LOCUS BBa_K1433022 5982 bp DNA linear 25-Nov-2024 DEFINITION rT-rKan-rRBS-attB-P-attP-RBS-lambda red-RBS-Chl-T-P-RBS-gp47-tag-T ACCESSION BBa_K1433022 VERSION BBa_K1433022.1 FEATURES Location/Qualifiers stem_loop 1..129 /label=BBa_B0015 Terminator CDS 130..945 /label=Kan resistance CDS RBS 946..957 /label=BBa_B0034 RBS misc_feature 958..1007 /label=attB promoter 1008..1042 /label=BBa_J23110 promoter misc_feature 1043..1095 /label=attP RBS 1096..1114 /label=RBS CDS 1115..1431 /label=GAM-CDS CDS 1437..2222 /label=Beta-CDS CDS 2219..2899 /label=EXO-CDS RBS 2900..2912 /label=BBa_B0034 RBS CDS 2913..3673 /label=Chl resistance CDS stem_loop 3674..3802 /label=BBa_B0015 Terminator promoter 3803..5012 /label=BBa_I0500 Inducible pBad/araC promoter RBS 5013..5037 /label=RBS CDS 5038..5814 /label=BBa_K1433004 Bxb1 excisionase gp447 misc_feature 5815..5851 /label=AAK tag stem_loop 5852..5982 /label=BBa_B0015 Terminator ORIGIN 1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca 61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt 121 gatgcctggt tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg 181 attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag 241 gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc 301 aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg 361 agtgacgact gaatccggtg agaatggcaa aagcttatgc atttctttcc agacttgttc 421 aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat 481 tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac 541 aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga 601 atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa 661 ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt 721 cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg 781 tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga 841 ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt 901 taatcgcggc ctggagcaag acgtttcccg ttgaatatgg ctcattttct cctcttttcg 961 gccggcttgt cgacgacggc ggtctccgtc gtcaggatca tccgggcttt acggctagct 1021 cagtcctagg tacaatgcta gcgggtttgt accgtacacc actgagaccg cggtggttga 1081 ccagacaaac cacgatctaa ggaggttata aaaaatggat attaatactg aaactgagat 1141 caagcaaaag cattcactaa ccccctttcc tgttttccta atcagcccgg catttcgcgg 1201 gcgatatttt cacagctatt tcaggagttc agccatgaac gcttattaca ttcaggatcg 1261 tcttgaggct cagagctggg cgcgtcacta ccagcagctc gcccgtgaag agaaagaggc 1321 agaactggca gacgacatgg aaaaaggcct gccccagcac ctgtttgaat cgctatgcat 1381 cgatcatttg caacgccacg gggccagcaa aaaatccatt acccgtgcgt ttgatgacga 1441 tgttgagttt caggagcgca tggcagaaca catccggtac atggttgaaa ccattgctca 1501 ccaccaggtt gatattgatt cagaggtata aaacgaatga gtactgcact cgcaacgctg 1561 gctgggaagc tggctgaacg tgtcggcatg gattctgtcg acccacagga actgatcacc 1621 actcttcgcc agacggcatt taaaggtgat gccagcgatg cgcagttcat cgcattactg 1681 atcgttgcca accagtacgg ccttaatccg tggacgaaag aaatttacgc ctttcctgat 1741 aagcagaatg gcatcgttcc ggtggtgggc gttgatggct ggtcccgcat catcaatgaa 1801 aaccagcagt ttgatggcat ggactttgag caggacaatg aatcctgtac atgccggatt 1861 taccgcaagg accgtaatca tccgatctgc gttaccgaat ggatggatga atgccgccgc 1921 gaaccattca aaactcgcga aggcagagaa atcacggggc cgtggcagtc gcatcccaaa 1981 cggatgttac gtcataaagc catgattcag tgtgcccgtc tggccttcgg atttgctggt 2041 atctatgaca aggatgaagc cgagcgcatt gtcgaaaata ctgcatacac ggcagaacgt 2101 cagccggaac gcgacatcac tccggttaac gatgaaacca tgcaggagat taacactctg 2161 ctgatcgccc tggataaaac atgggatgac gacttattgc cgctctgttc ccagatattt 2221 cgccgcgaca ttcgtgcatc gtcagaactg acacaggccg aagcagtaaa agctcttgga 2281 ttcctgaaac agaaagccgc agagcagaag gtggcagcat gacaccggac attatcctgc 2341 aacgtaccgg gatcgatgtg agagctgtcg aacaggggga tgatgcgtgg cacaaattac 2401 ggctcggcgt catcaccgct tcagaagttc acaacgtgat agcaaaaccc cgctccggaa 2461 agaagtggcc tgacatgaaa atgtcctact tccacaccct gcttgctgag gtttgcaccg 2521 gtgtggctcc ggaagttaac gctaaagcac tggcctgggg aaaacagtac gagaacgacg 2581 ccagaaccct gtttgagttc acttccggcg tgaatgttac tgaatccccg atcatctatc 