BBa_K525710 1 LigA DNA ligase from Escherichia coli (LigA) with PT7, RBS and His-tag 2011-09-11T11:00:00Z 2015-05-08T01:12:35Z Escherichia coli Released HQ 2013 LigA1 mit Promotor, RBS, Histag false false _690_ 0 8543 9 In stock true PCR false Anna Drong annotation2161303 1 LigA range2161303 1 34 2045 annotation2161299 1 T7 promoter range2161299 1 1 20 annotation2161300 1 B0034 range2161300 1 21 33 annotation2161301 1 RBS range2161301 1 24 27 annotation2161302 1 ATG range2161302 1 34 36 annotation2161304 1 His-tag range2161304 1 2047 2064 BBa_K1441012 1 BBa_K1441012 DNA ligase from Escherichia coli with His-tag In pGAPz alpha A 2014-10-09T11:00:00Z 2015-05-08T01:10:26Z This part comes from a standerized IGEM biobricks PSB1C3 plasmid. This part contains the vector PGAPzα A + modified Multiple Cloning Site(MCS) fixed according to iGEM standards. We replaced the MCS with DNA ligase kit plate part(BBa_K525710). PGAPzα A + Linker has α-secretion factor before the DNA ligase kit plate part, which allows yeast cells and some Eukaryotic cells to secrete the protein outside of the cell membrane after translation. Following the insert there is a MYC epitope tag and 6x histag. These are used to tag the protein so it can be easily identified by westernblot technique. false false _1819_ 0 12451 9 In stock false The original part in the PSB1C3 vector had to be altered using PCR in order to remove the stop codons present in the IGEM standard prefixes and suffixes. false Derrica Burke component2414103 1 BBa_K525710 annotation2414103 1 BBa_K525710 range2414103 1 1 2064 BBa_K1441012_sequence 1 taatacgactcactatagggaaagaggagaaaaatggaatcaatcgaacaacaactgacagaactgcgaacgacgcttcgccatcatgaatatctttatcatgtgatggatgcgccggaaattcccgacgctgaatacgacaggctgatgcgcgaactgcgcgagctggaaaccaaacatccagaactgattacgcctgattcgcctactcaacgtgtaggcgctgcgccgctggcggctttcagccagatacgccatgaagtaccaatgctgtcactggataacgtttttgatgaagaaagctttcttgctttcaacaaacgtgtgcaggaccgtctgaaaaacaacgagaaagtcacctggtgctgtgagctgaagctggatggtcttgccgtcagtattctgtatgaaaatggcgttttagtcagtgccgcgacccgtggcgatggcaccaccggggaagatatcacgtctaatgtgcgtactattcgcgccattccgctgaagctgcacggagagaatatcccggcgcgtctggaagtgcgtggtgaagtgttcctgccgcaggcggggttcgaaaagattaacgaagatgcgcgacgcacgggcgggaaagtgtttgctaacccacgtaatgcggcagctggttcactgcgtcagcttgatccgcgtattacagcgaagcgaccgctcacttttttctgctatggcgttggtgttctggaaggtggcgagctgccggatactcatcttggccgtttactgcaatttaaaaagtgggggttgccggtcagcgatcgggtaacgctttgtgaatcggcggaagaagtgctggcgttctatcacaaagtggaagaagaccgcccgacgctgggctttgatatcgacggcgtggtgattaaggtcaactcactggcacagcaggagcagcttggctttgtcgcgcgtgccccgcgctgggcggtagcgtttaaattcccggcgcaggagcagatgacctttgtgcgtgacgtcgagtttcaggttgggcgtactggcgcgattacgcctgttgcgcgtctggaacctgtccatgttgcaggcgtgctggtgagtaacgcaaccttacacaatgcggatgaaatcgaacgtcttggtttacgcattggcgataaagtggtgattcgccgcgctggcgacgtgatcccgcaggtggttaacgtcgtgctttctgaacgcccggaagatacccgtgaggttgtattcccgacgcattgtccggtatgtggttctgacgttgagcgtgtggaaggtgaagcggttgcccgctgtaccggcggcctgatttgcggtgcgcagcgtaaagagtcgctgaaacactttgtttcccgccgtgcgatggatgttgacggaatgggcgacaaaatcatcgatcagctggttgaaaaagaatatgtccatactccggcagatctgttcaaactcaccgcaggcaaactgaccggactggagcgtatggggccaaaatcggcacaaaacgtggttaacgcgctggaaaaagcgaaagaaaccacctttgctcgcttcctctatgcacttggcatccgtgaagtcggcgaggccaccgcagcaggtctggcggcatatttcggcacgctggaagcgctggaagccgcttcgattgaagagctgcaaaaggtgcctgatgttggcattgtcgttgcatcccacgttcacaacttctttgccgaagaaagcaaccgcaatgtcatcagcgagctgttggcggaaggtgttcactggcctgcgccgatcgttatcaacgcggaagagattgacagcccgtttgctggtaaaaccgtggtgcttacgggcagcttaagccagatgtcgcgtgatgacgctaaagctcgactggtcgaactgggcgcgaaagtcgcgggcagcgtgtcgaagaaaaccgatctggtgatagcgggtgaagcggcaggatctaaactggcgaaggcgcaggaactgggcattgaagtcatcgacgaagcggaaatgctgcgtttgctgggtagccatcatcatcatcatcat BBa_K525710_sequence 1 taatacgactcactatagggaaagaggagaaaaatggaatcaatcgaacaacaactgacagaactgcgaacgacgcttcgccatcatgaatatctttatcatgtgatggatgcgccggaaattcccgacgctgaatacgacaggctgatgcgcgaactgcgcgagctggaaaccaaacatccagaactgattacgcctgattcgcctactcaacgtgtaggcgctgcgccgctggcggctttcagccagatacgccatgaagtaccaatgctgtcactggataacgtttttgatgaagaaagctttcttgctttcaacaaacgtgtgcaggaccgtctgaaaaacaacgagaaagtcacctggtgctgtgagctgaagctggatggtcttgccgtcagtattctgtatgaaaatggcgttttagtcagtgccgcgacccgtggcgatggcaccaccggggaagatatcacgtctaatgtgcgtactattcgcgccattccgctgaagctgcacggagagaatatcccggcgcgtctggaagtgcgtggtgaagtgttcctgccgcaggcggggttcgaaaagattaacgaagatgcgcgacgcacgggcgggaaagtgtttgctaacccacgtaatgcggcagctggttcactgcgtcagcttgatccgcgtattacagcgaagcgaccgctcacttttttctgctatggcgttggtgttctggaaggtggcgagctgccggatactcatcttggccgtttactgcaatttaaaaagtgggggttgccggtcagcgatcgggtaacgctttgtgaatcggcggaagaagtgctggcgttctatcacaaagtggaagaagaccgcccgacgctgggctttgatatcgacggcgtggtgattaaggtcaactcactggcacagcaggagcagcttggctttgtcgcgcgtgccccgcgctgggcggtagcgtttaaattcccggcgcaggagcagatgacctttgtgcgtgacgtcgagtttcaggttgggcgtactggcgcgattacgcctgttgcgcgtctggaacctgtccatgttgcaggcgtgctggtgagtaacgcaaccttacacaatgcggatgaaatcgaacgtcttggtttacgcattggcgataaagtggtgattcgccgcgctggcgacgtgatcccgcaggtggttaacgtcgtgctttctgaacgcccggaagatacccgtgaggttgtattcccgacgcattgtccggtatgtggttctgacgttgagcgtgtggaaggtgaagcggttgcccgctgtaccggcggcctgatttgcggtgcgcagcgtaaagagtcgctgaaacactttgtttcccgccgtgcgatggatgttgacggaatgggcgacaaaatcatcgatcagctggttgaaaaagaatatgtccatactccggcagatctgttcaaactcaccgcaggcaaactgaccggactggagcgtatggggccaaaatcggcacaaaacgtggttaacgcgctggaaaaagcgaaagaaaccacctttgctcgcttcctctatgcacttggcatccgtgaagtcggcgaggccaccgcagcaggtctggcggcatatttcggcacgctggaagcgctggaagccgcttcgattgaagagctgcaaaaggtgcctgatgttggcattgtcgttgcatcccacgttcacaacttctttgccgaagaaagcaaccgcaatgtcatcagcgagctgttggcggaaggtgttcactggcctgcgccgatcgttatcaacgcggaagagattgacagcccgtttgctggtaaaaccgtggtgcttacgggcagcttaagccagatgtcgcgtgatgacgctaaagctcgactggtcgaactgggcgcgaaagtcgcgggcagcgtgtcgaagaaaaccgatctggtgatagcgggtgaagcggcaggatctaaactggcgaaggcgcaggaactgggcattgaagtcatcgacgaagcggaaatgctgcgtttgctgggtagccatcatcatcatcatcat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z