BBa_K1435002
1
BBa_K1435002
N-terminal GFP w/ an N-terminal Intein
2014-10-16T11:00:00Z
2015-05-08T01:10:25Z
This part was created using de novo DNA synthesis. The intein sequence was obtained via InBase, a peer-reviewed database of inteins. The intein sequence was isolated from Cyanobacterium synechocystis.
This part is a N-terminal GFP with an intein fused to it. In the presence of the C-terminal intein conjugated to the C-terminal GFP, the intein will auto catalytically splice itself out and ligate its exteins together, forming the functional GFP.
false
false
_1813_
0
20493
9
It's complicated
false
N/A
false
Matthew Chan
annotation2422876
1
Yeast Terminator
range2422876
1
851
1358
annotation2422875
1
N-terminal Intein
range2422875
1
550
850
annotation2422872
1
His6 Tag
range2422872
1
1
18
annotation2422873
1
Linker
range2422873
1
19
30
annotation2422874
1
N-terminal GFP
range2422874
1
31
549
annotation2422877
1
E.coli Double Terminator
range2422877
1
1359
1484
BBa_K1435002_sequence
1
catcatcaccatcaccacggtggttctggtgctgaggccgccgcaaaagaagcagcagctaaggaagctgcggcgaaggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagtgcattagcggcgatagcctgattagcctggcgagcaccggcaaacgcgtgagcattaaagatctgctggatgaaaaagattttgaaatttgggcgattaacgaacagaccatgaaactggaaagcgcgaaagtgagccgcgtgttttgcaccggcaaaaaactggtgtatattctgaaaacccgcctgggccgcaccattaaagcgaccgcgaaccatcgctttctgaccattgatggctggaaacgcctggatgaactgagcctgaaagaacatattgcgctgccgcgcaaactggaaggagattgataagacttttctagttgcatatcttttatatttaaatcttatctattagttaattttttgtaatttatccttatatatagtctggttattctaaaatatcatttcagtatctaaaaattcccctcttttttcagttatatcttaacaggcgacagtccaaatgttgatttatcccagtccgattcatcagggttgtgaagcattttgtcaatggtcgaaatcacatcagtaatagtgcctcttacttgcctcatagaatttctttctcttaacgtcaccgtttgtcttttatagtttcgaaatctatggtgataccaaatggtgttcccaattcatcgttacgggcgtattttttaccaattgaagtattggaatcgtcaattttaaagtatatctctcttttacgtaaagcctgcgagatcctcttaagtatagcggggaagccatcgttattcgatattgtcgtaacaaatactttgagcggcgctatctgtaatgcaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z