##gff-version 3
##sequence-region BBa_K1442110 1 1498
BBa_K1442110	.	engineered_region	711	1444	.	+	0	ID=BBa_K1442106;Name=BBa_K1442106
BBa_K1442110	.	terminator	1537	1582	.	+	0	ID=BBa_K1442301;Name=BBa_K1442301;Parent=BBa_K1442106
BBa_K1442110	.	promoter	711	737	.	+	0	ID=BBa_K1442029;Name=BBa_K1442029;Parent=BBa_K1442106
BBa_K1442110	.	CDS	746	1528	.	+	0	ID=BBa_K1442308;Name=BBa_K1442308;Parent=BBa_K1442106
BBa_K1442110	.	polyA_site	1518	1528	.	+	0	ID=annotation2420100;Name=poly A tail ;Parent=BBa_K1442308
BBa_K1442110	.	stop_codon	1486	1488	.	+	0	ID=annotation2420095;Name=Stop codon;Parent=BBa_K1442308
BBa_K1442110	.	sequence_feature	766	771	.	+	0	ID=annotation2420093;Name=BsrGI restriction site ;Parent=BBa_K1442308
BBa_K1442110	.	sequence_feature	1512	1517	.	+	0	ID=annotation2420098;Name=MluI;Parent=BBa_K1442308
BBa_K1442110	.	CDS	772	1491	.	+	0	ID=annotation2420094;Name=GFP codon optimised for humans;Parent=BBa_K1442308
BBa_K1442110	.	sequence_feature	746	753	.	+	0	ID=annotation2420092;Name=AscI resctriction site;Parent=BBa_K1442308
BBa_K1442110	.	stop_codon	1489	1491	.	+	0	ID=annotation2420096;Name=Stop codon;Parent=BBa_K1442308
BBa_K1442110	.	sequence_feature	1492	1499	.	+	0	ID=annotation2420097;Name=MauBI restriciton site ;Parent=BBa_K1442308
BBa_K1442110	.	promoter	1	27	.	+	0	ID=BBa_K1442029;Name=BBa_K1442029
BBa_K1442110	.	terminator	1453	1498	.	+	0	ID=BBa_K1442301;Name=BBa_K1442301
BBa_K1442110	.	ribosome_entry_site	36	702	.	+	0	ID=BBa_K1442024;Name=BBa_K1442024
BBa_K1442110	.	sequence_feature	697	702	.	+	0	ID=annotation2399344;Name=BsrGI restriction site ;Parent=BBa_K1442024
BBa_K1442110	.	sequence_feature	36	43	.	+	0	ID=annotation2399332;Name=AscI restriction site ;Parent=BBa_K1442024
BBa_K1442110	.	ribosome_entry_site	44	696	.	+	0	ID=annotation2399343;Name=NKRF IRES;Parent=BBa_K1442024
>BBa_K1442110
gcgaaattaatacgactcactatagggtactagagggcgcgcccagagtaatgacatggttccttccatcctccaaagg
tgaccaataatagtttgtaagtatcattatgaactaatgaattttcaacatatttgatatatttcaatccattgccatc
attgttcttatcgatatttgagttggctcactttgccagtaagagtctattcaaattggcttctgagtccatttgacac
aacacctttgatctttgacagtttccttggttttaggtgctagatgatttctcaggctcaccttagacatttcctgcca
cagacttagaatcagccatttctctaaggaccctgattccatttcatgagaaatgatagagaccacaatcaaaacaagt
catgaatttatactgatattttcaattcaaattaaagatgaggtttttgctaaatttttttgagtttatatttgtatgt
cttatgctgaaaaatcttgtttcctaattagtaacataattattcatttgatgggtaaatattttagggccgattcttt
ggttttatagccaagataccctgttgataaagtcttgtgggagcaattataagactggcttattttgaagctttttaaa
aaagacatccttacctgttttaactgtagattatattaacttaaataggtacagcccacgcttgtgtacatactagagt
gtacaatgcggaaaggggaggagctcttcacaggcgtggtgcccatactggtggagttggacggcgacgtgaacggtca
caagttctcagtctctggggagggagagggcgatgccacctatggcaagcttacactgaagttcatttgtacgaccggc
aagctccctgtgccatggcccaccctggtcacaaccttcggctacggggtgcagtgctttgccagatatcccgaccaca
tgaagcagcatgacttctttaagagtgcaatgcccgaagggtatgtgcaggaacgaacgattttttttaaggacgacgg
gaattacaaaacccgcgccgaggttaagttcgagggcgataccctggttaatcggatagagctcaaaggaatagatttt
aaagaggacggtaacattctgggccacaagctcgaatataactataattcacacaacgtatacatcatggctgacaagc
agaaaaacggcatcaaagttaactttaagatacgacacaacattgaagacgggtctgtgcagttggctgaccactacca
gcagaacacgccgatcggggatggaccggtgctgctgcctgacaaccactacctctctacccagtctgccctgtctaag
gaccctaacgaaaagcgggatcacatggtgcttcttgagttcgtcactgccgcaggaattacacacggtatggacgaac
tctataagtaataacgcgcgcgtactagagtagcataaccccttggggcctctaaacgggtcttgaggggtttttt