LOCUS       BBa_K1442102             892 bp    DNA     linear       22-Nov-2024
DEFINITION  Reverse GFP for E.coli
ACCESSION   BBa_K1442102
VERSION     BBa_K1442102.1  
FEATURES             Location/Qualifiers
     promoter        1..27
                     /label=BBa_K1442029
     CDS             28..717
                     /label=GFP codon optimised for E.coli
     CDS             28..717
                     /label=BBa_K1442302
     misc_feature    722..725
                     /label=conserved
     RBS             718..729
                     /label=BBa_B0034
     misc_feature    730..735
                     /label=AflII restriction site 
     stem_loop       736..768
                     /label=SLD3
     misc_feature    769..840
                     /label=Hammerhead Ribozyme
     misc_feature    841..846
                     /label=BsiWI restriction site
     promoter        730..846
                     /label=BBa_K1442304
     terminator      847..892
                     /label=BBa_K1442301
ORIGIN
        1 gcgaaattaa tacgactcac tatagggcgt aatgccagcc gcagtgacaa attccagaag
       61 caccatgtga tcgcgcttct cgttaggatc tttactcagc gcgctctgtg ttgataagta
      121 atggttatcc ggcaacagga cgggaccatc accgataggg gtattctgtt ggtaatgatc
      181 tgccaactgc acgctgccat cttcaatgtt atggcgaatt ttaaaattca ctttgatacc
      241 atttttctgc ttgtccgcca tgatgtacac gttgtgggaa ttatagttgt actccagttt
      301 atggcccagg atattgccgt cctctttaaa atcaatgcct ttcagctcga tacgattcac
      361 cagggtatca ccttcgaatt taacttcagc ccgagtctta taattaccat cgtctttaaa
      421 gaaaatggtg cgttcctgca cataaccctc cggcattgcc gacttaaaaa aatcgtgctg
      481 cttcatgtga tccgggtagc gtgcaaaaca ctgaaccccg taaccaaagg ttgttaccaa
      541 ggtcggccat ggcaccggca gcttacccgt ggtacaaatg aatttcaggg tcaatttccc
      601 ataggtggcg tcaccttctc cttcgccaga gactgaaaat ttatggccat tcacgtcgcc
      661 gtcgagttcc acaagaatag gcacaacgcc ggtaaacagt tcttcgcctt tacgcataaa
      721 gaggagaaac ttaaggggcg cgccggcttg catagcaagt ctgagaccgg ccggcatggt
      781 cccagcctcc tcgctggcgc cggctgggca acaccattgc actccggtgg cgaatgggac
      841 cgtacgtagc ataacccctt ggggcctcta aacgggtctt gaggggtttt tt
//