                     /label=conserved
     RBS             1023..1034
                     /label=BBa_B0034
     CDS             1041..1760
                     /label=GFP protein
     CDS             1041..1760
                     /label=BBa_E0040
     stem_loop       1780..1823
                     /label=stem_loop
     misc_feature    1769..1848
                     /label=BBa_B0010
     terminator      1769..1848
                     /label=BBa_B0010
     stem_loop       1864..1883
                     /label=T7 TE
     misc_feature    1890..1890
                     /label=stop
     polyA_site      1884..1897
                     /label=polya
     misc_feature    1857..1897
                     /label=BBa_B0012
     terminator      1857..1897
                     /label=BBa_B0012
ORIGIN
        1 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagagc caggcatcaa
       61 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
      121 aacgctctct actagatggt gagcaagggc gaggaggata acatggccat catcaaggag
      181 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag
      241 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
      301 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag
      361 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc
      421 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
      481 tcctccttgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc
      541 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg
      601 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc
      661 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
      721 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc
      781 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
      841 aagtaataat actagagaaa gaggagaaat actagagcgg ccggcttgtc gacgacggcg
      901 gtctccgtcg tcaggatcat ccgggcttta cggctagctc agtcctaggt acaatgctag
      961 cgggtttgta ccgtacacca ctgagaccgc ggtggttgac cagacaaacc acgatactag
     1021 agaaagagga gaaatactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa
     1081 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg
     1141 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac
     1201 ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat
     1261 acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac
     1321 aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt
     1381 ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg
     1441 gaaacattct tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg
     1501 cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg
     1561 gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc
     1621 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa
     1681 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg
     1741 atgaactata caaataataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
     1801 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     1861 actggctcac cttcgggtgg gcctttctgc gtttata
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