LOCUS       BBa_K1439001            1427 bp    DNA     linear       16-Feb-2025
DEFINITION  This part contains a reporter gene BBa_J04450, combined with 
            OriTRP4. Used to test plasmid mobility.
ACCESSION   BBa_K1439001
VERSION     BBa_K1439001.1  
FEATURES             Location/Qualifiers
     oriT            1..350
                     /label=BBa_K1439000
     CDS             359..446
                     /label=end of LacI coding region (inactive)
     misc_feature    359..558
                     /label=BBa_R0010
     misc_feature    447..484
                     /label=CAP binding site
     promoter        495..500
                     /label=-35
     promoter        519..524
                     /label=-10
     misc_feature    524..558
                     /label=LacI binding site
     misc_feature    531..531
                     /label=start
     promoter        359..558
                     /label=BBa_R0010
     misc_feature    571..574
                     /label=conserved
     RBS             567..578
                     /label=BBa_B0034
     CDS             585..1259
                     /label=mrfp1
     misc_feature    1266..1290
                     /label=Help:Barcodes
     CDS             585..1265
                     /label=BBa_E1010
     stem_loop       1310..1353
                     /label=stem_loop
     misc_feature    1299..1378
                     /label=BBa_B0010
     terminator      1299..1378
                     /label=BBa_B0010
     stem_loop       1394..1413
                     /label=T7 TE
     misc_feature    1420..1420
                     /label=stop
     polyA_site      1414..1427
                     /label=polya
     misc_feature    1387..1427
                     /label=BBa_B0012
     terminator      1387..1427
                     /label=BBa_B0012
     misc_feature    359..1427
                     /label=BBa_J04450
ORIGIN
        1 gcttgccctc atctgttacg ccggcggtag ccggccagcc tcgcagagca ggattcccgt
       61 tgagcaccgc caggtgcgaa taagggacag tgaagaagga acacccgctc gcgggtgggc
      121 ctacttcacc tatcctgccc ggctgacgcc gttggataca ccaaggaaag tctacacgaa
      181 ccctttggca aaatcctgta tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta
      241 taatgacccc gaagcagggt tatgcagcgg aaaagcgctg cttccctgct gttttgtgga
      301 atatctaccg actggaaaca ggcaaatgca ggaaattact gaactgaggg tactagagca
      361 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
      421 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
      481 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
      541 ggataacaat ttcacacata ctagagaaag aggagaaata ctagatggct tcctccgaag
      601 acgttatcaa agagttcatg cgtttcaaag ttcgtatgga aggttccgtt aacggtcacg
      661 agttcgaaat cgaaggtgaa ggtgaaggtc gtccgtacga aggtacccag accgctaaac
      721 tgaaagttac caaaggtggt ccgctgccgt tcgcttggga catcctgtcc ccgcagttcc
      781 agtacggttc caaagcttac gttaaacacc cggctgacat cccggactac ctgaaactgt
      841 ccttcccgga aggtttcaaa tgggaacgtg ttatgaactt cgaagacggt ggtgttgtta
      901 ccgttaccca ggactcctcc ctgcaagacg gtgagttcat ctacaaagtt aaactgcgtg
      961 gtaccaactt cccgtccgac ggtccggtta tgcagaaaaa aaccatgggt tgggaagctt
     1021 ccaccgaacg tatgtacccg gaagacggtg ctctgaaagg tgaaatcaaa atgcgtctga
     1081 aactgaaaga cggtggtcac tacgacgctg aagttaaaac cacctacatg gctaaaaaac
     1141 cggttcagct gccgggtgct tacaaaaccg acatcaaact ggacatcacc tcccacaacg
     1201 aagactacac catcgttgaa cagtacgaac gtgctgaagg tcgtcactcc accggtgctt
     1261 aataacgctg atagtgctag tgtagatcgc tactagagcc aggcatcaaa taaaacgaaa
     1321 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     1381 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//