BBa_K1440000 1 BBa_K1440000 Part2_pTRE promoter with cFP and ribozyme 2014-10-06T11:00:00Z 2015-05-08T01:10:26Z It comes from the commercial products. The ribozyme comes from a Nature paper this part is one of the regulatory parts in our whole design, it consists of pTRE promoter and cFP and ribozyme. The system can work in mammalian cells false false _1818_ 0 20183 9 It's complicated false It is a component of our whole project,can not work individually. false Xuanye Cao annotation2421238 1 SV40 polyA signal range2421238 1 1013 1134 annotation2421234 1 pTRE promoter range2421234 1 35 281 annotation2421470 1 Ribozyme range2421470 1 1135 1250 annotation2421235 1 mCFP range2421235 1 287 1006 annotation2421239 1 loxp site range2421239 1 1258 1291 BBa_K1440088 1 BBa_K1440088 long parts consist of part2 and part3 2014-10-06T11:00:00Z 2015-05-08T01:10:26Z None the part is a conjunction of part2 and part3. false false _1818_ 0 20183 9 It's complicated false None false Xuanye Cao component2421500 1 BBa_K1440000 component2421506 1 BBa_K1440066 annotation2421500 1 BBa_K1440000 range2421500 1 1 1291 annotation2421506 1 BBa_K1440066 range2421506 1 1300 2491 BBa_K1440066 1 BBa_K1440066 reverse CMV->reverse RFP->pTRE->shRNA 2014-10-06T11:00:00Z 2015-05-08T01:10:26Z It comes from a paper [Cell Cycle 8:3, 498-504; 1 February 2009]; ??2009 Landes Bioscience this part consists of reverse CMV and reverse RFP and tet-on shRFP system. With the transfection of this plasmid into the cell lines, RFP will be expressed constitutely. if dox is dosed with the cell lines, shRFP will be expressed, then knockdown the RFP expression.It must be used in mammalian cells. false false _1818_ 0 20183 9 It's complicated false RFP from the subparts may not work, because it can not be identified in our cell lines. false Xuanye Cao annotation2421448 1 H1/TO promoter range2421448 1 1019 1120 annotation2421449 1 shRFP range2421449 1 1122 1183 annotation2421442 1 mRFP range2421442 1 7 684 annotation2421444 1 SV40 polyA signal range2421444 1 894 1015 annotation2421443 1 CMV promoter range2421443 1 690 893 BBa_K1440066_sequence 1 gatatcttaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatgtccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgtcgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttcggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaactttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacacgttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttggaaccgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtctgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaactttgaaacgcatgaactctttgataacgtcttcggaggaagccatggtgcagctctgcttatatagacctcccaccgtacacgcctaccgcccatttgcgtcaatggggcggagttgttacgacattttggaaagtcccgttgattttggtgccaaaacaaactcccattgacgtcaatggggtggagacttggaaatccccgtgagtcaaaccgctatccacgcccattgatgtactgccaaaaccgcatcactaagatacattgatgagtttggacaaaccacaactagaatgcagtgaaaaaaatgctttatttgtgaaatttgtgatgctattgctttatttgtaaccattataagctgcaataaacaagttgataatatttgcatgtcgctatgtgttctgggaaatcaccataaacgtgaaatccctatcagtgatagagacttataagttccctatcagtgatagagacaccggtgatcgagttactcgggagggaaattcaagagatttccctcccgagtaactcttttttggaaagaattcgat BBa_K1440088_sequence 1 gatatcgcaggtccgaggttctagacgagtttactccctatcagtgatagagaacgatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttatccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgaggtaggcgtgtacggtgggaggcctatataagcagagctgcaccatggtatctaagggtgaagagttatttactggagtcgtgcctattctggtggagcttgatggggacgtcaacggccataaattttctgtctctggggagggagaaggcgacgcaacctatggtaagctgacgctgaagtttatttgtacgaccggcaaactccccgtaccgtggccaacgctggtcacgacgctcacgtggggcgtgcaatgtttctcgcgctatcctgaccatatgaaacagcacgactttttcaaatcggctatgccggaggggtatgtgcaagagcgcaccatctttttcaaagatgacgggaattacaaaacccgtgccgaagtcaaatttgagggtgacactcttgtcaatcgtattgaattaaagggtattgactttaaggaagatggcaatatcctcggccacaaactggaatataactacattagccacaacgtgtatattaccgcggataaacagaagaacggcattaaagctaactttaaaatccgccacaatattgaagatggcagtgtccaactcgccgatcattatcaacagaataccccgattggggatggccctgtcttactgcctgacaatcattatctgtcgacccaaagtaaactttcaaaagatccgaacgaaaaacgtgatcatatggtgttgctggagtttgtcacagcagcaggcattaccctcggcatggatgaattatacaaataataaatcaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttacaagagaggctttttagcctgttatttcgtcctttttgggactcatcagtgtggggtagctaactctcctaggaaagagtagcacccacagataacaggctaaaaagcgtctcttggtaaatgataacttcgtatagcatacattatacgaagttattactagaggatatcttaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatgtccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgtcgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttcggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaactttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacacgttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttggaaccgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtctgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaactttgaaacgcatgaactctttgataacgtcttcggaggaagccatggtgcagctctgcttatatagacctcccaccgtacacgcctaccgcccatttgcgtcaatggggcggagttgttacgacattttggaaagtcccgttgattttggtgccaaaacaaactcccattgacgtcaatggggtggagacttggaaatccccgtgagtcaaaccgctatccacgcccattgatgtactgccaaaaccgcatcactaagatacattgatgagtttggacaaaccacaactagaatgcagtgaaaaaaatgctttatttgtgaaatttgtgatgctattgctttatttgtaaccattataagctgcaataaacaagttgataatatttgcatgtcgctatgtgttctgggaaatcaccataaacgtgaaatccctatcagtgatagagacttataagttccctatcagtgatagagacaccggtgatcgagttactcgggagggaaattcaagagatttccctcccgagtaactcttttttggaaagaattcgat BBa_K1440000_sequence 1 gatatcgcaggtccgaggttctagacgagtttactccctatcagtgatagagaacgatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttatccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgaggtaggcgtgtacggtgggaggcctatataagcagagctgcaccatggtatctaagggtgaagagttatttactggagtcgtgcctattctggtggagcttgatggggacgtcaacggccataaattttctgtctctggggagggagaaggcgacgcaacctatggtaagctgacgctgaagtttatttgtacgaccggcaaactccccgtaccgtggccaacgctggtcacgacgctcacgtggggcgtgcaatgtttctcgcgctatcctgaccatatgaaacagcacgactttttcaaatcggctatgccggaggggtatgtgcaagagcgcaccatctttttcaaagatgacgggaattacaaaacccgtgccgaagtcaaatttgagggtgacactcttgtcaatcgtattgaattaaagggtattgactttaaggaagatggcaatatcctcggccacaaactggaatataactacattagccacaacgtgtatattaccgcggataaacagaagaacggcattaaagctaactttaaaatccgccacaatattgaagatggcagtgtccaactcgccgatcattatcaacagaataccccgattggggatggccctgtcttactgcctgacaatcattatctgtcgacccaaagtaaactttcaaaagatccgaacgaaaaacgtgatcatatggtgttgctggagtttgtcacagcagcaggcattaccctcggcatggatgaattatacaaataataaatcaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttacaagagaggctttttagcctgttatttcgtcctttttgggactcatcagtgtggggtagctaactctcctaggaaagagtagcacccacagataacaggctaaaaagcgtctcttggtaaatgataacttcgtatagcatacattatacgaagttat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z