LOCUS       BBa_K1442106             872 bp    DNA     linear       16-Jun-2024
DEFINITION  Forward GFP for human cells
ACCESSION   BBa_K1442106
VERSION     BBa_K1442106.1  
FEATURES             Location/Qualifiers
     promoter        1..27
                     /label=BBa_K1442029
     misc_feature    36..43
                     /label=AscI resctriction site
     misc_feature    56..61
                     /label=BsrGI restriction site 
     CDS             62..781
                     /label=GFP codon optimised for humans
     misc_feature    776..778
                     /label=Stop codon
     misc_feature    779..781
                     /label=Stop codon
     misc_feature    782..789
                     /label=MauBI restriciton site 
     misc_feature    802..807
                     /label=MluI
     polyA_site      808..818
                     /label=poly A tail 
     CDS             36..818
                     /label=BBa_K1442308
     terminator      827..872
                     /label=BBa_K1442301
ORIGIN
        1 gcgaaattaa tacgactcac tatagggtac tagagggcgc gccatcgagt tatgctgtac
       61 aatgcggaaa ggggaggagc tcttcacagg cgtggtgccc atactggtgg agttggacgg
      121 cgacgtgaac ggtcacaagt tctcagtctc tggggaggga gagggcgatg ccacctatgg
      181 caagcttaca ctgaagttca tttgtacgac cggcaagctc cctgtgccat ggcccaccct
      241 ggtcacaacc ttcggctacg gggtgcagtg ctttgccaga tatcccgacc acatgaagca
      301 gcatgacttc tttaagagtg caatgcccga agggtatgtg caggaacgaa cgattttttt
      361 taaggacgac gggaattaca aaacccgcgc cgaggttaag ttcgagggcg ataccctggt
      421 taatcggata gagctcaaag gaatagattt taaagaggac ggtaacattc tgggccacaa
      481 gctcgaatat aactataatt cacacaacgt atacatcatg gctgacaagc agaaaaacgg
      541 catcaaagtt aactttaaga tacgacacaa cattgaagac gggtctgtgc agttggctga
      601 ccactaccag cagaacacgc cgatcgggga tggaccggtg ctgctgcctg acaaccacta
      661 cctctctacc cagtctgccc tgtctaagga ccctaacgaa aagcgggatc acatggtgct
      721 tcttgagttc gtcactgccg caggaattac acacggtatg gacgaactct ataagtaata
      781 acgcgcgcga ctgaatgcat gacgcgtaaa aaaaaaaata ctagagtagc ataacccctt
      841 ggggcctcta aacgggtctt gaggggtttt tt
//