BBa_K1442029 1 BBa_K1442029 T7 Promoter Soler Bistue 2014-09-03T11:00:00Z 2015-05-08T01:10:26Z Sequence obtained from Soler Bistu??. Antimicrob. Agents Chemother. 2007, 51:1918 The T7 promoter is used to initiate transcription when placed at the 5' end of a DNA sequence false false _1820_ 0 23056 9 Not in stock false 3 Gs at the end are essential for T7 RNAP false Caroline de Cock BBa_K1442106 1 FG Forward GFP for human cells 2014-10-06T11:00:00Z 2015-05-08T01:10:27Z ... Forward GFP for use in human cell tests. false false _1820_ 0 23055 9 It's complicated true ... false Dan Goss component2420110 1 BBa_K1442308 component2420101 1 BBa_K1442029 component2420111 1 BBa_K1442301 annotation2420110 1 BBa_K1442308 range2420110 1 36 818 annotation2420101 1 BBa_K1442029 range2420101 1 1 27 annotation2420111 1 BBa_K1442301 range2420111 1 827 872 BBa_K1442301 1 BBa_K1442301 T7 terminator Soler Bistue 2014-10-12T11:00:00Z 2015-05-08T01:10:27Z http://www.plosone.org/article/fetchObject.action?uri=info%3Adoi%2F10.1371%2Fjournal.pone.0047690&representation=PDF T7 terminator Soler Bistue. Terminates transpcription when added at the 3' end of a sequence false false _1820_ 0 21957 9 Not in stock true None false Caroline de Cock BBa_K1442110 1 NKRF/FG NKRF IRES with forward GFP 2014-10-06T11:00:00Z 2015-05-08T01:10:27Z ... ... false false _1820_ 0 23055 9 It's complicated true ... false Caroline de Cock component2417977 1 BBa_K1442029 component2417984 1 BBa_K1442301 component2417981 1 BBa_K1442024 component2417983 1 BBa_K1442106 annotation2417977 1 BBa_K1442029 range2417977 1 1 27 annotation2417981 1 BBa_K1442024 range2417981 1 36 702 annotation2417984 1 BBa_K1442301 range2417984 1 1453 1498 annotation2417983 1 BBa_K1442106 range2417983 1 711 1444 BBa_K1442024 1 NKRF NKRF IRES 2014-08-27T11:00:00Z 2015-05-08T01:10:26Z NF-kappa-B-repressing factor (NKRF) is derived from a human source, the IRES was therefore derived from a human gene. The NFRF IRES may be used to induce expression of a protein-coding RNA when placed on the 5' end of a RNA sequence false false _1820_ 0 23056 9 In stock true None. false Caroline de Cock annotation2399343 1 NKRF IRES range2399343 1 9 661 annotation2399332 1 AscI restriction site range2399332 1 1 8 annotation2399344 1 BsrGI restriction site range2399344 1 662 667 BBa_K1442308 1 BBa_K1442308 GFP codon optimised for human cells 2014-10-14T11:00:00Z 2015-05-08T01:10:27Z Part BBa_E0040 GFP codon optimised for use in human cells derived from the Aequeora victoria GFP and codon optimised using IDT codon optimiser. false false _1820_ 0 21957 9 Not in stock false Codon optimised false Caroline de Cock annotation2420100 1 poly A tail range2420100 1 773 783 annotation2420095 1 Stop codon range2420095 1 741 743 annotation2420097 1 MauBI restriciton site range2420097 1 747 754 annotation2420096 1 Stop codon range2420096 1 744 746 annotation2420094 1 GFP codon optimised for humans range2420094 1 27 746 annotation2420093 1 BsrGI restriction site range2420093 1 21 26 annotation2420092 1 AscI resctriction site range2420092 1 1 8 annotation2420098 1 MluI range2420098 1 767 772 BBa_K1442024_sequence 1 ggcgcgcccagagtaatgacatggttccttccatcctccaaaggtgaccaataatagtttgtaagtatcattatgaactaatgaattttcaacatatttgatatatttcaatccattgccatcattgttcttatcgatatttgagttggctcactttgccagtaagagtctattcaaattggcttctgagtccatttgacacaacacctttgatctttgacagtttccttggttttaggtgctagatgatttctcaggctcaccttagacatttcctgccacagacttagaatcagccatttctctaaggaccctgattccatttcatgagaaatgatagagaccacaatcaaaacaagtcatgaatttatactgatattttcaattcaaattaaagatgaggtttttgctaaatttttttgagtttatatttgtatgtcttatgctgaaaaatcttgtttcctaattagtaacataattattcatttgatgggtaaatattttagggccgattctttggttttatagccaagataccctgttgataaagtcttgtgggagcaattataagactggcttattttgaagctttttaaaaaagacatccttacctgttttaactgtagattatattaacttaaataggtacagcccacgcttgtgtaca