LOCUS BBa_K1433013 5966 bp DNA linear 24-Apr-2024 DEFINITION rT-rRFP-rRBS-attB-P-attP-RBS-lambda red-RBS-GFP-T-P-RBS-gp47-tag-T ACCESSION BBa_K1433013 VERSION BBa_K1433013.1 FEATURES Location/Qualifiers stem_loop 1..129 /label=BBa_B0015 Terminator CDS 130..857 /label=BBa_J06504 mCherry RBS 858..869 /label=BBa_B0034 RBS misc_feature 870..919 /label=attB promoter 920..954 /label=BBa_J23110 promoter misc_feature 955..1007 /label=attP RBS 1008..1026 /label=RBS CDS 1027..1443 /label=GAM-CDS CDS 1449..2234 /label=Beta-CDS CDS 2231..2911 /label=EXO-CDS RBS 2912..2924 /label=BBa_B0034 RBS CDS 2925..3657 /label=BBa_E0040 GFP stem_loop 3658..3786 /label=BBa_B0015 Terminator promoter 3787..4996 /label=BBa_I0500 Inducible pBad/araC promoter RBS 4997..5021 /label=RBS CDS 5022..5797 /label=BBa_K1433004 Bxb1 excisionase gp447 misc_feature 5799..5835 /label=AAK tag stem_loop 5835..5966 /label=BBa_B0015 Terminator misc_feature 870..1007 /label=BBa_K1433001 misc_feature 1008..2911 /label=BBa_K1433005 ORIGIN 1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca 61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt 121 gatgcctggc tctagtatta ttacttgtac agctcgtcca tgccgccggt ggagtggcgg 181 ccctcggcgc gttcgtactg ttccacgatg gtgtagtcct cgttgtggga ggtgatgtcc 241 aacttgatgt tgacgttgta ggcgccgggc agctgcacgg gcttcttggc cttgtaggtg 301 gtcttgacct cagcgtcgta gtggccgccg tccttcagct tcagcctctg cttgatctcg 361 cccttcaggg cgccgtcctc ggggtacatc cgctcggagg aggcctccca gcccatggtc 421 ttcttctgca ttacggggcc gtcggagggg aagttggtgc cgcgcagctt caccttgtag 481 atgaactcgc cgtcctgcaa ggaggagtcc tgggtcacgg tcaccacgcc gccgtcctcg 541 aagttcatca cgcgctccca cttgaagccc tcggggaagg acagcttcaa gtagtcgggg 601 atgtcggcgg ggtgcttcac gtaggccttg gagccgtaca tgaactgagg ggacaggatg 661 tcccaggcga agggcagggg gccacccttg gtcaccttca gcttggcggt ctgggtgccc 721 tcgtaggggc ggccctcgcc ctcgccctcg atctcgaact cgtggccgtt cacggagccc 781 tccatgtgca ccttgaagcg catgaactcc ttgatgatgg ccatgttatc ctcctcgccc 841 ttgctcacca tctagtattt ctcctctttt cggccggctt gtcgacgacg gcggtctccg 901 tcgtcaggat catccgggct ttacggctag ctcagtccta ggtacaatgc tagcgggttt 961 gtaccgtaca ccactgagac cgcggtggtt gaccagacaa accacgatct aaggaggtta 1021 taaaaaatgg atattaatac tgaaactgag atcaagcaaa agcattcact aacccccttt 1081 cctgttttcc taatcagccc ggcatttcgc gggcgatatt ttcacagcta tttcaggagt 1141 tcagccatga acgcttatta cattcaggat cgtcttgagg ctcagagctg ggcgcgtcac 1201 taccagcagc tcgcccgtga agagaaagag gcagaactgg cagacgacat ggaaaaaggc 1261 ctgccccagc acctgtttga atcgctatgc atcgatcatt tgcaacgcca cggggccagc 1321 aaaaaatcca ttacccgtgc gtttgatgac gatgttgagt ttcaggagcg catggcagaa 1381 cacatccggt acatggttga aaccattgct caccaccagg ttgatattga ttcagaggta 1441 taaaacgaat gagtactgca ctcgcaacgc tggctgggaa gctggctgaa cgtgtcggca 1501 tggattctgt cgacccacag gaactgatca ccactcttcg ccagacggca tttaaaggtg 1561 atgccagcga tgcgcagttc atcgcattac tgatcgttgc caaccagtac ggccttaatc 1621 cgtggacgaa agaaatttac gcctttcctg ataagcagaa tggcatcgtt ccggtggtgg 1681 gcgttgatgg ctggtcccgc atcatcaatg aaaaccagca gtttgatggc atggactttg 1741 agcaggacaa tgaatcctgt acatgccgga tttaccgcaa ggaccgtaat catccgatct 1801 gcgttaccga atggatggat gaatgccgcc gcgaaccatt caaaactcgc gaaggcagag 1861 aaatcacggg gccgtggcag tcgcatccca aacggatgtt acgtcataaa gccatgattc 1921 agtgtgcccg tctggccttc ggatttgctg gtatctatga caaggatgaa gccgagcgca 1981 ttgtcgaaaa tactgcatac acggcagaac gtcagccgga acgcgacatc actccggtta 2041 acgatgaaac catgcaggag attaacactc tgctgatcgc cctggataaa acatgggatg 2101 acgacttatt gccgctctgt tcccagatat ttcgccgcga cattcgtgca tcgtcagaac 2161 tgacacaggc cgaagcagta aaagctcttg gattcctgaa acagaaagcc gcagagcaga 2221 aggtggcagc atgacaccgg acattatcct gcaacgtacc gggatcgatg tgagagctgt 2281 cgaacagggg gatgatgcgt ggcacaaatt acggctcggc gtcatcaccg cttcagaagt 2341 tcacaacgtg atagcaaaac cccgctccgg aaagaagtgg cctgacatga aaatgtccta 2401 cttccacacc ctgcttgctg aggtttgcac cggtgtggct ccggaagtta acgctaaagc 2461 actggcctgg ggaaaacagt acgagaacga cgccagaacc ctgtttgagt tcacttccgg 2521 cgtgaatgtt