##gff-version 3
##sequence-region BBa_K1439001 1 1427
BBa_K1439001	.	oriT	1	350	.	+	0	ID=BBa_K1439000;Name=BBa_K1439000
BBa_K1439001	.	engineered_region	359	1427	.	+	0	ID=BBa_J04450;Name=BBa_J04450
BBa_K1439001	.	promoter	359	558	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04450
BBa_K1439001	.	non_covalent_binding_site	447	484	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1439001	.	promoter	519	524	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1439001	.	engineered_region	359	558	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1439001	.	CDS	359	446	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1439001	.	non_covalent_binding_site	524	558	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1439001	.	promoter	495	500	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1439001	.	start_codon	531	531	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1439001	.	terminator	1299	1378	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04450
BBa_K1439001	.	engineered_region	1299	1378	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1439001	.	stem_loop	1310	1353	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1439001	.	CDS	585	1265	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04450
BBa_K1439001	.	CDS	585	1259	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1439001	.	engineered_tag	1266	1290	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1439001	.	ribosome_entry_site	567	578	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04450
BBa_K1439001	.	conserved_region	571	574	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1439001	.	terminator	1387	1427	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04450
BBa_K1439001	.	engineered_region	1387	1427	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1439001	.	stem_loop	1394	1413	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1439001	.	polyA_site	1414	1427	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1439001	.	stop_codon	1420	1420	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
>BBa_K1439001
gcttgccctcatctgttacgccggcggtagccggccagcctcgcagagcaggattcccgttgagcaccgccaggtgcga
ataagggacagtgaagaaggaacacccgctcgcgggtgggcctacttcacctatcctgcccggctgacgccgttggata
caccaaggaaagtctacacgaaccctttggcaaaatcctgtatatcgtgcgaaaaaggatggatataccgaaaaaatcg
ctataatgaccccgaagcagggttatgcagcggaaaagcgctgcttccctgctgttttgtggaatatctaccgactgga
aacaggcaaatgcaggaaattactgaactgagggtactagagcaatacgcaaaccgcctctccccgcgcgttggccgat
tcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctc
actcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttc
acacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagtt
cgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccaga
ccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttc
caaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgt
gttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaag
ttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccga
acgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgac
gctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggaca
tcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaata
acgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcct
ttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgt
ttata