BBa_K1442029 1 BBa_K1442029 T7 Promoter Soler Bistue 2014-09-03T11:00:00Z 2015-05-08T01:10:26Z Sequence obtained from Soler Bistu??. Antimicrob. Agents Chemother. 2007, 51:1918 The T7 promoter is used to initiate transcription when placed at the 5' end of a DNA sequence false false _1820_ 0 23056 9 Not in stock false 3 Gs at the end are essential for T7 RNAP false Caroline de Cock BBa_K1442109 1 EMCV/FG EMCV IRES with forward GFP 2014-10-06T11:00:00Z 2015-05-08T01:10:27Z ... ... false false _1820_ 0 23055 9 It's complicated true ... false Caroline de Cock component2417993 1 BBa_K1442029 component2417997 1 BBa_K1442023 component2418000 1 BBa_K1442301 component2417999 1 BBa_K1442106 annotation2417993 1 BBa_K1442029 range2417993 1 1 27 annotation2417997 1 BBa_K1442023 range2417997 1 28 652 annotation2417999 1 BBa_K1442106 range2417999 1 653 1386 annotation2418000 1 BBa_K1442301 range2418000 1 1387 1432 BBa_K1442301 1 BBa_K1442301 T7 terminator Soler Bistue 2014-10-12T11:00:00Z 2015-05-08T01:10:27Z http://www.plosone.org/article/fetchObject.action?uri=info%3Adoi%2F10.1371%2Fjournal.pone.0047690&representation=PDF T7 terminator Soler Bistue. Terminates transpcription when added at the 3' end of a sequence false false _1820_ 0 21957 9 Not in stock true None false Caroline de Cock BBa_K1442308 1 BBa_K1442308 GFP codon optimised for human cells 2014-10-14T11:00:00Z 2015-05-08T01:10:27Z Part BBa_E0040 GFP codon optimised for use in human cells derived from the Aequeora victoria GFP and codon optimised using IDT codon optimiser. false false _1820_ 0 21957 9 Not in stock false Codon optimised false Caroline de Cock annotation2420100 1 poly A tail range2420100 1 773 783 annotation2420096 1 Stop codon range2420096 1 744 746 annotation2420098 1 MluI range2420098 1 767 772 annotation2420097 1 MauBI restriciton site range2420097 1 747 754 annotation2420093 1 BsrGI restriction site range2420093 1 21 26 annotation2420094 1 GFP codon optimised for humans range2420094 1 27 746 annotation2420095 1 Stop codon range2420095 1 741 743 annotation2420092 1 AscI resctriction site range2420092 1 1 8 BBa_K1442106 1 FG Forward GFP for human cells 2014-10-06T11:00:00Z 2015-05-08T01:10:27Z ... Forward GFP for use in human cell tests. false false _1820_ 0 23055 9 It's complicated true ... false Dan Goss component2420111 1 BBa_K1442301 component2420110 1 BBa_K1442308 component2420101 1 BBa_K1442029 annotation2420110 1 BBa_K1442308 range2420110 1 36 818 annotation2420101 1 BBa_K1442029 range2420101 1 1 27 annotation2420111 1 BBa_K1442301 range2420111 1 827 872 BBa_K1442023 1 EMCV EMCV IRES 2014-08-27T11:00:00Z 2015-05-08T01:10:26Z The IRES came from the EMCV virus. EMCV IRES can be used in mammalian cells to induce translation of a protein-coding RNA when placed on the 5' end of the sequence. false false _1820_ 0 23056 9 In stock true The EMCV IRES had to be made. false Caroline de Cock annotation2396241 1 AscI restriction site range2396241 1 1 8 annotation2396273 1 BsrGI restriction site range2396273 1 620 625 annotation2396242 1 EMCV internal Ribosome Entry SIte range2396242 1 9 619 BBa_K1442301_sequence 1 tagcataaccccttggggcctctaaacgggtcttgaggggtttttt BBa_K1442308_sequence 1 