actgaatccc cgatcatcta tcgcgacgaa agtatgcgta ccgcctgctc 2581 tcccgatggt ttatgcagtg acggcaacgg ccttgaactg aaatgcccgt ttacctcccg 2641 ggatttcatg aagttccggc tcggtggttt cgaggccata aagtcagctt acatggccca 2701 ggtgcagtac agcatgtggg tgacgcgaaa aaatgcctgg tactttgcca actatgaccc 2761 gcgtatgaag cgtgaaggcc tgcattatgt cgtgattgag cgggatgaaa agtacatggc 2821 gagttttgac gagatcgtgc cggagttcat cgaaaaaatg gacgaggcac tggctgaaat 2881 tggttttgta tttggggagc aatggcgatg aaaagaggag aaatactaga tgcgtaaagg 2941 agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg 3001 gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct 3061 taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt 3121 cggttatggt gttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt 3181 caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg 3241 gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga 3301 gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa 3361 ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa 3421 cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca 3481 aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca 3541 atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt 3601 aacagctgct gggattacac atggcatgga tgaactatac aaataataat actagagcca 3661 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 3721 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 3781 tttatattat gacaacttga cggctacatc attcactttt tcttcacaac cggcacggaa 3841 ctcgctcggg ctggccccgg tgcatttttt aaatacccgc gagaaataga gttgatcgtc 3901 aaaaccaaca ttgcgaccga cggtggcgat aggcatccgg gtggtgctca aaagcagctt 3961 cgcctggctg atacgttggt cctcgcgcca gcttaagacg ctaatcccta actgctggcg 4021 gaaaagatgt gacagacgcg acggcgacaa gcaaacatgc tgtgcgacgc tggcgatatc 4081 aaaattgctg tctgccaggt gatcgctgat gtactgacaa gcctcgcgta cccgattatc 4141 catcggtgga tggagcgact cgttaatcgc ttccatgcgc cgcagtaaca attgctcaag 4201 cagatttatc gccagcagct ccgaatagcg cccttcccct tgcccggcgt taatgatttg 4261 cccaaacagg tcgctgaaat gcggctggtg cgcttcatcc gggcgaaaga accccgtatt 4321 ggcaaatatt gacggccagt taagccattc atgccagtag gcgcgcggac gaaagtaaac 4381 ccactggtga taccattcgc gagcctccgg atgacgaccg tagtgatgaa tctctcctgg 4441 cgggaacagc aaaatatcac ccggtcggca aacaaattct cgtccctgat ttttcaccac 4501 cccctgaccg cgaatggtga gattgagaat ataacctttc attcccagcg gtcggtcgat 4561 aaaaaaatcg agataaccgt tggcctcaat cggcgttaaa cccgccacca gatgggcatt 4621 aaacgagtat cccggcagca ggggatcatt ttgcgcttca gccatacttt tcatactccc 4681 gccattcaga gaagaaacca attgtccata ttgcatcaga cattgccgtc actgcgtctt 4741 ttactggctc ttctcgctaa ccaaaccggt aaccccgctt attaaaagca ttctgtaaca 4801 aagcgggacc aaagccatga caaaaacgcg taacaaaagt gtctataatc acggcagaaa 4861 agtccacatt gattatttgc acggcgtcac actttgctat gccatagcat ttttatccat 4921 aagattagcg gatcctacct gacgcttttt atcgcaactc tctactgttt ctccataccc 4981 gtttttttgg gctagcacct taacaaacta cggaggtcaa catggccggc actcagcgta 5041 tcgtctttct acccgatact cagttgcctt tcgaggcgcg caaagagatg caagcggtca 5101 tccgcttcat cggggatgtc cagccgtacg gcgtggtaca tatcggtgac gtcctagacc 5161 tgccgcagcc ctcgcgctgg aaccggggaa ccaagggcga gttcgagggc tcggtgtacc 5221 gcgacgcgga ctacgccaag aagaacctga tggagccgct gcgcaaggtc tacgacggct 5281 ggatcgggat gcacgagggc aaccacgatc tgcgagcccg cgagtacctg gccaagaacg 5341 caccggccct ggagggtacg cacgctttcg acatcgacgt gctgctcgac ttcgacgggt 5401 tcggtgtgga gctgctgcct gacttctacg acatcgctcc gggctggatc tccactcacg 5461 ggcacatggg caagatgacg ctatcccaga tcgccggatc gacagcgctc aacggtgcca 5521 agaagttcgg caagtccgtg gtctgcggcc acacgcaccg gcaggctgtc gtctcgcact 5581 cgttcgggta cggcggctcg gtgcgcaaga ccgtcaccgg catggaagtc gggcacctga 5641 tggacatgaa gaaggccaac tatctaaagg gcggagccgg gaactggcag atgggcttcg 5701 ggatgctcac ggtcgacggc aagcatgtca aggctgagat cgtcccgatc ctgggaggca 5761 agttcaccgt tgacggccag gtctgggaag tcaccggtgc agcaaacgac gaaaactacg 5821 ctgctgctaa ataataacca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 5881 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc 5941 ttcgggtggg cctttctgcg tttata //