BBa_K1442301_sequence 1 tagcataaccccttggggcctctaaacgggtcttgaggggtttttt BBa_K1442308_sequence 1 ggcgcgccatcgagttatgctgtacaatgcggaaaggggaggagctcttcacaggcgtggtgcccatactggtggagttggacggcgacgtgaacggtcacaagttctcagtctctggggagggagagggcgatgccacctatggcaagcttacactgaagttcatttgtacgaccggcaagctccctgtgccatggcccaccctggtcacaaccttcggctacggggtgcagtgctttgccagatatcccgaccacatgaagcagcatgacttctttaagagtgcaatgcccgaagggtatgtgcaggaacgaacgattttttttaaggacgacgggaattacaaaacccgcgccgaggttaagttcgagggcgataccctggttaatcggatagagctcaaaggaatagattttaaagaggacggtaacattctgggccacaagctcgaatataactataattcacacaacgtatacatcatggctgacaagcagaaaaacggcatcaaagttaactttaagatacgacacaacattgaagacgggtctgtgcagttggctgaccactaccagcagaacacgccgatcggggatggaccggtgctgctgcctgacaaccactacctctctacccagtctgccctgtctaaggaccctaacgaaaagcgggatcacatggtgcttcttgagttcgtcactgccgcaggaattacacacggtatggacgaactctataagtaataacgcgcgcgactgaatgcatgacgcgtaaaaaaaaaaa BBa_K1442029_sequence 1 gcgaaattaatacgactcactataggg BBa_K1442110_sequence 1 gcgaaattaatacgactcactatagggtactagagggcgcgcccagagtaatgacatggttccttccatcctccaaaggtgaccaataatagtttgtaagtatcattatgaactaatgaattttcaacatatttgatatatttcaatccattgccatcattgttcttatcgatatttgagttggctcactttgccagtaagagtctattcaaattggcttctgagtccatttgacacaacacctttgatctttgacagtttccttggttttaggtgctagatgatttctcaggctcaccttagacatttcctgccacagacttagaatcagccatttctctaaggaccctgattccatttcatgagaaatgatagagaccacaatcaaaacaagtcatgaatttatactgatattttcaattcaaattaaagatgaggtttttgctaaatttttttgagtttatatttgtatgtcttatgctgaaaaatcttgtttcctaattagtaacataattattcatttgatgggtaaatattttagggccgattctttggttttatagccaagataccctgttgataaagtcttgtgggagcaattataagactggcttattttgaagctttttaaaaaagacatccttacctgttttaactgtagattatattaacttaaataggtacagcccacgcttgtgtacatactagagtgtacaatgcggaaaggggaggagctcttcacaggcgtggtgcccatactggtggagttggacggcgacgtgaacggtcacaagttctcagtctctggggagggagagggcgatgccacctatggcaagcttacactgaagttcatttgtacgaccggcaagctccctgtgccatggcccaccctggtcacaaccttcggctacggggtgcagtgctttgccagatatcccgaccacatgaagcagcatgacttctttaagagtgcaatgcccgaagggtatgtgcaggaacgaacgattttttttaaggacgacgggaattacaaaacccgcgccgaggttaagttcgagggcgataccctggttaatcggatagagctcaaaggaatagattttaaagaggacggtaacattctgggccacaagctcgaatataactataattcacacaacgtatacatcatggctgacaagcagaaaaacggcatcaaagttaactttaagatacgacacaacattgaagacgggtctgtgcagttggctgaccactaccagcagaacacgccgatcggggatggaccggtgctgctgcctgacaaccactacctctctacccagtctgccctgtctaaggaccctaacgaaaagcgggatcacatggtgcttcttgagttcgtcactgccgcaggaattacacacggtatggacgaactctataagtaataacgcgcgcgtactagagtagcataaccccttggggcctctaaacgggtcttgaggggtttttt BBa_K1442106_sequence 1 gcgaaattaatacgactcactatagggtactagagggcgcgccatcgagttatgctgtacaatgcggaaaggggaggagctcttcacaggcgtggtgcccatactggtggagttggacggcgacgtgaacggtcacaagttctcagtctctggggagggagagggcgatgccacctatggcaagcttacactgaagttcatttgtacgaccggcaagctccctgtgccatggcccaccctggtcacaaccttcggctacggggtgcagtgctttgccagatatcccgaccacatgaagcagcatgacttctttaagagtgcaatgcccgaagggtatgtgcaggaacgaacgattttttttaaggacgacgggaattacaaaacccgcgccgaggttaagttcgagggcgataccctggttaatcggatagagctcaaaggaatagattttaaagaggacggtaacattctgggccacaagctcgaatataactataattcacacaacgtatacatcatggctgacaagcagaaaaacggcatcaaagttaactttaagatacgacacaacattgaagacgggtctgtgcagttggctgaccactaccagcagaacacgccgatcggggatggaccggtgctgctgcctgacaaccactacctctctacccagtctgccctgtctaaggaccctaacgaaaagcgggatcacatggtgcttcttgagttcgtcactgccgcaggaattacacacggtatggacgaactctataagtaataacgcgcgcgactgaatgcatgacgcgtaaaaaaaaaaatactagagtagcataaccccttggggcctctaaacgggtcttgaggggtttttt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z