LOCUS       BBa_K1442109            1432 bp    DNA     linear       22-Nov-2024
DEFINITION  EMCV IRES with forward GFP
ACCESSION   BBa_K1442109
VERSION     BBa_K1442109.1  
FEATURES             Location/Qualifiers
     promoter        1..27
                     /label=BBa_K1442029
     misc_feature    28..35
                     /label=AscI restriction site
     RBS             36..646
                     /label=EMCV internal Ribosome Entry SIte 
     misc_feature    647..652
                     /label=BsrGI restriction site
     RBS             28..652
                     /label=BBa_K1442023
     promoter        653..679
                     /label=BBa_K1442029
     misc_feature    688..695
                     /label=AscI resctriction site
     misc_feature    708..713
                     /label=BsrGI restriction site 
     CDS             714..1433
                     /label=GFP codon optimised for humans
     misc_feature    1428..1430
                     /label=Stop codon
     misc_feature    1431..1433
                     /label=Stop codon
     misc_feature    1434..1441
                     /label=MauBI restriciton site 
     misc_feature    1454..1459
                     /label=MluI
     polyA_site      1460..1470
                     /label=poly A tail 
     CDS             688..1470
                     /label=BBa_K1442308
     terminator      1479..1524
                     /label=BBa_K1442301
     misc_feature    653..1386
                     /label=BBa_K1442106
     terminator      1387..1432
                     /label=BBa_K1442301
ORIGIN
        1 gcgaaattaa tacgactcac tatagggggc gcgccagacc acaacggttt ccctctagcg
       61 ggatcaattc cgcccctctc cctccccccc cggtaacgtt actggccgaa gccgcttgga
      121 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa
      181 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc
      241 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc
      301 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg
      361 cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca
      421 accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag
      481 cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct
      541 ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaacg tctaggcccc
      601 ccgaaccacg gggacgtggt tttcctttga aaaacacgat aatacctgta catgtacaat
      661 gcggaaaggg gaggagctct tcacaggcgt ggtgcccata ctggtggagt tggacggcga
      721 cgtgaacggt cacaagttct cagtctctgg ggagggagag ggcgatgcca cctatggcaa
      781 gcttacactg aagttcattt gtacgaccgg caagctccct gtgccatggc ccaccctggt
      841 cacaaccttc ggctacgggg tgcagtgctt tgccagatat cccgaccaca tgaagcagca
      901 tgacttcttt aagagtgcaa tgcccgaagg gtatgtgcag gaacgaacga ttttttttaa
      961 ggacgacggg aattacaaaa cccgcgccga ggttaagttc gagggcgata ccctggttaa
     1021 tcggatagag ctcaaaggaa tagattttaa agaggacggt aacattctgg gccacaagct
     1081 cgaatataac tataattcac acaacgtata catcatggct gacaagcaga aaaacggcat
     1141 caaagttaac tttaagatac gacacaacat tgaagacggg tctgtgcagt tggctgacca
     1201 ctaccagcag aacacgccga tcggggatgg accggtgctg ctgcctgaca accactacct
     1261 ctctacccag tctgccctgt ctaaggaccc taacgaaaag cgggatcaca tggtgcttct
     1321 tgagttcgtc actgccgcag gaattacaca cggtatggac gaactctata agtaataacg
     1381 cgcgcgtagc ataacccctt ggggcctcta aacgggtctt gaggggtttt tt
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