BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916612
1
BBa_B0012
component1916610
1
BBa_B0010
annotation1916610
1
BBa_B0010
range1916610
1
1
80
annotation1916612
1
BBa_B0012
range1916612
1
89
129
BBa_K1439004
1
BBa_K1439004
TAT-H4
2014-10-04T11:00:00Z
2015-05-08T01:10:26Z
Histone H4 comes from the Wheat histone H4 gene, and TAT-PTD comes from HIV-1's TAT protein gene.
Histone H4 can efficiently mediate gene transfer, it rich in positively charged alkaline amino acids and has the ability to combine with DNA. TAT-PTD contains an 11-amino acid peptide, and these amino acids YGRKKRRQRRR are highly basic, which is crucial for penetrating the plasma membrane. We use linker to link H4 histone and TAT, thus it can seize DNA and take it into cells. Now, it becomes our transfer device.
false
false
_1817_
0
21804
9
It's complicated
false
We found the sequence from NCBI, and synthetized primer.
false
Zhaoliang Chen
annotation2394213
1
misc
range2394213
1
1
393
BBa_K1439008
1
BBa_K1439008
TAT-H4-B0015
2014-10-04T11:00:00Z
2015-05-08T01:10:26Z
TAT-H4 comes from the BBa_K1439004 and the Terminator comes from BBa_B0015
H4 histone can efficiently mediate gene transfer, it rich in positively charged alkaline amino acids and has the ability to combine with DNA. TAT-PTD contains an 11-amino acid peptide, and these amino acids YGRKKRRQRRR are highly basic, which is crucial for penetrating the plasma membrane. We use linker to link H4 histone and TAT, thus it can seize DNA and take it into cells. Now, it becomes our transfer device.
false
false
_1817_
0
21804
9
It's complicated
false
We got them form iGEM.
false
Zhaoliang Chen
component2394294
1
BBa_K1439004
component2394301
1
BBa_B0015
annotation2394294
1
BBa_K1439004
range2394294
1
1
393
annotation2394301
1
BBa_B0015
range2394301
1
402
530
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation1690
1
polya
range1690
1
28
41
annotation1687
1
stop
range1687
1
34
34
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K1439004_sequence
1
atgtatggtcgtaaaaaacgtcgtcagcgtcgtcgcggtggaggaggctctggtggaggcggtagcggaggcggagggtcgatgtccgggcgcggcaagggaggcaagggcctaggcaagggcggcgccaagcgccaccggaaggtcctccgcgataacatccagggcatcaccaagccggcgatccggcggctggcgcggcggggcggcgtgaagcgcatctcggggctcatctacgaggagacccgcggcgtgctcaagatcttcctcgagaacgtcatccgcgatgccgtcacctacaccgagcacgcccgccgcaagaccgtcaccgccatggacgtcgtctacgcgctcaagcgccagggccgcaccctctacggcttcggcggctaa
BBa_K1439008_sequence
1
atgtatggtcgtaaaaaacgtcgtcagcgtcgtcgcggtggaggaggctctggtggaggcggtagcggaggcggagggtcgatgtccgggcgcggcaagggaggcaagggcctaggcaagggcggcgccaagcgccaccggaaggtcctccgcgataacatccagggcatcaccaagccggcgatccggcggctggcgcggcggggcggcgtgaagcgcatctcggggctcatctacgaggagacccgcggcgtgctcaagatcttcctcgagaacgtcatccgcgatgccgtcacctacaccgagcacgcccgccgcaagaccgtcaccgccatggacgtcgtctacgcgctcaagcgccagggccgcaccctctacggcttcggcggctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z