##gff-version 3
##sequence-region BBa_K1441012 1 2064
BBa_K1441012	.	CDS	1	2064	.	+	0	ID=BBa_K525710;Name=BBa_K525710
BBa_K1441012	.	start_codon	34	36	.	+	0	ID=annotation2161302;Name=ATG;Parent=BBa_K525710
BBa_K1441012	.	ribosome_entry_site	24	27	.	+	0	ID=annotation2161301;Name=RBS;Parent=BBa_K525710
BBa_K1441012	.	engineered_region	21	33	.	+	0	ID=annotation2161300;Name=B0034;Parent=BBa_K525710
BBa_K1441012	.	promoter	1	20	.	+	0	ID=annotation2161299;Name=T7 promoter;Parent=BBa_K525710
BBa_K1441012	.	CDS	34	2045	.	+	0	ID=annotation2161303;Name=LigA;Parent=BBa_K525710
BBa_K1441012	.	tag	2047	2064	.	+	0	ID=annotation2161304;Name=His-tag;Parent=BBa_K525710
>BBa_K1441012
taatacgactcactatagggaaagaggagaaaaatggaatcaatcgaacaacaactgacagaactgcgaacgacgcttc
gccatcatgaatatctttatcatgtgatggatgcgccggaaattcccgacgctgaatacgacaggctgatgcgcgaact
gcgcgagctggaaaccaaacatccagaactgattacgcctgattcgcctactcaacgtgtaggcgctgcgccgctggcg
gctttcagccagatacgccatgaagtaccaatgctgtcactggataacgtttttgatgaagaaagctttcttgctttca
acaaacgtgtgcaggaccgtctgaaaaacaacgagaaagtcacctggtgctgtgagctgaagctggatggtcttgccgt
cagtattctgtatgaaaatggcgttttagtcagtgccgcgacccgtggcgatggcaccaccggggaagatatcacgtct
aatgtgcgtactattcgcgccattccgctgaagctgcacggagagaatatcccggcgcgtctggaagtgcgtggtgaag
tgttcctgccgcaggcggggttcgaaaagattaacgaagatgcgcgacgcacgggcgggaaagtgtttgctaacccacg
taatgcggcagctggttcactgcgtcagcttgatccgcgtattacagcgaagcgaccgctcacttttttctgctatggc
gttggtgttctggaaggtggcgagctgccggatactcatcttggccgtttactgcaatttaaaaagtgggggttgccgg
tcagcgatcgggtaacgctttgtgaatcggcggaagaagtgctggcgttctatcacaaagtggaagaagaccgcccgac
gctgggctttgatatcgacggcgtggtgattaaggtcaactcactggcacagcaggagcagcttggctttgtcgcgcgt
gccccgcgctgggcggtagcgtttaaattcccggcgcaggagcagatgacctttgtgcgtgacgtcgagtttcaggttg
ggcgtactggcgcgattacgcctgttgcgcgtctggaacctgtccatgttgcaggcgtgctggtgagtaacgcaacctt
acacaatgcggatgaaatcgaacgtcttggtttacgcattggcgataaagtggtgattcgccgcgctggcgacgtgatc
ccgcaggtggttaacgtcgtgctttctgaacgcccggaagatacccgtgaggttgtattcccgacgcattgtccggtat
gtggttctgacgttgagcgtgtggaaggtgaagcggttgcccgctgtaccggcggcctgatttgcggtgcgcagcgtaa
agagtcgctgaaacactttgtttcccgccgtgcgatggatgttgacggaatgggcgacaaaatcatcgatcagctggtt
gaaaaagaatatgtccatactccggcagatctgttcaaactcaccgcaggcaaactgaccggactggagcgtatggggc
caaaatcggcacaaaacgtggttaacgcgctggaaaaagcgaaagaaaccacctttgctcgcttcctctatgcacttgg
catccgtgaagtcggcgaggccaccgcagcaggtctggcggcatatttcggcacgctggaagcgctggaagccgcttcg
attgaagagctgcaaaaggtgcctgatgttggcattgtcgttgcatcccacgttcacaacttctttgccgaagaaagca
accgcaatgtcatcagcgagctgttggcggaaggtgttcactggcctgcgccgatcgttatcaacgcggaagagattga
cagcccgtttgctggtaaaaccgtggtgcttacgggcagcttaagccagatgtcgcgtgatgacgctaaagctcgactg
gtcgaactgggcgcgaaagtcgcgggcagcgtgtcgaagaaaaccgatctggtgatagcgggtgaagcggcaggatcta
aactggcgaaggcgcaggaactgggcattgaagtcatcgacgaagcggaaatgctgcgtttgctgggtagccatcatca
tcatcatcat