LOCUS BBa_K1440002 1410 bp DNA linear 25-Nov-2024 DEFINITION part1 or our whole design,SV40>PTRE>EYFP>loxP>Ribozyme>SV40 ACCESSION BBa_K1440002 VERSION BBa_K1440002.1 FEATURES Location/Qualifiers misc_feature 4..125 /label=SV40-polyA signal terminator promoter 126..403 /label=pTRE promoter CDS 409..1128 /label=EYFP misc_feature 1132..1165 /label=loxp site misc_feature 1166..1288 /label=hammerhead ribozyme misc_feature 1289..1410 /label=SV40-polyA signal terminator ORIGIN 1 atcaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 61 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 121 tcttaatcgc aggtccgagg ttctagacga gtttactccc tatcagtgat agagaacgat 181 gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga 241 tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttatc 301 cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg 361 tcgaggtagg cgtgtacggt gggaggccta tataagcaga gctgcaccat ggtgagcaag 421 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac 481 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc 541 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc 601 ttcggctacg gcctgcaatg cttcgcccgc taccccgacc acatgaagct gcacgacttc 661 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac 721 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc 781 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac 841 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg 901 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag 961 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac 1021 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc 1081 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaat cataacttcg 1141 tatagcatac attatacgaa gttatcattt accaagagac gctttttagc ctgttatctg 1201 tgggtgctac tctttcctag gagagttagc taccccacac tgatgagtcc caaaaaggac 1261 gaaataacag gctaaaaagc ctctcttgta agatacattg atgagtttgg acaaaccaca 1321 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt 1381 gtaaccatta taagctgcaa taaacaagtt //