LOCUS       BBa_K1442110            1498 bp    DNA     linear       27-Dec-2024
DEFINITION  NKRF IRES with forward GFP
ACCESSION   BBa_K1442110
VERSION     BBa_K1442110.1  
FEATURES             Location/Qualifiers
     promoter        1..27
                     /label=BBa_K1442029
     misc_feature    36..43
                     /label=AscI restriction site 
     RBS             44..696
                     /label=NKRF IRES
     misc_feature    697..702
                     /label=BsrGI restriction site 
     RBS             36..702
                     /label=BBa_K1442024
     promoter        711..737
                     /label=BBa_K1442029
     misc_feature    746..753
                     /label=AscI resctriction site
     misc_feature    766..771
                     /label=BsrGI restriction site 
     CDS             772..1491
                     /label=GFP codon optimised for humans
     misc_feature    1486..1488
                     /label=Stop codon
     misc_feature    1489..1491
                     /label=Stop codon
     misc_feature    1492..1499
                     /label=MauBI restriciton site 
     misc_feature    1512..1517
                     /label=MluI
     polyA_site      1518..1528
                     /label=poly A tail 
     CDS             746..1528
                     /label=BBa_K1442308
     terminator      1537..1582
                     /label=BBa_K1442301
     misc_feature    711..1444
                     /label=BBa_K1442106
     terminator      1453..1498
                     /label=BBa_K1442301
ORIGIN
        1 gcgaaattaa tacgactcac tatagggtac tagagggcgc gcccagagta atgacatggt
       61 tccttccatc ctccaaaggt gaccaataat agtttgtaag tatcattatg aactaatgaa
      121 ttttcaacat atttgatata tttcaatcca ttgccatcat tgttcttatc gatatttgag
      181 ttggctcact ttgccagtaa gagtctattc aaattggctt ctgagtccat ttgacacaac
      241 acctttgatc tttgacagtt tccttggttt taggtgctag atgatttctc aggctcacct
      301 tagacatttc ctgccacaga cttagaatca gccatttctc taaggaccct gattccattt
      361 catgagaaat gatagagacc acaatcaaaa caagtcatga atttatactg atattttcaa
      421 ttcaaattaa agatgaggtt tttgctaaat ttttttgagt ttatatttgt atgtcttatg
      481 ctgaaaaatc ttgtttccta attagtaaca taattattca tttgatgggt aaatatttta
      541 gggccgattc tttggtttta tagccaagat accctgttga taaagtcttg tgggagcaat
      601 tataagactg gcttattttg aagcttttta aaaaagacat ccttacctgt tttaactgta
      661 gattatatta acttaaatag gtacagccca cgcttgtgta catactagag tgtacaatgc
      721 ggaaagggga ggagctcttc acaggcgtgg tgcccatact ggtggagttg gacggcgacg
      781 tgaacggtca caagttctca gtctctgggg agggagaggg cgatgccacc tatggcaagc
      841 ttacactgaa gttcatttgt acgaccggca agctccctgt gccatggccc accctggtca
      901 caaccttcgg ctacggggtg cagtgctttg ccagatatcc cgaccacatg aagcagcatg
      961 acttctttaa gagtgcaatg cccgaagggt atgtgcagga acgaacgatt ttttttaagg
     1021 acgacgggaa ttacaaaacc cgcgccgagg ttaagttcga gggcgatacc ctggttaatc
     1081 ggatagagct caaaggaata gattttaaag aggacggtaa cattctgggc cacaagctcg
     1141 aatataacta taattcacac aacgtataca tcatggctga caagcagaaa aacggcatca
     1201 aagttaactt taagatacga cacaacattg aagacgggtc tgtgcagttg gctgaccact
     1261 accagcagaa cacgccgatc ggggatggac cggtgctgct gcctgacaac cactacctct
     1321 ctacccagtc tgccctgtct aaggacccta acgaaaagcg ggatcacatg gtgcttcttg
     1381 agttcgtcac tgccgcagga attacacacg gtatggacga actctataag taataacgcg
     1441 cgcgtactag agtagcataa ccccttgggg cctctaaacg ggtcttgagg ggtttttt
//