LOCUS       BBa_K1435002            1484 bp    DNA     linear       22-Nov-2024
DEFINITION  N-terminal GFP w/ an N-terminal Intein
ACCESSION   BBa_K1435002
VERSION     BBa_K1435002.1  
FEATURES             Location/Qualifiers
     CDS             1..18
                     /label=His6 Tag
     CDS             19..30
                     /label=Linker
     CDS             31..549
                     /label=N-terminal GFP
     CDS             550..850
                     /label=N-terminal Intein
     misc_feature    851..1358
                     /label=Yeast Terminator
     misc_feature    1359..1484
                     /label=E.coli Double Terminator
ORIGIN
        1 catcatcacc atcaccacgg tggttctggt gctgaggccg ccgcaaaaga agcagcagct
       61 aaggaagctg cggcgaaggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
      121 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
      181 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
      241 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
      301 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
      361 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
      421 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
      481 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
      541 gacaagcagt gcattagcgg cgatagcctg attagcctgg cgagcaccgg caaacgcgtg
      601 agcattaaag atctgctgga tgaaaaagat tttgaaattt gggcgattaa cgaacagacc
      661 atgaaactgg aaagcgcgaa agtgagccgc gtgttttgca ccggcaaaaa actggtgtat
      721 attctgaaaa cccgcctggg ccgcaccatt aaagcgaccg cgaaccatcg ctttctgacc
      781 attgatggct ggaaacgcct ggatgaactg agcctgaaag aacatattgc gctgccgcgc
      841 aaactggaag gagattgata agacttttct agttgcatat cttttatatt taaatcttat
      901 ctattagtta attttttgta atttatcctt atatatagtc tggttattct aaaatatcat
      961 ttcagtatct aaaaattccc ctcttttttc agttatatct taacaggcga cagtccaaat
     1021 gttgatttat cccagtccga ttcatcaggg ttgtgaagca ttttgtcaat ggtcgaaatc
     1081 acatcagtaa tagtgcctct tacttgcctc atagaatttc tttctcttaa cgtcaccgtt
     1141 tgtcttttat agtttcgaaa tctatggtga taccaaatgg tgttcccaat tcatcgttac
     1201 gggcgtattt tttaccaatt gaagtattgg aatcgtcaat tttaaagtat atctctcttt
     1261 tacgtaaagc ctgcgagatc ctcttaagta tagcggggaa gccatcgtta ttcgatattg
     1321 tcgtaacaaa tactttgagc ggcgctatct gtaatgcagg catcaaataa aacgaaaggc
     1381 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
     1441 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//