##gff-version 3
##sequence-region BBa_K1442109 1 1432
BBa_K1442109	.	ribosome_entry_site	28	652	.	+	0	ID=BBa_K1442023;Name=BBa_K1442023
BBa_K1442109	.	ribosome_entry_site	36	646	.	+	0	ID=annotation2396242;Name=EMCV internal Ribosome Entry SIte ;Parent=BBa_K1442023
BBa_K1442109	.	sequence_feature	28	35	.	+	0	ID=annotation2396241;Name=AscI restriction site;Parent=BBa_K1442023
BBa_K1442109	.	sequence_feature	647	652	.	+	0	ID=annotation2396273;Name=BsrGI restriction site;Parent=BBa_K1442023
BBa_K1442109	.	terminator	1387	1432	.	+	0	ID=BBa_K1442301;Name=BBa_K1442301
BBa_K1442109	.	promoter	1	27	.	+	0	ID=BBa_K1442029;Name=BBa_K1442029
BBa_K1442109	.	engineered_region	653	1386	.	+	0	ID=BBa_K1442106;Name=BBa_K1442106
BBa_K1442109	.	promoter	653	679	.	+	0	ID=BBa_K1442029;Name=BBa_K1442029;Parent=BBa_K1442106
BBa_K1442109	.	CDS	688	1470	.	+	0	ID=BBa_K1442308;Name=BBa_K1442308;Parent=BBa_K1442106
BBa_K1442109	.	polyA_site	1460	1470	.	+	0	ID=annotation2420100;Name=poly A tail ;Parent=BBa_K1442308
BBa_K1442109	.	stop_codon	1431	1433	.	+	0	ID=annotation2420096;Name=Stop codon;Parent=BBa_K1442308
BBa_K1442109	.	sequence_feature	1454	1459	.	+	0	ID=annotation2420098;Name=MluI;Parent=BBa_K1442308
BBa_K1442109	.	sequence_feature	1434	1441	.	+	0	ID=annotation2420097;Name=MauBI restriciton site ;Parent=BBa_K1442308
BBa_K1442109	.	CDS	714	1433	.	+	0	ID=annotation2420094;Name=GFP codon optimised for humans;Parent=BBa_K1442308
BBa_K1442109	.	sequence_feature	708	713	.	+	0	ID=annotation2420093;Name=BsrGI restriction site ;Parent=BBa_K1442308
BBa_K1442109	.	stop_codon	1428	1430	.	+	0	ID=annotation2420095;Name=Stop codon;Parent=BBa_K1442308
BBa_K1442109	.	sequence_feature	688	695	.	+	0	ID=annotation2420092;Name=AscI resctriction site;Parent=BBa_K1442308
BBa_K1442109	.	terminator	1479	1524	.	+	0	ID=BBa_K1442301;Name=BBa_K1442301;Parent=BBa_K1442106
>BBa_K1442109
gcgaaattaatacgactcactatagggggcgcgccagaccacaacggtttccctctagcgggatcaattccgcccctct
ccctccccccccggtaacgttactggccgaagccgcttggaataaggccggtgtgcgtttgtctatatgttattttcca
ccatattgccgtcttttggcaatgtgagggcccggaaacctggccctgtcttcttgacgagcattcctaggggtctttc
ccctctcgccaaaggaatgcaaggtctgttgaatgtcgtgaaggaagcagttcctctggaagcttcttgaagacaaaca
acgtctgtagcgaccctttgcaggcagcggaaccccccacctggcgacaggtgcctctgcggccaaaagccacgtgtat
aagatacacctgcaaaggcggcacaaccccagtgccacgttgtgagttggatagttgtggaaagagtcaaatggctctc
ctcaagcgtattcaacaaggggctgaaggatgcccagaaggtaccccattgtatgggatctgatctggggcctcggtgc
acatgctttacatgtgtttagtcgaggttaaaaaacgtctaggccccccgaaccacggggacgtggttttcctttgaaa
aacacgataatacctgtacatgtacaatgcggaaaggggaggagctcttcacaggcgtggtgcccatactggtggagtt
ggacggcgacgtgaacggtcacaagttctcagtctctggggagggagagggcgatgccacctatggcaagcttacactg
aagttcatttgtacgaccggcaagctccctgtgccatggcccaccctggtcacaaccttcggctacggggtgcagtgct
ttgccagatatcccgaccacatgaagcagcatgacttctttaagagtgcaatgcccgaagggtatgtgcaggaacgaac
gattttttttaaggacgacgggaattacaaaacccgcgccgaggttaagttcgagggcgataccctggttaatcggata
gagctcaaaggaatagattttaaagaggacggtaacattctgggccacaagctcgaatataactataattcacacaacg
tatacatcatggctgacaagcagaaaaacggcatcaaagttaactttaagatacgacacaacattgaagacgggtctgt
gcagttggctgaccactaccagcagaacacgccgatcggggatggaccggtgctgctgcctgacaaccactacctctct
acccagtctgccctgtctaaggaccctaacgaaaagcgggatcacatggtgcttcttgagttcgtcactgccgcaggaa
ttacacacggtatggacgaactctataagtaataacgcgcgcgtagcataaccccttggggcctctaaacgggtcttga
ggggtttttt