2641 gcgacgaaag tatgcgtacc gcctgctctc ccgatggttt atgcagtgac ggcaacggcc 2701 ttgaactgaa atgcccgttt acctcccggg atttcatgaa gttccggctc ggtggtttcg 2761 aggccataaa gtcagcttac atggcccagg tgcagtacag catgtgggtg acgcgaaaaa 2821 atgcctggta ctttgccaac tatgacccgc gtatgaagcg tgaaggcctg cattatgtcg 2881 tgattgagcg ggatgaaaag tacatggcga gttttgacga gatcgtgccg gagttcatcg 2941 aaaaaatgga cgaggcactg gctgaaattg gttttgtatt tggggagcaa tggcgatgaa 3001 aagaggagaa aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc 3061 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg 3121 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc 3181 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattt cgtatggcaa 3241 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg 3301 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc 3361 tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag 3421 ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg 3481 atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt 3541 atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg 3601 atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg 3661 gcggggcgta attccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt 3721 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg 3781 ggtgggcctt tctgcgttta tattatgaca acttgacggc tacatcattc actttttctt 3841 cacaaccggc acggaactcg ctcgggctgg ccccggtgca ttttttaaat acccgcgaga 3901 aatagagttg atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc atccgggtgg 3961 tgctcaaaag cagcttcgcc tggctgatac gttggtcctc gcgccagctt aagacgctaa 4021 tccctaactg ctggcggaaa agatgtgaca gacgcgacgg cgacaagcaa acatgctgtg 4081 cgacgctggc gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct 4141 cgcgtacccg attatccatc ggtggatgga gcgactcgtt aatcgcttcc atgcgccgca 4201 gtaacaattg ctcaagcaga tttatcgcca gcagctccga atagcgccct tccccttgcc 4261 cggcgttaat gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct tcatccgggc 4321 gaaagaaccc cgtattggca aatattgacg gccagttaag ccattcatgc cagtaggcgc 4381 gcggacgaaa gtaaacccac tggtgatacc attcgcgagc ctccggatga cgaccgtagt 4441 gatgaatctc tcctggcggg aacagcaaaa tatcacccgg tcggcaaaca aattctcgtc 4501 cctgattttt caccaccccc tgaccgcgaa tggtgagatt gagaatataa cctttcattc 4561 ccagcggtcg gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc gttaaacccg 4621 ccaccagatg ggcattaaac gagtatcccg gcagcagggg atcattttgc gcttcagcca 4681 tacttttcat actcccgcca ttcagagaag aaaccaattg tccatattgc atcagacatt 4741 gccgtcactg cgtcttttac tggctcttct cgctaaccaa accggtaacc ccgcttatta 4801 aaagcattct gtaacaaagc gggaccaaag ccatgacaaa aacgcgtaac aaaagtgtct 4861 ataatcacgg cagaaaagtc cacattgatt atttgcacgg cgtcacactt tgctatgcca 4921 tagcattttt atccataaga ttagcggatc ctacctgacg ctttttatcg caactctcta 4981 ctgtttctcc atacccgttt ttttgggcta gcaccttaac aaactacgga ggtcaacatg 5041 gccggcactc agcgtatcgt ctttctaccc gatactcagt tgcctttcga ggcgcgcaaa 5101 gagatgcaag cggtcatccg cttcatcggg gatgtccagc cgtacggcgt ggtacatatc 5161 ggtgacgtcc tagacctgcc gcagccctcg cgctggaacc ggggaaccaa gggcgagttc 5221 gagggctcgg tgtaccgcga cgcggactac gccaagaaga acctgatgga gccgctgcgc 5281 aaggtctacg acggctggat cgggatgcac gagggcaacc acgatctgcg agcccgcgag 5341 tacctggcca agaacgcacc ggccctggag ggtacgcacg ctttcgacat cgacgtgctg 5401 ctcgacttcg acgggttcgg tgtggagctg ctgcctgact tctacgacat cgctccgggc 5461 tggatctcca ctcacgggca catgggcaag atgacgctat cccagatcgc cggatcgaca 5521 gcgctcaacg gtgccaagaa gttcggcaag tccgtggtct gcggccacac gcaccggcag 5581 gctgtcgtct cgcactcgtt cgggtacggc ggctcggtgc gcaagaccgt caccggcatg 5641 gaagtcgggc acctgatgga catgaagaag gccaactatc taaagggcgg agccgggaac 5701 tggcagatgg gcttcgggat gctcacggtc gacggcaagc atgtcaaggc tgagatcgtc 5761 ccgatcctgg gaggcaagtt caccgttgac ggccaggtct gggaagtcac cggtgcagca 5821 aacgacgaaa actacgctgc tgctaaataa taaccaggca tcaaataaaa cgaaaggctc 5881 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga 5941 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta //