LOCUS       BBa_K1433016            2083 bp    DNA     linear       17-May-2024
DEFINITION  P-C-attL-rT-rT-attR-B-B0031-gp35-T
ACCESSION   BBa_K1433016
VERSION     BBa_K1433016.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=J23110 Promoter
     misc_feature    36..75
                     /label=homologous sequence C
     misc_feature    76..125
                     /label=attL
     stem_loop       126..230
                     /label=BBa_B0015 Terminator
     stem_loop       231..335
                     /label=BBa_B0015 Terminator
     misc_feature    336..388
                     /label=attL
     misc_feature    389..437
                     /label=homologous sequence B
     RBS             438..451
                     /label=BBa_B0031
     misc_feature    438..2083
                     /label=BBa_1433006
     CDS             452..1954
                     /label=BBa_K907000 Bxb1 DNA integrase，gp35
     stem_loop       1955..2083
                     /label=BBa_B0015 Terminator
ORIGIN
        1 tttacggcta gctcagtcct aggtacaatg ctagcgacca agatcgagac gctgcgcctg
       61 gccatccgct acatctcggc cggcttgtcg acgacggcgg tctcagtggt gtacggtaca
      121 aaccctctag ggcggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat
      181 aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc tctagggcgg
      241 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
      301 gtctttcgac tgagcctttc gttttatttg atgccgcccg gatgatcctg acgacggaga
      361 ccgcggtggt tgaccagaca aaccacgatt cttatgtgcc ctcctcccac tcctctcccc
      421 agacttcatc gccttcttca cacaggaaac catgagagcc ctggtagtca tccgcctgtc
      481 ccgcgtcacc gatgctacga cttcaccgga gcgtcagctg gagtcttgcc agcagctctg
      541 cgcccagcgc ggctgggacg tcgtcggggt agcggaggat ctggacgtct ccggggcggt
      601 cgatccgttc gaccggaagc gcagaccgaa cctggcccgg tggctagcgt tcgaggagca
      661 accgttcgac gtgatcgtgg cgtaccgggt agaccggttg acccgatcga tccggcatct
      721 gcaacagctg gtccactggg ccgaggacca caagaagctg gtcgtctccg cgaccgaagc
      781 gcacttcgat acgacgacgc cgtttgcggc ggtcgtcatc gcgcttatgg gaacggtggc
      841 gcagatggaa ttagaagcga tcaaagagcg gaaccgttcg gctgcgcatt tcaatatccg
      901 cgccgggaaa taccgaggat ccctgccgcc gtggggatac ctgcctacgc gcgtggacgg
      961 ggagtggcgg ctggtgccgg accctgtgca gcgagagcgc atcctcgagg tgtatcaccg
     1021 cgtcgtcgac aaccacgagc cgctgcacct ggtggcccac gacctgaacc ggcgtggtgt
     1081 cctgtcgccg aaggactact tcgcgcagct gcaaggccgc gagccgcagg gccgggagtg
     1141 gtcggctacc gcgctgaagc gatcgatgat ctccgaggcg atgctcgggt acgcgactct
     1201 gaacggtaag accgtccgag acgacgacgg agccccgctg gtgcgggctg agccgatcct
     1261 gacccgtgag cagctggagg cgctgcgcgc cgagctcgtg aagacctccc gggcgaagcc
     1321 cgcggtgtct accccgtcgc tgctgctgcg ggtgttgttc tgcgcggtgt gcggggagcc
     1381 cgcgtacaag ttcgccgggg gaggacgtaa gcacccgcgc taccgctgcc gctcgatggg
     1441 gttcccgaag cactgcggga acggcacggt ggcgatggcc gagtgggacg cgttctgcga
     1501 ggagcaggtg ctggatctgc tcggggacgc ggagcgtctg gagaaagtct gggtagccgg
     1561 ctcggactcc gcggtcgaac tcgcggaggt gaacgcggag ctggtggacc tgacgtcgct
     1621 gatcggctcc ccggcctacc gggccggctc tccgcagcga gaagcactgg atgcccgtat
     1681 tgcggcgctg gccgcgcggc aagaggagct ggagggccta gaggctcgcc cgtctggctg
     1741 ggagtggcgc gagaccgggc agcggttcgg ggactggtgg cgggagcagg acaccgcggc
     1801 aaagaacacc tggcttcggt cgatgaacgt tcggctgacg ttcgacgtcc gcggcgggct
     1861 gactcgcacg atcgacttcg gggatctgca agagtacgag cagcatctca ggctcggcag
     1921 cgtggtcgaa cggctacaca ccgggatgtc gtagccaggc atcaaataaa acgaaaggct
     1981 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
     2041 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
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