ggcgcgccatcgagttatgctgtacaatgcggaaaggggaggagctcttcacaggcgtggtgcccatactggtggagttggacggcgacgtgaacggtcacaagttctcagtctctggggagggagagggcgatgccacctatggcaagcttacactgaagttcatttgtacgaccggcaagctccctgtgccatggcccaccctggtcacaaccttcggctacggggtgcagtgctttgccagatatcccgaccacatgaagcagcatgacttctttaagagtgcaatgcccgaagggtatgtgcaggaacgaacgattttttttaaggacgacgggaattacaaaacccgcgccgaggttaagttcgagggcgataccctggttaatcggatagagctcaaaggaatagattttaaagaggacggtaacattctgggccacaagctcgaatataactataattcacacaacgtatacatcatggctgacaagcagaaaaacggcatcaaagttaactttaagatacgacacaacattgaagacgggtctgtgcagttggctgaccactaccagcagaacacgccgatcggggatggaccggtgctgctgcctgacaaccactacctctctacccagtctgccctgtctaaggaccctaacgaaaagcgggatcacatggtgcttcttgagttcgtcactgccgcaggaattacacacggtatggacgaactctataagtaataacgcgcgcgactgaatgcatgacgcgtaaaaaaaaaaa BBa_K1442029_sequence 1 gcgaaattaatacgactcactataggg BBa_K1442023_sequence 1 ggcgcgccagaccacaacggtttccctctagcgggatcaattccgcccctctccctccccccccggtaacgttactggccgaagccgcttggaataaggccggtgtgcgtttgtctatatgttattttccaccatattgccgtcttttggcaatgtgagggcccggaaacctggccctgtcttcttgacgagcattcctaggggtctttcccctctcgccaaaggaatgcaaggtctgttgaatgtcgtgaaggaagcagttcctctggaagcttcttgaagacaaacaacgtctgtagcgaccctttgcaggcagcggaaccccccacctggcgacaggtgcctctgcggccaaaagccacgtgtataagatacacctgcaaaggcggcacaaccccagtgccacgttgtgagttggatagttgtggaaagagtcaaatggctctcctcaagcgtattcaacaaggggctgaaggatgcccagaaggtaccccattgtatgggatctgatctggggcctcggtgcacatgctttacatgtgtttagtcgaggttaaaaaacgtctaggccccccgaaccacggggacgtggttttcctttgaaaaacacgataatacctgtaca BBa_K1442106_sequence 1 gcgaaattaatacgactcactatagggtactagagggcgcgccatcgagttatgctgtacaatgcggaaaggggaggagctcttcacaggcgtggtgcccatactggtggagttggacggcgacgtgaacggtcacaagttctcagtctctggggagggagagggcgatgccacctatggcaagcttacactgaagttcatttgtacgaccggcaagctccctgtgccatggcccaccctggtcacaaccttcggctacggggtgcagtgctttgccagatatcccgaccacatgaagcagcatgacttctttaagagtgcaatgcccgaagggtatgtgcaggaacgaacgattttttttaaggacgacgggaattacaaaacccgcgccgaggttaagttcgagggcgataccctggttaatcggatagagctcaaaggaatagattttaaagaggacggtaacattctgggccacaagctcgaatataactataattcacacaacgtatacatcatggctgacaagcagaaaaacggcatcaaagttaactttaagatacgacacaacattgaagacgggtctgtgcagttggctgaccactaccagcagaacacgccgatcggggatggaccggtgctgctgcctgacaaccactacctctctacccagtctgccctgtctaaggaccctaacgaaaagcgggatcacatggtgcttcttgagttcgtcactgccgcaggaattacacacggtatggacgaactctataagtaataacgcgcgcgactgaatgcatgacgcgtaaaaaaaaaaatactagagtagcataaccccttggggcctctaaacgggtcttgaggggtttttt BBa_K1442109_sequence 1 gcgaaattaatacgactcactatagggggcgcgccagaccacaacggtttccctctagcgggatcaattccgcccctctccctccccccccggtaacgttactggccgaagccgcttggaataaggccggtgtgcgtttgtctatatgttattttccaccatattgccgtcttttggcaatgtgagggcccggaaacctggccctgtcttcttgacgagcattcctaggggtctttcccctctcgccaaaggaatgcaaggtctgttgaatgtcgtgaaggaagcagttcctctggaagcttcttgaagacaaacaacgtctgtagcgaccctttgcaggcagcggaaccccccacctggcgacaggtgcctctgcggccaaaagccacgtgtataagatacacctgcaaaggcggcacaaccccagtgccacgttgtgagttggatagttgtggaaagagtcaaatggctctcctcaagcgtattcaacaaggggctgaaggatgcccagaaggtaccccattgtatgggatctgatctggggcctcggtgcacatgctttacatgtgtttagtcgaggttaaaaaacgtctaggccccccgaaccacggggacgtggttttcctttgaaaaacacgataatacctgtacatgtacaatgcggaaaggggaggagctcttcacaggcgtggtgcccatactggtggagttggacggcgacgtgaacggtcacaagttctcagtctctggggagggagagggcgatgccacctatggcaagcttacactgaagttcatttgtacgaccggcaagctccctgtgccatggcccaccctggtcacaaccttcggctacggggtgcagtgctttgccagatatcccgaccacatgaagcagcatgacttctttaagagtgcaatgcccgaagggtatgtgcaggaacgaacgattttttttaaggacgacgggaattacaaaacccgcgccgaggttaagttcgagggcgataccctggttaatcggatagagctcaaaggaatagattttaaagaggacggtaacattctgggccacaagctcgaatataactataattcacacaacgtatacatcatggctgacaagcagaaaaacggcatcaaagttaactttaagatacgacacaacattgaagacgggtctgtgcagttggctgaccactaccagcagaacacgccgatcggggatggaccggtgctgctgcctgacaaccactacctctctacccagtctgccctgtctaaggaccctaacgaaaagcgggatcacatggtgcttcttgagttcgtcactgccgcaggaattacacacggtatggacgaactctataagtaataacgcgcgcgtagcataaccccttggggcctctaaacgggtcttgaggggtttttt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z