##gff-version 3
##sequence-region BBa_K1439010 1 2738
BBa_K1439010	.	promoter	1	35	.	+	0	ID=BBa_J23106;Name=BBa_J23106
BBa_K1439010	.	promoter	892	945	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K1439010	.	engineered_region	892	945	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1439010	.	promoter	911	916	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1439010	.	promoter	934	939	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1439010	.	non_covalent_binding_site	892	910	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1439010	.	non_covalent_binding_site	917	935	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1439010	.	engineered_region	44	883	.	+	0	ID=BBa_P0440;Name=BBa_P0440
BBa_K1439010	.	terminator	843	883	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0440
BBa_K1439010	.	stop_codon	876	876	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1439010	.	polyA_site	870	883	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1439010	.	stem_loop	850	869	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1439010	.	engineered_region	843	883	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1439010	.	terminator	755	834	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0440
BBa_K1439010	.	engineered_region	755	834	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1439010	.	stem_loop	766	809	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1439010	.	CDS	62	721	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0440
BBa_K1439010	.	engineered_tag	722	746	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K1439010	.	CDS	65	681	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K1439010	.	tag	682	715	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K1439010	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0440
BBa_K1439010	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1439010	.	engineered_region	954	2738	.	+	0	ID=BBa_K112808;Name=BBa_K112808
BBa_K1439010	.	terminator	2248	2288	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K112808
BBa_K1439010	.	stop_codon	2281	2281	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1439010	.	polyA_site	2275	2288	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1439010	.	stem_loop	2255	2274	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1439010	.	engineered_region	2248	2288	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1439010	.	promoter	2297	2331	.	+	0	ID=BBa_J23116;Name=BBa_J23116;Parent=BBa_K112808
BBa_K1439010	.	terminator	2659	2738	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K112808
BBa_K1439010	.	engineered_region	2659	2738	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1439010	.	stem_loop	2670	2713	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1439010	.	CDS	2340	2650	.	+	0	ID=BBa_K112807;Name=BBa_K112807;Parent=BBa_K112808
BBa_K1439010	.	CDS	2354	2647	.	+	0	ID=annotation1999019;Name=T4 antiholin;Parent=BBa_K112807
BBa_K1439010	.	CDS	954	1629	.	+	0	ID=BBa_K112805;Name=BBa_K112805;Parent=BBa_K112808
BBa_K1439010	.	CDS	970	1626	.	+	0	ID=annotation1999005;Name=T4 holin;Parent=BBa_K112805
BBa_K1439010	.	CDS	1638	2151	.	+	0	ID=BBa_K112806;Name=BBa_K112806;Parent=BBa_K112808
BBa_K1439010	.	CDS	1654	2148	.	+	0	ID=annotation1999018;Name=T4 Endolysin;Parent=BBa_K112806
BBa_K1439010	.	terminator	2160	2239	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K112808
BBa_K1439010	.	engineered_region	2160	2239	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1439010	.	stem_loop	2171	2214	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_K1439010
tttacggctagctcagtcctaggtatagtgctagctactagagaaagaggagaaatactagatgtccagattagataaa
agtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccaga
agctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagat
gttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagt
tttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaa
ctctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggg
gcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgat
agtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttg
aattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagt
agcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaag
actgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcc
tttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactac
tagagcttaaaaggagggtctatggcagcacctagaatatcattttcgccctctgatattctatttggtgttctcgatc
gcttgttcaaagataacgctaccgggaaggttcttgcttcccgggtagctgtcgtaattcttttgtttataatggcgat
tgtttggtataggggagatagtttctttgagtactataagcaatcaaagtatgaaacatacagtgaaattattgaaaag
gaaagaactgcacgctttgaatctgtcgccctggaacaactccagatagttcatatatcatctgaggcagactttagtg
cggtgtattctttccgccctaaaaacttaaactattttgttgatattatagcatacgaaggaaaattaccttcaacaat
aagtgaaaaatcacttggaggatatcctgttgataaaactatggatgaatatacagttcatttaaatggacgtcattat
tattccaactcaaaatttgcttttttaccaactaaaaagcctactcccgaaataaactacatgtacagttgtccatatt
ttaatttggataatatctatgctggaacgataaccatgtactggtatagaaatgatcatataagtaatgaccgccttga
atcaatatgtgctcaggcggccagaatattaggaagggctaaataattatactagagatacttaggaggtattatgaat
atatttgaaatgttacgtatagatgaaggtcttagacttaaaatctataaagacacagaaggctattacactattggca
tcggtcatttgcttacaaaaagtccatcacttaatgctgctaaatctgaattagataaagctattgggcgtaattgcaa
tggtgtaattacaaaagatgaggctgaaaaactctttaatcaggatgttgatgctgctgttcgcggaatcctgagaaat
gctaaattaaaaccggtttatgattctcttgatgcggttcgtcgctgtgcattgattaatatggttttccaaatgggag
aaaccggtgtggcaggatttactaactctttacgtatgcttcaacaaaaacgctgggatgaagcagcagttaacttagc
taaaagtagatggtataatcaaacacctaatcgcgcaaaacgagtcattacaacgtttagaactggcacttgggacgcg
tataaaaatctataaagctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttt
atctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatac
tagagttgacagctagctcagtcctagggactatgctagctactagaggataggaggcctttatggccttaaaagcaac
agcactttttgccatgctaggattgtcatttgttttatctccatcgattgaagcgaatgtcgatcctcattttgataaa
tttatggaatctggtattaggcacgtttatatgctttttgaaaataaaagcgtagaatcgtctgaacaattctatagtt
ttatgagaacgacctataaaaatgacccgtgctcttctgattttgaatgtatagagcgaggcgcggagatggcacaatc
atacgctagaattatgaacattaaattggagactgaatgaaattactagagccaggcatcaaataaaacgaaaggctca
gtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc