LOCUS BBa_K1439003 3379 bp DNA linear 18-May-2024 DEFINITION [OriTR-RFP]+[lysis] ACCESSION BBa_K1439003 VERSION BBa_K1439003.1 FEATURES Location/Qualifiers oriT 1..371 /label=BBa_J01003 CDS 380..467 /label=end of LacI coding region (inactive) misc_feature 380..579 /label=BBa_R0010 misc_feature 468..505 /label=CAP binding site promoter 516..521 /label=-35 promoter 540..545 /label=-10 misc_feature 545..579 /label=LacI binding site misc_feature 552..552 /label=start promoter 380..579 /label=BBa_R0010 misc_feature 592..595 /label=conserved RBS 588..599 /label=BBa_B0034 CDS 606..1280 /label=mrfp1 misc_feature 1287..1311 /label=Help:Barcodes CDS 606..1286 /label=BBa_E1010 stem_loop 1331..1374 /label=stem_loop misc_feature 1320..1399 /label=BBa_B0010 terminator 1320..1399 /label=BBa_B0010 stem_loop 1415..1434 /label=T7 TE misc_feature 1441..1441 /label=stop polyA_site 1435..1448 /label=polya misc_feature 1408..1448 /label=BBa_B0012 terminator 1408..1448 /label=BBa_B0012 misc_feature 380..1448 /label=BBa_J04450 misc_feature 1..1448 /label=BBa_K1439002 promoter 1457..1587 /label=promoter misc_feature 1496..1513 /label=AraI1 misc_feature 1517..1534 /label=AraI2 promoter 1457..1586 /label=BBa_K206000 CDS 1611..2267 /label=T4 holin CDS 1595..2270 /label=BBa_K112805 CDS 2295..2789 /label=T4 Endolysin CDS 2279..2792 /label=BBa_K112806 stem_loop 2812..2855 /label=stem_loop misc_feature 2801..2880 /label=BBa_B0010 terminator 2801..2880 /label=BBa_B0010 stem_loop 2896..2915 /label=T7 TE misc_feature 2922..2922 /label=stop polyA_site 2916..2929 /label=polya misc_feature 2889..2929 /label=BBa_B0012 terminator 2889..2929 /label=BBa_B0012 promoter 2938..2972 /label=BBa_J23116 CDS 2995..3288 /label=T4 antiholin CDS 2981..3291 /label=BBa_K112807 stem_loop 3311..3354 /label=stem_loop misc_feature 3300..3379 /label=BBa_B0010 terminator 3300..3379 /label=BBa_B0010 misc_feature 1595..3379 /label=BBa_K112808 misc_feature 1457..3379 /label=BBa_K1439009 ORIGIN 1 gccgcctttt cctcaatcgc tcttcgttcg tctggaaggc agtacacctt gataggtggg 61 ctgcccttcc tggttggctt ggtttcatca gccatccgct tgccctcatc tgttacgccg 121 gcggtagccg gccagcctcg cagagcagga ttcccgttga gcaccgccag gtgcgaataa 181 gggacagtga agaaggaaca cccgctcgcg ggtgggccta cttcacctat cctgcccggc 241 tgacgccgtt ggatacacca aggaaagtct acacgaaccc tttggcaaaa tcctgtatat 301 cgtgcgaaaa aggatggata taccgaaaaa atcgctataa tgaccccgaa gcagggttat 361 gcagcggaaa atactagagc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat 421 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt 481 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt 541 atgttgtgtg gaattgtgag cggataacaa tttcacacat actagagaaa gaggagaaat 601 actagatggc ttcctccgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg 661 aaggttccgt taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg 721 aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg 781 acatcctgtc cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca 841 tcccggacta cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact 901 tcgaagacgg tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca 961 tctacaaagt taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa 1021 aaaccatggg ttgggaagct tccaccgaac gtatgtaccc ggaagacggt gctctgaaag 1081 gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa 1141 ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac 1201 tggacatcac ctcccacaac gaagactaca ccatcgttga acagtacgaa cgtgctgaag 1261 gtcgtcactc caccggtgct taataacgct gatagtgcta gtgtagatcg ctactagagc 1321 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg 1381 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg 1441 cgtttatata ctagagacat tgattatttg cacggcgtca cactttgcta tgccatagca 1501 agatagtcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 1561 tctccatacc gtttttttgg gctagctact agagcttaaa aggagggtct atggcagcac 1621 ctagaatatc attttcgccc tctgatattc tatttggtgt tctcgatcgc ttgttcaaag 1681 ataacgctac cgggaaggtt cttgcttccc gggtagctgt cgtaattctt ttgtttataa 1741 tggcgattgt ttggtatagg ggagatagtt tctttgagta ctataagcaa tcaaagtatg 1801 aaacatacag tgaaattatt gaaaaggaaa gaactgcacg ctttgaatct gtcgccctgg 1861 aacaactcca gatagttcat atatcatctg aggcagactt tagtgcggtg tattctttcc 1921 gccctaaaaa cttaaactat tttgttgata ttatagcata cgaaggaaaa ttaccttcaa 1981 caataagtga aaaatcactt ggaggatatc ctgttgataa aactatggat gaatatacag 2041 ttcatttaaa tggacgtcat tattattcca actcaaaatt tgctttttta ccaactaaaa 2101 agcctactcc cgaaataaac tacatgtaca gttgtccata ttttaatttg gataatatct 2161 atgctggaac gataaccatg tactggtata gaaatgatca tataagtaat gaccgccttg 2221 aatcaatatg tgctcaggcg gccagaatat taggaagggc taaataatta tactagagat 2281 acttaggagg tattatgaat atatttgaaa tgttacgtat agatgaaggt cttagactta 2341 aaatctataa agacacagaa ggctattaca ctattggcat cggtcatttg cttacaaaaa 2401 gtccatcact taatgctgct aaatctgaat tagataaagc tattgggcgt aattgcaatg 2461 gtgtaattac aaaagatgag gctgaaaaac tctttaatca ggatgttgat gctgctgttc 2521 gcggaatcct gagaaatgct aaattaaaac cggtttatga ttctcttgat gcggttcgtc 2581 gctgtgcatt gattaatatg gttttccaaa tgggagaaac cggtgtggca ggatttacta 2641 actctttacg tatgcttcaa caaaaacgct gggatgaagc agcagttaac ttagctaaaa 2701 gtagatggta taatcaaaca cctaatcgcg caaaacgagt cattacaacg tttagaactg 2761 gcacttggga cgcgtataaa aatctataaa gctactagag ccaggcatca aataaaacga 2821 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 2881 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagagttg 2941 acagctagct cagtcctagg gactatgcta gctactagag gataggaggc ctttatggcc 3001 ttaaaagcaa cagcactttt tgccatgcta ggattgtcat ttgttttatc tccatcgatt 3061 gaagcgaatg tcgatcctca ttttgataaa tttatggaat ctggtattag gcacgtttat 3121 atgctttttg aaaataaaag cgtagaatcg tctgaacaat tctatagttt tatgagaacg 3181 acctataaaa atgacccgtg ctcttctgat tttgaatgta tagagcgagg cgcggagatg 3241 gcacaatcat acgctagaat tatgaacatt aaattggaga ctgaatgaaa ttactagagc 3301 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg 3361 tttgtcggtg aacgctctc //