LOCUS       BBa_K1439010            2738 bp    DNA     linear       22-Nov-2024
DEFINITION  Lysis device induced by aTc
ACCESSION   BBa_K1439010
VERSION     BBa_K1439010.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23106
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             65..681
                     /label=tetR
     misc_feature    682..715
                     /label=SsrA
     misc_feature    722..746
                     /label=Help:Barcodes
     CDS             62..721
                     /label=BBa_C0040
     stem_loop       766..809
                     /label=stem_loop
     misc_feature    755..834
                     /label=BBa_B0010
     terminator      755..834
                     /label=BBa_B0010
     stem_loop       850..869
                     /label=T7 TE
     misc_feature    876..876
                     /label=stop
     polyA_site      870..883
                     /label=polya
     misc_feature    843..883
                     /label=BBa_B0012
     terminator      843..883
                     /label=BBa_B0012
     misc_feature    44..883
                     /label=BBa_P0440
     misc_feature    892..910
                     /label=TetR 1
     misc_feature    892..945
                     /label=BBa_R0040
     promoter        911..916
                     /label=-35
     misc_feature    917..935
                     /label=TetR 2
     promoter        934..939
                     /label=-10
     promoter        892..945
                     /label=BBa_R0040
     CDS             970..1626
                     /label=T4 holin
     CDS             954..1629
                     /label=BBa_K112805
     CDS             1654..2148
                     /label=T4 Endolysin
     CDS             1638..2151
                     /label=BBa_K112806
     stem_loop       2171..2214
                     /label=stem_loop
     misc_feature    2160..2239
                     /label=BBa_B0010
     terminator      2160..2239
                     /label=BBa_B0010
     stem_loop       2255..2274
                     /label=T7 TE
     misc_feature    2281..2281
                     /label=stop
     polyA_site      2275..2288
                     /label=polya
     misc_feature    2248..2288
                     /label=BBa_B0012
     terminator      2248..2288
                     /label=BBa_B0012
     promoter        2297..2331
                     /label=BBa_J23116
     CDS             2354..2647
                     /label=T4 antiholin
     CDS             2340..2650
                     /label=BBa_K112807
     stem_loop       2670..2713
                     /label=stem_loop
     misc_feature    2659..2738
                     /label=BBa_B0010
     terminator      2659..2738
                     /label=BBa_B0010
     misc_feature    954..2738
                     /label=BBa_K112808
ORIGIN
        1 tttacggcta gctcagtcct aggtatagtg ctagctacta gagaaagagg agaaatacta
       61 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt
      121 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac
      181 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt
      241 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg
      301 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca
      361 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt
      421 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt
      481 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac
      541 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca
      601 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca
      661 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata
      721 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct
      781 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
      841 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gtccctatca
      901 gtgatagaga ttgacatccc tatcagtgat agagatactg agcactacta gagcttaaaa
      961 ggagggtcta tggcagcacc tagaatatca ttttcgccct ctgatattct atttggtgtt
     1021 ctcgatcgct tgttcaaaga taacgctacc gggaaggttc ttgcttcccg ggtagctgtc
     1081 gtaattcttt tgtttataat ggcgattgtt tggtataggg gagatagttt ctttgagtac
     1141 tataagcaat caaagtatga aacatacagt gaaattattg aaaaggaaag aactgcacgc
     1201 tttgaatctg tcgccctgga acaactccag atagttcata tatcatctga ggcagacttt
     1261 agtgcggtgt attctttccg ccctaaaaac ttaaactatt ttgttgatat tatagcatac
     1321 gaaggaaaat taccttcaac aataagtgaa aaatcacttg gaggatatcc tgttgataaa
     1381 actatggatg aatatacagt tcatttaaat ggacgtcatt attattccaa ctcaaaattt
     1441 gcttttttac caactaaaaa gcctactccc gaaataaact acatgtacag ttgtccatat
     1501 tttaatttgg ataatatcta tgctggaacg ataaccatgt actggtatag aaatgatcat
     1561 ataagtaatg accgccttga atcaatatgt gctcaggcgg ccagaatatt aggaagggct
     1621 aaataattat actagagata cttaggaggt attatgaata tatttgaaat gttacgtata
     1681 gatgaaggtc ttagacttaa aatctataaa gacacagaag gctattacac tattggcatc
     1741 ggtcatttgc ttacaaaaag tccatcactt aatgctgcta aatctgaatt agataaagct
     1801 attgggcgta attgcaatgg tgtaattaca aaagatgagg ctgaaaaact ctttaatcag
     1861 gatgttgatg ctgctgttcg cggaatcctg agaaatgcta aattaaaacc ggtttatgat
     1921 tctcttgatg cggttcgtcg ctgtgcattg attaatatgg ttttccaaat gggagaaacc
     1981 ggtgtggcag gatttactaa ctctttacgt atgcttcaac aaaaacgctg ggatgaagca
     2041 gcagttaact tagctaaaag tagatggtat aatcaaacac ctaatcgcgc aaaacgagtc
     2101 attacaacgt ttagaactgg cacttgggac gcgtataaaa atctataaag ctactagagc
     2161 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     2221 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     2281 cgtttatata ctagagttga cagctagctc agtcctaggg actatgctag ctactagagg
     2341 ataggaggcc tttatggcct taaaagcaac agcacttttt gccatgctag gattgtcatt
     2401 tgttttatct ccatcgattg aagcgaatgt cgatcctcat tttgataaat ttatggaatc
     2461 tggtattagg cacgtttata tgctttttga aaataaaagc gtagaatcgt ctgaacaatt
     2521 ctatagtttt atgagaacga cctataaaaa tgacccgtgc tcttctgatt ttgaatgtat
     2581 agagcgaggc gcggagatgg cacaatcata cgctagaatt atgaacatta aattggagac
     2641 tgaatgaaat tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     2701 cctttcgttt tatctgttgt ttgtcggtga acgctctc
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